Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5678 and Pf1N1B4_5679 are separated by 179 nucleotides Pf1N1B4_5679 and Pf1N1B4_5680 overlap by 4 nucleotides
Pf1N1B4_5678 - sensory box histidine kinase/response regulator, at 114,552 to 116,615
_5678
Pf1N1B4_5679 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 116,795 to 119,872
_5679
Pf1N1B4_5680 - DNA polymerase-like protein PA0670, at 119,869 to 121,284
_5680
Position (kb)
116
117
118
119
120 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 115.867 kb on + strand at 115.906 kb on + strand, within Pf1N1B4_5678 at 115.906 kb on + strand at 116.260 kb on + strand, within Pf1N1B4_5678 at 116.260 kb on + strand at 116.261 kb on - strand, within Pf1N1B4_5678 at 116.422 kb on + strand at 116.453 kb on - strand at 116.463 kb on - strand at 116.464 kb on + strand at 116.464 kb on + strand at 116.464 kb on + strand at 116.473 kb on + strand at 116.474 kb on - strand at 116.523 kb on + strand at 116.633 kb on + strand at 116.633 kb on + strand at 116.775 kb on + strand at 116.775 kb on + strand at 116.778 kb on - strand at 116.778 kb on - strand at 116.933 kb on + strand at 117.094 kb on + strand at 117.214 kb on + strand, within Pf1N1B4_5679 at 117.615 kb on + strand at 117.615 kb on + strand, within Pf1N1B4_5679 at 117.615 kb on + strand, within Pf1N1B4_5679 at 117.693 kb on + strand, within Pf1N1B4_5679 at 117.694 kb on - strand, within Pf1N1B4_5679 at 117.738 kb on + strand at 117.739 kb on - strand, within Pf1N1B4_5679 at 117.965 kb on - strand, within Pf1N1B4_5679 at 117.965 kb on - strand at 117.965 kb on - strand, within Pf1N1B4_5679 at 118.000 kb on - strand, within Pf1N1B4_5679 at 118.021 kb on + strand, within Pf1N1B4_5679 at 118.288 kb on + strand, within Pf1N1B4_5679 at 118.660 kb on - strand, within Pf1N1B4_5679 at 118.660 kb on - strand, within Pf1N1B4_5679 at 118.660 kb on - strand, within Pf1N1B4_5679 at 118.660 kb on - strand, within Pf1N1B4_5679 at 118.660 kb on - strand, within Pf1N1B4_5679 at 118.713 kb on - strand, within Pf1N1B4_5679 at 118.713 kb on - strand, within Pf1N1B4_5679 at 118.716 kb on + strand, within Pf1N1B4_5679 at 118.716 kb on + strand, within Pf1N1B4_5679 at 118.717 kb on - strand, within Pf1N1B4_5679 at 118.851 kb on + strand, within Pf1N1B4_5679 at 118.852 kb on - strand, within Pf1N1B4_5679 at 119.418 kb on - strand, within Pf1N1B4_5679 at 119.418 kb on - strand, within Pf1N1B4_5679 at 119.418 kb on - strand, within Pf1N1B4_5679 at 119.557 kb on - strand, within Pf1N1B4_5679 at 119.667 kb on - strand at 119.960 kb on + strand at 119.961 kb on - strand at 119.964 kb on - strand at 120.098 kb on - strand at 120.098 kb on - strand, within Pf1N1B4_5680 at 120.098 kb on - strand, within Pf1N1B4_5680 at 120.256 kb on + strand, within Pf1N1B4_5680 at 120.594 kb on - strand, within Pf1N1B4_5680 at 120.594 kb on - strand, within Pf1N1B4_5680 at 120.616 kb on + strand, within Pf1N1B4_5680 at 120.675 kb on + strand, within Pf1N1B4_5680 at 120.675 kb on + strand, within Pf1N1B4_5680 at 120.676 kb on - strand, within Pf1N1B4_5680 at 120.818 kb on - strand at 120.818 kb on - strand, within Pf1N1B4_5680
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 115,867 + +0.5 115,906 + Pf1N1B4_5678 0.66 +0.7 115,906 + -1.5 116,260 + Pf1N1B4_5678 0.83 +0.3 116,260 + -0.5 116,261 - Pf1N1B4_5678 0.83 +0.2 116,422 + +2.4 116,453 - +0.5 116,463 - -1.6 116,464 + -1.5 116,464 + -0.4 116,464 + +0.7 116,473 + -0.3 116,474 - -1.8 116,523 + +2.2 116,633 + -1.8 116,633 + -1.5 116,775 + +0.1 116,775 + +0.2 116,778 - +0.4 116,778 - -3.0 116,933 + +0.1 117,094 + +1.0 117,214 + Pf1N1B4_5679 0.14 -0.9 117,615 + +2.2 117,615 + Pf1N1B4_5679 0.27 -0.2 117,615 + Pf1N1B4_5679 0.27 -0.3 117,693 + Pf1N1B4_5679 0.29 +0.4 117,694 - Pf1N1B4_5679 0.29 -2.5 117,738 + -1.5 117,739 - Pf1N1B4_5679 0.31 -0.9 117,965 - Pf1N1B4_5679 0.38 -2.0 117,965 - -1.5 117,965 - Pf1N1B4_5679 0.38 +0.3 118,000 - Pf1N1B4_5679 0.39 -3.0 118,021 + Pf1N1B4_5679 0.40 +0.1 118,288 + Pf1N1B4_5679 0.49 +0.4 118,660 - Pf1N1B4_5679 0.61 -0.5 118,660 - Pf1N1B4_5679 0.61 -1.5 118,660 - Pf1N1B4_5679 0.61 -0.5 118,660 - Pf1N1B4_5679 0.61 +0.3 118,660 - Pf1N1B4_5679 0.61 +0.8 118,713 - Pf1N1B4_5679 0.62 -0.5 118,713 - Pf1N1B4_5679 0.62 +0.5 118,716 + Pf1N1B4_5679 0.62 +0.5 118,716 + Pf1N1B4_5679 0.62 +1.5 118,717 - Pf1N1B4_5679 0.62 +0.1 118,851 + Pf1N1B4_5679 0.67 +0.4 118,852 - Pf1N1B4_5679 0.67 -0.7 119,418 - Pf1N1B4_5679 0.85 +1.1 119,418 - Pf1N1B4_5679 0.85 -1.5 119,418 - Pf1N1B4_5679 0.85 +0.1 119,557 - Pf1N1B4_5679 0.90 +2.0 119,667 - -0.0 119,960 + +0.9 119,961 - +0.1 119,964 - -2.0 120,098 - +0.5 120,098 - Pf1N1B4_5680 0.16 +0.8 120,098 - Pf1N1B4_5680 0.16 +0.5 120,256 + Pf1N1B4_5680 0.27 +0.1 120,594 - Pf1N1B4_5680 0.51 +1.9 120,594 - Pf1N1B4_5680 0.51 -0.5 120,616 + Pf1N1B4_5680 0.53 +1.5 120,675 + Pf1N1B4_5680 0.57 +0.1 120,675 + Pf1N1B4_5680 0.57 -2.0 120,676 - Pf1N1B4_5680 0.57 -0.5 120,818 - -0.5 120,818 - Pf1N1B4_5680 0.67 -0.6
Or see this region's nucleotide sequence