Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5661 and Pf1N1B4_5662 are separated by 156 nucleotides Pf1N1B4_5662 and Pf1N1B4_5663 are separated by 6 nucleotides Pf1N1B4_5663 and Pf1N1B4_5664 are separated by 191 nucleotides Pf1N1B4_5664 and Pf1N1B4_5665 are separated by 291 nucleotides
Pf1N1B4_5661 - 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13), at 93,044 to 96,754
_5661
Pf1N1B4_5662 - FIG00953703: hypothetical protein, at 96,911 to 97,132
_5662
Pf1N1B4_5663 - Periplasmic sugar-binding domain protein, at 97,139 to 98,149
_5663
Pf1N1B4_5664 - hypothetical protein, at 98,341 to 98,454
_5664
Pf1N1B4_5665 - Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2), at 98,746 to 100,404
_5665
Position (kb)
97
98
99 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 96.224 kb on + strand, within Pf1N1B4_5661 at 96.302 kb on + strand, within Pf1N1B4_5661 at 96.302 kb on + strand, within Pf1N1B4_5661 at 96.302 kb on + strand, within Pf1N1B4_5661 at 96.379 kb on + strand, within Pf1N1B4_5661 at 96.386 kb on + strand at 96.386 kb on + strand at 96.386 kb on + strand at 96.443 kb on + strand at 96.443 kb on + strand at 96.443 kb on + strand at 96.443 kb on + strand at 96.443 kb on + strand at 96.443 kb on + strand at 96.443 kb on + strand at 96.444 kb on - strand at 96.444 kb on - strand at 96.444 kb on - strand at 96.444 kb on - strand at 96.578 kb on + strand at 96.614 kb on + strand at 96.615 kb on - strand at 96.615 kb on - strand at 96.639 kb on - strand at 96.680 kb on + strand at 96.680 kb on + strand at 96.680 kb on + strand at 96.680 kb on + strand at 96.680 kb on + strand at 96.680 kb on + strand at 96.680 kb on + strand at 96.753 kb on - strand at 96.868 kb on + strand at 96.869 kb on - strand at 96.869 kb on - strand at 97.075 kb on - strand, within Pf1N1B4_5662 at 97.444 kb on + strand, within Pf1N1B4_5663 at 97.498 kb on - strand, within Pf1N1B4_5663 at 97.789 kb on - strand, within Pf1N1B4_5663 at 97.789 kb on - strand, within Pf1N1B4_5663 at 97.789 kb on - strand, within Pf1N1B4_5663 at 97.837 kb on - strand, within Pf1N1B4_5663 at 97.950 kb on + strand, within Pf1N1B4_5663 at 97.950 kb on + strand, within Pf1N1B4_5663 at 98.103 kb on + strand at 98.192 kb on + strand at 98.208 kb on + strand at 98.209 kb on - strand at 98.209 kb on - strand at 98.260 kb on - strand at 98.260 kb on - strand at 98.342 kb on + strand at 98.343 kb on - strand at 98.609 kb on - strand at 98.622 kb on + strand at 98.623 kb on - strand at 98.623 kb on - strand at 98.709 kb on + strand at 98.710 kb on - strand at 98.710 kb on - strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.743 kb on + strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.744 kb on - strand at 98.745 kb on + strand at 98.745 kb on + strand at 98.745 kb on + strand at 98.745 kb on + strand at 98.745 kb on + strand at 98.745 kb on + strand at 98.745 kb on + strand at 98.745 kb on + strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.746 kb on - strand at 98.749 kb on + strand at 98.749 kb on + strand at 98.749 kb on + strand at 98.750 kb on - strand at 98.750 kb on - strand at 98.750 kb on - strand at 98.750 kb on - strand at 98.750 kb on - strand at 98.750 kb on - strand at 98.750 kb on - strand at 98.750 kb on - strand at 98.753 kb on + strand at 98.753 kb on + strand at 98.753 kb on + strand at 98.754 kb on - strand at 98.754 kb on - strand at 98.754 kb on - strand at 98.754 kb on - strand at 98.754 kb on - strand at 98.755 kb on + strand at 98.756 kb on - strand at 98.756 kb on - strand at 98.764 kb on + strand at 98.881 kb on + strand at 98.881 kb on + strand at 98.882 kb on - strand at 98.882 kb on - strand at 98.882 kb on - strand at 98.882 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 96,224 + Pf1N1B4_5661 0.86 -0.5 96,302 + Pf1N1B4_5661 0.88 -0.5 96,302 + Pf1N1B4_5661 0.88 -2.0 96,302 + Pf1N1B4_5661 0.88 -1.2 96,379 + Pf1N1B4_5661 0.90 -1.5 96,386 + +0.4 96,386 + +0.2 96,386 + +0.5 96,443 + +3.0 96,443 + +0.9 96,443 + -0.1 96,443 + +0.9 96,443 + -0.5 96,443 + -1.0 96,443 + +1.1 96,444 - +1.1 96,444 - -1.2 96,444 - -1.0 96,444 - -0.0 96,578 + +0.8 96,614 + -1.5 96,615 - +0.4 96,615 - +0.2 96,639 - +0.5 96,680 + +1.1 96,680 + +1.9 96,680 + -2.0 96,680 + -1.5 96,680 + +0.4 96,680 + +1.7 96,680 + +0.3 96,753 - -0.7 96,868 + -1.8 96,869 - -2.5 96,869 - +2.1 97,075 - Pf1N1B4_5662 0.74 +1.5 97,444 + Pf1N1B4_5663 0.30 -0.0 97,498 - Pf1N1B4_5663 0.36 -1.5 97,789 - Pf1N1B4_5663 0.64 -2.0 97,789 - Pf1N1B4_5663 0.64 -2.3 97,789 - Pf1N1B4_5663 0.64 -0.2 97,837 - Pf1N1B4_5663 0.69 +0.9 97,950 + Pf1N1B4_5663 0.80 -1.5 97,950 + Pf1N1B4_5663 0.80 -0.1 98,103 + -0.8 98,192 + +2.1 98,208 + +0.7 98,209 - -0.5 98,209 - +0.5 98,260 - +1.1 98,260 - -0.5 98,342 + +0.8 98,343 - -0.8 98,609 - -0.9 98,622 + +0.3 98,623 - -3.0 98,623 - -0.5 98,709 + +1.9 98,710 - +0.1 98,710 - +2.1 98,743 + +0.1 98,743 + -0.2 98,743 + -2.5 98,743 + +2.7 98,743 + -2.5 98,743 + -0.1 98,743 + -0.5 98,743 + +0.2 98,743 + -1.5 98,744 - +0.4 98,744 - -1.0 98,744 - +0.3 98,744 - -1.8 98,744 - +1.4 98,744 - +1.1 98,744 - +1.0 98,744 - +1.5 98,744 - +0.1 98,745 + +1.4 98,745 + +1.5 98,745 + +0.0 98,745 + +0.3 98,745 + +1.9 98,745 + -1.5 98,745 + +1.1 98,745 + -2.5 98,746 - +0.9 98,746 - -0.9 98,746 - -0.5 98,746 - +0.4 98,746 - +0.6 98,746 - +1.9 98,746 - -0.0 98,746 - -0.7 98,746 - -0.7 98,746 - -0.5 98,746 - +0.5 98,746 - -0.3 98,746 - +1.2 98,746 - -0.1 98,746 - +0.7 98,749 + -0.5 98,749 + -0.5 98,749 + -2.0 98,750 - -0.0 98,750 - -2.8 98,750 - -1.5 98,750 - -3.3 98,750 - +2.0 98,750 - +0.1 98,750 - -1.5 98,750 - -0.5 98,753 + +1.0 98,753 + -1.5 98,753 + +1.5 98,754 - -0.0 98,754 - -0.9 98,754 - +1.9 98,754 - +1.1 98,754 - -2.0 98,755 + -0.3 98,756 - -0.5 98,756 - +0.8 98,764 + +0.3 98,881 + +0.4 98,881 + +0.5 98,882 - -0.6 98,882 - +0.9 98,882 - +0.2 98,882 - -0.5
Or see this region's nucleotide sequence