Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5549 and Pf1N1B4_5550 are separated by 322 nucleotides Pf1N1B4_5550 and Pf1N1B4_5551 are separated by 148 nucleotides
Pf1N1B4_5549 - hypothetical protein, at 320,933 to 321,061
_5549
Pf1N1B4_5550 - Group 2 RNA polymerase sigma factor @ Cyanobacteria-specific RpoD-like sigma factor, type-7, at 321,384 to 322,937
_5550
Pf1N1B4_5551 - hypothetical protein, at 323,086 to 324,072
_5551
Position (kb) 321
322
323 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 320.409 kb on - strand at 320.558 kb on + strand at 320.558 kb on + strand at 320.559 kb on - strand at 320.622 kb on + strand at 320.622 kb on + strand at 320.622 kb on + strand at 320.623 kb on - strand at 320.623 kb on - strand at 320.623 kb on - strand at 320.681 kb on + strand at 320.682 kb on - strand at 320.682 kb on - strand at 320.682 kb on - strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.735 kb on - strand at 320.787 kb on + strand at 320.788 kb on - strand at 320.788 kb on - strand at 320.848 kb on + strand at 320.849 kb on - strand at 320.935 kb on - strand at 320.995 kb on + strand, within Pf1N1B4_5549 at 320.995 kb on + strand, within Pf1N1B4_5549 at 320.995 kb on + strand, within Pf1N1B4_5549 at 320.996 kb on - strand, within Pf1N1B4_5549 at 320.996 kb on - strand, within Pf1N1B4_5549 at 320.996 kb on - strand, within Pf1N1B4_5549 at 320.996 kb on - strand, within Pf1N1B4_5549 at 321.053 kb on - strand at 321.053 kb on - strand at 321.208 kb on + strand at 321.223 kb on + strand at 321.427 kb on - strand at 321.427 kb on - strand at 321.435 kb on + strand at 321.595 kb on + strand, within Pf1N1B4_5550 at 321.595 kb on + strand, within Pf1N1B4_5550 at 321.596 kb on - strand, within Pf1N1B4_5550 at 321.596 kb on - strand, within Pf1N1B4_5550 at 321.596 kb on - strand, within Pf1N1B4_5550 at 321.596 kb on - strand, within Pf1N1B4_5550 at 321.596 kb on - strand, within Pf1N1B4_5550 at 321.628 kb on - strand, within Pf1N1B4_5550 at 321.628 kb on - strand, within Pf1N1B4_5550 at 321.724 kb on + strand, within Pf1N1B4_5550 at 321.724 kb on + strand at 321.734 kb on - strand, within Pf1N1B4_5550 at 321.926 kb on - strand, within Pf1N1B4_5550 at 321.926 kb on - strand, within Pf1N1B4_5550 at 321.926 kb on - strand, within Pf1N1B4_5550 at 321.958 kb on + strand, within Pf1N1B4_5550 at 321.959 kb on - strand, within Pf1N1B4_5550 at 321.959 kb on - strand, within Pf1N1B4_5550 at 321.959 kb on - strand, within Pf1N1B4_5550 at 322.013 kb on + strand, within Pf1N1B4_5550 at 322.014 kb on - strand, within Pf1N1B4_5550 at 322.014 kb on - strand, within Pf1N1B4_5550 at 322.014 kb on - strand, within Pf1N1B4_5550 at 322.031 kb on - strand, within Pf1N1B4_5550 at 322.201 kb on - strand, within Pf1N1B4_5550 at 322.201 kb on - strand, within