Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5845 and Pf1N1B4_5846 are separated by 117 nucleotides Pf1N1B4_5846 and Pf1N1B4_5847 are separated by 101 nucleotides Pf1N1B4_5847 and Pf1N1B4_5848 are separated by 147 nucleotides Pf1N1B4_5848 and Pf1N1B4_5849 overlap by 1 nucleotides
Pf1N1B4_5845 - GMP synthase - Glutamine amidotransferase domain, at 279,680 to 280,321
_5845
Pf1N1B4_5846 - Rhodanese-related sulfurtransferase, at 280,439 to 280,873
_5846
Pf1N1B4_5847 - transcriptional activator FtrA, at 280,975 to 281,949
_5847
Pf1N1B4_5848 - Precorrin-6A synthase (EC 2.1.1.152), at 282,097 to 282,852
_5848
Pf1N1B4_5849 - Probable phosphoglycerate mutase, at 282,852 to 283,337
_5849
Position (kb) 280
281
282 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 280.369 kb on - strand at 280.369 kb on - strand at 280.369 kb on - strand at 280.403 kb on + strand at 280.404 kb on - strand at 280.612 kb on - strand, within Pf1N1B4_5846 at 280.774 kb on - strand, within Pf1N1B4_5846 at 280.880 kb on + strand at 280.880 kb on + strand at 280.881 kb on - strand at 280.914 kb on - strand at 280.914 kb on - strand at 281.011 kb on + strand at 281.012 kb on - strand at 281.197 kb on - strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.403 kb on - strand, within Pf1N1B4_5847 at 281.403 kb on - strand, within Pf1N1B4_5847 at 281.403 kb on - strand, within Pf1N1B4_5847 at 281.403 kb on - strand at 281.425 kb on + strand, within Pf1N1B4_5847 at 281.425 kb on + strand at 281.425 kb on + strand, within Pf1N1B4_5847 at 281.933 kb on + strand at 281.934 kb on - strand at 281.934 kb on - strand at 282.116 kb on + strand at 282.116 kb on + strand at 282.116 kb on + strand at 282.117 kb on - strand at 282.117 kb on - strand at 282.117 kb on - strand at 282.117 kb on - strand at 282.117 kb on - strand at 282.117 kb on - strand at 282.120 kb on + strand at 282.121 kb on - strand at 282.121 kb on - strand at 282.242 kb on + strand, within Pf1N1B4_5848 at 282.243 kb on - strand, within Pf1N1B4_5848 at 282.393 kb on + strand, within Pf1N1B4_5848 at 282.393 kb on + strand, within Pf1N1B4_5848 at 282.393 kb on + strand, within Pf1N1B4_5848 at 282.393 kb on + strand at 282.393 kb on + strand at 282.393 kb on + strand, within Pf1N1B4_5848 at 282.393 kb on + strand, within Pf1N1B4_5848 at 282.394 kb on - strand, within Pf1N1B4_5848 at 282.394 kb on - strand, within Pf1N1B4_5848 at 282.394 kb on - strand at 282.394 kb on - strand, within Pf1N1B4_5848 at 282.394 kb on - strand, within Pf1N1B4_5848 at 282.394 kb on - strand at 282.394 kb on - strand, within Pf1N1B4_5848 at 282.495 kb on + strand, within Pf1N1B4_5848 at 282.496 kb on - strand at 282.503 kb on + strand, within Pf1N1B4_5848 at 282.503 kb on + strand, within Pf1N1B4_5848 at 282.503 kb on + strand, within Pf1N1B4_5848 at 282.503 kb on + strand, within Pf1N1B4_5848 at 282.503 kb on + strand, within Pf1N1B4_5848 at 282.504 kb on - strand, within Pf1N1B4_5848 at 282.504 kb on - strand, within Pf1N1B4_5848 at 282.623 kb on + strand at 282.692 kb on + strand, within Pf1N1B4_5848 at 282.692 kb on + strand, within Pf1N1B4_5848 at 282.693 kb on - strand, within Pf1N1B4_5848 at 282.888 kb on + strand at 282.889 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 280,369 - +1.6 280,369 - -0.8 280,369 - +1.2 280,403 + -0.4 280,404 - +0.6 280,612 - Pf1N1B4_5846 0.40 -0.0 280,774 - Pf1N1B4_5846 0.77 -1.2 280,880 + -0.8 280,880 + -1.0 280,881 - -1.4 280,914 - -0.4 280,914 - -2.8 281,011 + +0.3 281,012 - -0.3 281,197 - Pf1N1B4_5847 0.23 -0.2 281,402 + Pf1N1B4_5847 0.44 -2.0 281,402 + Pf1N1B4_5847 0.44 -2.0 281,402 + Pf1N1B4_5847 0.44 -1.2 281,402 + Pf1N1B4_5847 0.44 +1.9 281,402 + Pf1N1B4_5847 0.44 -0.4 281,403 - Pf1N1B4_5847 0.44 -0.1 281,403 - Pf1N1B4_5847 0.44 -3.2 281,403 - Pf1N1B4_5847 0.44 -0.8 281,403 - +0.6 281,425 + Pf1N1B4_5847 0.46 -1.9 281,425 + -1.4 281,425 + Pf1N1B4_5847 0.46 -0.1 281,933 + -0.4 281,934 - +0.6 281,934 - -0.1 282,116 + -0.4 282,116 + -0.0 282,116 + -0.0 282,117 - -0.2 282,117 - -1.4 282,117 - +1.3 282,117 - -0.3 282,117 - +1.2 282,117 - -0.1 282,120 + -0.8 282,121 - -0.4 282,121 - -0.2 282,242 + Pf1N1B4_5848 0.19 -1.0 282,243 - Pf1N1B4_5848 0.19 -0.3 282,393 + Pf1N1B4_5848 0.39 +0.0 282,393 + Pf1N1B4_5848 0.39 +0.5 282,393 + Pf1N1B4_5848 0.39 -1.4 282,393 + -0.4 282,393 + -0.4 282,393 + Pf1N1B4_5848 0.39 +0.3 282,393 + Pf1N1B4_5848 0.39 +1.2 282,394 - Pf1N1B4_5848 0.39 +0.2 282,394 - Pf1N1B4_5848 0.39 +0.6 282,394 - +1.6 282,394 - Pf1N1B4_5848 0.39 +0.0 282,394 - Pf1N1B4_5848 0.39 -1.1 282,394 - -1.4 282,394 - Pf1N1B4_5848 0.39 -0.4 282,495 + Pf1N1B4_5848 0.53 -0.4 282,496 - -1.4 282,503 + Pf1N1B4_5848 0.54 +0.4 282,503 + Pf1N1B4_5848 0.54 +0.6 282,503 + Pf1N1B4_5848 0.54 +0.3 282,503 + Pf1N1B4_5848 0.54 -0.7 282,503 + Pf1N1B4_5848 0.54 -0.9 282,504 - Pf1N1B4_5848 0.54 +0.2 282,504 - Pf1N1B4_5848 0.54 -0.0 282,623 + +0.6 282,692 + Pf1N1B4_5848 0.79 -1.1 282,692 + Pf1N1B4_5848 0.79 +0.3 282,693 - Pf1N1B4_5848 0.79 +0.6 282,888 + +0.6 282,889 - +0.6
Or see this region's nucleotide sequence