Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_637 and Pf1N1B4_638 are separated by 0 nucleotides Pf1N1B4_638 and Pf1N1B4_639 are separated by 115 nucleotides Pf1N1B4_639 and Pf1N1B4_640 are separated by 158 nucleotides
Pf1N1B4_637 - Holliday junction DNA helicase RuvB, at 732,917 to 733,978
_637
Pf1N1B4_638 - Holliday junction DNA helicase RuvA, at 733,979 to 734,596
_638
Pf1N1B4_639 - Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4), at 734,712 to 735,236
_639
Pf1N1B4_640 - FIG000859: hypothetical protein YebC, at 735,395 to 736,141
_640
Position (kb) 733
734
735 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 733.044 kb on + strand, within Pf1N1B4_637 at 733.062 kb on + strand, within Pf1N1B4_637 at 733.881 kb on + strand at 733.882 kb on - strand at 733.980 kb on + strand at 733.981 kb on - strand at 733.981 kb on - strand at 734.534 kb on + strand, within Pf1N1B4_638 at 734.711 kb on - strand at 735.081 kb on + strand, within Pf1N1B4_639 at 735.155 kb on - strand, within Pf1N1B4_639 at 735.201 kb on - strand at 735.228 kb on + strand at 735.228 kb on + strand at 735.254 kb on + strand at 735.254 kb on + strand at 735.254 kb on + strand at 735.254 kb on + strand at 735.254 kb on + strand at 735.254 kb on + strand at 735.254 kb on + strand at 735.255 kb on - strand at 735.273 kb on - strand at 735.386 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 733,044 + Pf1N1B4_637 0.12 -1.0 733,062 + Pf1N1B4_637 0.14 -1.6 733,881 + -2.2 733,882 - -1.0 733,980 + -3.6 733,981 - -1.0 733,981 - -1.0 734,534 + Pf1N1B4_638 0.90 -0.0 734,711 - -1.6 735,081 + Pf1N1B4_639 0.70 -0.0 735,155 - Pf1N1B4_639 0.84 -2.8 735,201 - -1.6 735,228 + -1.0 735,228 + -1.6 735,254 + -0.0 735,254 + +0.8 735,254 + -2.0 735,254 + -1.0 735,254 + -2.0 735,254 + -2.0 735,254 + -5.0 735,255 - -0.5 735,273 - -0.7 735,386 + -1.6
Or see this region's nucleotide sequence