Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thyA and Shew_1042 are separated by 306 nucleotides Shew_1042 and Shew_1043 overlap by 4 nucleotides Shew_1043 and nhaR are separated by 20 nucleotides
Shew_1041: thyA - thymidylate synthase (RefSeq), at 1,205,044 to 1,205,895
thyA
Shew_1042: Shew_1042 - Na+/H+ antiporter NhaA (RefSeq), at 1,206,202 to 1,207,377
_1042
Shew_1043: Shew_1043 - hypothetical protein (RefSeq), at 1,207,374 to 1,207,757
_1043
Shew_1044: nhaR - transcriptional activator NhaR (RefSeq), at 1,207,778 to 1,208,686
nhaR
Position (kb)
1206
1207
1208 Strain fitness (log2 ratio)
-1
0
1
2 at 1206.698 kb on + strand, within Shew_1042 at 1206.861 kb on - strand, within Shew_1042 at 1206.871 kb on - strand, within Shew_1042 at 1206.880 kb on + strand, within Shew_1042 at 1206.880 kb on + strand, within Shew_1042 at 1207.109 kb on + strand, within Shew_1042 at 1207.147 kb on - strand, within Shew_1042 at 1207.147 kb on - strand, within Shew_1042 at 1207.173 kb on - strand, within Shew_1042 at 1207.261 kb on + strand at 1207.747 kb on + strand at 1207.760 kb on + strand at 1207.865 kb on - strand at 1207.930 kb on - strand, within nhaR at 1208.162 kb on - strand, within nhaR at 1208.331 kb on + strand, within nhaR
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,206,698 + Shew_1042 0.42 +0.4 1,206,861 - Shew_1042 0.56 +0.7 1,206,871 - Shew_1042 0.57 +1.0 1,206,880 + Shew_1042 0.58 +0.1 1,206,880 + Shew_1042 0.58 -0.1 1,207,109 + Shew_1042 0.77 +0.1 1,207,147 - Shew_1042 0.80 +1.0 1,207,147 - Shew_1042 0.80 +0.4 1,207,173 - Shew_1042 0.83 +1.8 1,207,261 + +0.7 1,207,747 + -1.3 1,207,760 + +0.8 1,207,865 - +1.0 1,207,930 - nhaR Shew_1044 0.17 +0.0 1,208,162 - nhaR Shew_1044 0.42 +0.6 1,208,331 + nhaR Shew_1044 0.61 +0.4
Or see this region's nucleotide sequence