Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5709 and Pf1N1B4_5710 are separated by 22 nucleotides Pf1N1B4_5710 and Pf1N1B4_5711 are separated by 92 nucleotides Pf1N1B4_5711 and Pf1N1B4_5712 are separated by 336 nucleotides
Pf1N1B4_5709 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 147,817 to 149,052
_5709
Pf1N1B4_5710 - Transcriptional regulator, TetR family, at 149,075 to 149,629
_5710
Pf1N1B4_5711 - Salicylate hydroxylase (EC 1.14.13.1), at 149,722 to 151,380
_5711
Pf1N1B4_5712 - Adenylate cyclase (EC 4.6.1.1) / Guanylate cyclase (EC 4.6.1.2), at 151,717 to 154,614
_5712
Position (kb) 149
150
151
152 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 149.026 kb on + strand at 149.121 kb on - strand at 149.175 kb on - strand, within Pf1N1B4_5710 at 149.219 kb on + strand, within Pf1N1B4_5710 at 149.219 kb on + strand, within Pf1N1B4_5710 at 149.526 kb on + strand, within Pf1N1B4_5710 at 149.527 kb on - strand, within Pf1N1B4_5710 at 149.732 kb on - strand at 149.856 kb on + strand at 150.374 kb on + strand, within Pf1N1B4_5711 at 150.374 kb on + strand, within Pf1N1B4_5711 at 150.374 kb on + strand, within Pf1N1B4_5711 at 150.374 kb on + strand, within Pf1N1B4_5711 at 150.385 kb on + strand, within Pf1N1B4_5711 at 150.489 kb on + strand at 150.809 kb on + strand, within Pf1N1B4_5711 at 150.809 kb on + strand, within Pf1N1B4_5711 at 150.810 kb on - strand at 150.810 kb on - strand, within Pf1N1B4_5711 at 151.014 kb on + strand, within Pf1N1B4_5711 at 151.014 kb on + strand, within Pf1N1B4_5711 at 151.256 kb on - strand at 151.506 kb on - strand at 151.526 kb on + strand at 151.526 kb on + strand at 151.527 kb on - strand at 151.685 kb on - strand at 151.799 kb on + strand at 151.799 kb on + strand at 151.853 kb on + strand at 152.171 kb on - strand, within Pf1N1B4_5712
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 149,026 + -1.0 149,121 - -1.5 149,175 - Pf1N1B4_5710 0.18 +0.4 149,219 + Pf1N1B4_5710 0.26 -2.8 149,219 + Pf1N1B4_5710 0.26 -1.0 149,526 + Pf1N1B4_5710 0.81 +0.1 149,527 - Pf1N1B4_5710 0.81 -0.3 149,732 - -0.8 149,856 + +0.1 150,374 + Pf1N1B4_5711 0.39 -0.5 150,374 + Pf1N1B4_5711 0.39 -0.5 150,374 + Pf1N1B4_5711 0.39 +2.0 150,374 + Pf1N1B4_5711 0.39 +1.9 150,385 + Pf1N1B4_5711 0.40 +0.5 150,489 + -0.5 150,809 + Pf1N1B4_5711 0.66 +1.0 150,809 + Pf1N1B4_5711 0.66 +0.8 150,810 - -2.0 150,810 - Pf1N1B4_5711 0.66 +0.5 151,014 + Pf1N1B4_5711 0.78 +0.3 151,014 + Pf1N1B4_5711 0.78 -1.5 151,256 - +1.5 151,506 - +0.1 151,526 + +0.5 151,526 + +1.1 151,527 - -1.0 151,685 - -0.5 151,799 + -0.0 151,799 + -0.7 151,853 + +1.1 152,171 - Pf1N1B4_5712 0.16 -0.9
Or see this region's nucleotide sequence