Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5680 and Pf1N1B4_5681 are separated by 7 nucleotides Pf1N1B4_5681 and Pf1N1B4_5682 overlap by 1 nucleotides Pf1N1B4_5682 and Pf1N1B4_5683 are separated by 87 nucleotides
Pf1N1B4_5680 - DNA polymerase-like protein PA0670, at 119,869 to 121,284
_5680
Pf1N1B4_5681 - RecA/RadA recombinase, at 121,292 to 121,909
_5681
Pf1N1B4_5682 - SOS-response repressor and protease LexA (EC 3.4.21.88), at 121,909 to 122,526
_5682
Pf1N1B4_5683 - Predicted dye-decolorizing peroxidase (DyP), YfeX-like subgroup, at 122,614 to 123,501
_5683
Position (kb)
121
122
123 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 121.071 kb on - strand, within Pf1N1B4_5680 at 121.986 kb on - strand, within Pf1N1B4_5682 at 122.002 kb on + strand at 122.134 kb on - strand, within Pf1N1B4_5682 at 122.174 kb on + strand, within Pf1N1B4_5682 at 122.175 kb on - strand, within Pf1N1B4_5682 at 122.175 kb on - strand, within Pf1N1B4_5682 at 122.507 kb on - strand at 122.507 kb on - strand at 122.507 kb on - strand at 122.673 kb on - strand at 123.362 kb on - strand, within Pf1N1B4_5683 at 123.444 kb on + strand at 123.444 kb on + strand at 123.445 kb on - strand at 123.445 kb on - strand at 123.445 kb on - strand at 123.445 kb on - strand at 123.494 kb on + strand at 123.495 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 121,071 - Pf1N1B4_5680 0.85 +0.1 121,986 - Pf1N1B4_5682 0.12 +1.1 122,002 + -1.0 122,134 - Pf1N1B4_5682 0.36 -0.5 122,174 + Pf1N1B4_5682 0.43 -2.2 122,175 - Pf1N1B4_5682 0.43 -2.3 122,175 - Pf1N1B4_5682 0.43 -0.9 122,507 - +0.5 122,507 - +0.5 122,507 - +0.2 122,673 - -0.0 123,362 - Pf1N1B4_5683 0.84 -1.5 123,444 + -1.9 123,444 + -1.0 123,445 - -3.0 123,445 - -1.5 123,445 - -2.0 123,445 - -1.5 123,494 + -0.5 123,495 - -1.5
Or see this region's nucleotide sequence