Pf1N1B4_5550 at 322.230 kb on - strand, within Pf1N1B4_5550 at 322.230 kb on - strand, within Pf1N1B4_5550 at 322.280 kb on - strand, within Pf1N1B4_5550 at 322.280 kb on - strand, within Pf1N1B4_5550 at 322.320 kb on + strand, within Pf1N1B4_5550 at 322.320 kb on + strand, within Pf1N1B4_5550 at 322.321 kb on - strand, within Pf1N1B4_5550 at 322.437 kb on - strand, within Pf1N1B4_5550 at 322.490 kb on + strand, within Pf1N1B4_5550 at 322.636 kb on + strand, within Pf1N1B4_5550 at 322.734 kb on - strand, within Pf1N1B4_5550 at 322.801 kb on - strand at 322.839 kb on + strand at 322.839 kb on + strand at 322.878 kb on + strand at 322.878 kb on + strand at 322.879 kb on - strand at 322.879 kb on - strand at 322.903 kb on - strand at 322.937 kb on + strand at 322.937 kb on + strand at 322.937 kb on + strand at 322.937 kb on + strand at 322.937 kb on + strand at 322.937 kb on + strand at 322.937 kb on + strand at 322.938 kb on - strand at 322.938 kb on - strand at 322.938 kb on - strand at 322.938 kb on - strand at 322.938 kb on - strand at 323.049 kb on + strand at 323.049 kb on + strand at 323.093 kb on - strand at 323.093 kb on - strand at 323.181 kb on + strand at 323.208 kb on + strand, within Pf1N1B4_5551 at 323.224 kb on + strand, within Pf1N1B4_5551 at 323.224 kb on + strand, within Pf1N1B4_5551 at 323.224 kb on + strand, within Pf1N1B4_5551 at 323.224 kb on + strand, within Pf1N1B4_5551 at 323.225 kb on - strand, within Pf1N1B4_5551 at 323.225 kb on - strand, within Pf1N1B4_5551 at 323.243 kb on + strand, within Pf1N1B4_5551 at 323.243 kb on + strand, within Pf1N1B4_5551 at 323.464 kb on - strand, within Pf1N1B4_5551 at 323.464 kb on - strand, within Pf1N1B4_5551 at 323.498 kb on + strand, within Pf1N1B4_5551 at 323.499 kb on - strand, within Pf1N1B4_5551 at 323.564 kb on - strand, within Pf1N1B4_5551 at 323.576 kb on + strand, within Pf1N1B4_5551 at 323.576 kb on + strand, within Pf1N1B4_5551 at 323.576 kb on + strand at 323.577 kb on - strand, within Pf1N1B4_5551 at 323.577 kb on - strand, within Pf1N1B4_5551 at 323.577 kb on - strand, within Pf1N1B4_5551 at 323.791 kb on + strand, within Pf1N1B4_5551 at 323.791 kb on + strand, within Pf1N1B4_5551 at 323.791 kb on + strand, within Pf1N1B4_5551 at 323.792 kb on - strand, within Pf1N1B4_5551 at 323.935 kb on + strand, within Pf1N1B4_5551
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 320,409 - -0.4 320,558 + +0.8 320,558 + +0.7 320,559 - +1.0 320,622 + +1.2 320,622 + +1.4 320,622 + -0.0 320,623 - +2.5 320,623 - +0.9 320,623 - +1.8 320,681 + -2.1 320,682 - +0.9 320,682 - -2.1 320,682 - -0.2 320,685 + -0.6 320,685 + -1.2 320,685 + -0.1 320,685 + -0.4 320,685 + +0.7 320,685 + +1.0 320,686 - -0.4 320,686 - +1.1 320,686 - +1.2 320,686 - +1.3 320,686 - +0.9 320,686 - -0.1 320,735 - +0.2 320,787 + -1.8 320,788 - +1.5 320,788 - +2.9 320,848 + +1.5 320,849 - +1.3 320,935 - +1.5 320,995 + Pf1N1B4_5549 0.48 +0.2 320,995 + Pf1N1B4_5549 0.48 +3.0 320,995 + Pf1N1B4_5549 0.48 +0.9 320,996 - Pf1N1B4_5549 0.49 +1.4 320,996 - Pf1N1B4_5549 0.49 +0.5 320,996 - Pf1N1B4_5549 0.49 -0.3 320,996 - Pf1N1B4_5549 0.49 +1.2 321,053 - -0.1 321,053 - +1.4 321,208 + -0.8 321,223 + +0.8 321,427 - -1.4 321,427 - +1.0 321,435 + -0.2 321,595 + Pf1N1B4_5550 0.14 -0.2 321,595 + Pf1N1B4_5550 0.14 +0.2 321,596 - Pf1N1B4_5550 0.14 -1.8 321,596 - Pf1N1B4_5550 0.14 +0.2 321,596 - Pf1N1B4_5550 0.14 +0.5 321,596 - Pf1N1B4_5550 0.14 +1.9 321,596 - Pf1N1B4_5550 0.14 +0.2 321,628 - Pf1N1B4_5550 0.16 +0.5 321,628 - Pf1N1B4_5550 0.16 +0.9 321,724 + Pf1N1B4_5550 0.22 -2.4 321,724 + +0.8 321,734 - Pf1N1B4_5550 0.23 +0.7 321,926 - Pf1N1B4_5550 0.35 -1.4 321,926 - Pf1N1B4_5550 0.35 -0.1 321,926 - Pf1N1B4_5550 0.35 +1.4 321,958 + Pf1N1B4_5550 0.37 -0.8 321,959 - Pf1N1B4_5550 0.37 -2.6 321,959 - Pf1N1B4_5550 0.37 +0.7 321,959 - Pf1N1B4_5550 0.37 +0.2 322,013 + Pf1N1B4_5550 0.40 -0.2 322,014 - Pf1N1B4_5550 0.41 -2.1 322,014 - Pf1N1B4_5550 0.41 +1.4 322,014 - Pf1N1B4_5550 0.41 -0.8 322,031 - Pf1N1B4_5550 0.42 +0.2 322,201 - Pf1N1B4_5550 0.53 +3.3 322,201 - Pf1N1B4_5550 0.53 +0.8 322,230 - Pf1N1B4_5550 0.54 +0.6 322,230 - Pf1N1B4_5550 0.54 -0.5 322,280 - Pf1N1B4_5550 0.58 +0.7 322,280 - Pf1N1B4_5550 0.58 -1.6 322,320 + Pf1N1B4_5550 0.60 +0.0 322,320 + Pf1N1B4_5550 0.60 -0.6 322,321 - Pf1N1B4_5550 0.60 +0.8 322,437 - Pf1N1B4_5550 0.68 -0.2 322,490 + Pf1N1B4_5550 0.71 -1.4 322,636 + Pf1N1B4_5550 0.81 -0.1 322,734 - Pf1N1B4_5550 0.87 +0.4 322,801 - +0.7 322,839 + +0.2 322,839 + -0.9 322,878 + -1.8 322,878 + -0.1 322,879 - +0.7 322,879 - +2.2 322,903 - -1.8 322,937 + -0.8 322,937 + -2.4 322,937 + -0.7 322,937 + -0.8 322,937 + -0.2 322,937 + +0.8 322,937 + -0.6 322,938 - +0.5 322,938 - +2.5 322,938 - -2.3 322,938 - +0.9 322,938 - -0.5 323,049 + +0.2 323,049 + -0.3 323,093 - -0.0 323,093 - +1.5 323,181 + -0.3 323,208 + Pf1N1B4_5551 0.12 -0.6 323,224 + Pf1N1B4_5551 0.14 +0.2 323,224 + Pf1N1B4_5551 0.14 -0.8 323,224 + Pf1N1B4_5551 0.14 -0.2 323,224 + Pf1N1B4_5551 0.14 +1.1 323,225 - Pf1N1B4_5551 0.14 +2.2 323,225 - Pf1N1B4_5551 0.14 -0.1 323,243 + Pf1N1B4_5551 0.16 +0.5 323,243 + Pf1N1B4_5551 0.16 -0.2 323,464 - Pf1N1B4_5551 0.38 -0.8 323,464 - Pf1N1B4_5551 0.38 -1.4 323,498 + Pf1N1B4_5551 0.42 +0.5 323,499 - Pf1N1B4_5551 0.42 -1.0 323,564 - Pf1N1B4_5551 0.48 -0.8 323,576 + Pf1N1B4_5551 0.50 -0.8 323,576 + Pf1N1B4_5551 0.50 -0.8 323,576 + +0.2 323,577 - Pf1N1B4_5551 0.50 -0.8 323,577 - Pf1N1B4_5551 0.50 +0.5 323,577 - Pf1N1B4_5551 0.50 -0.4 323,791 + Pf1N1B4_5551 0.71 -0.6 323,791 + Pf1N1B4_5551 0.71 -1.8 323,791 + Pf1N1B4_5551 0.71 +0.7 323,792 - Pf1N1B4_5551 0.72 +0.6 323,935 + Pf1N1B4_5551 0.86 +0.4
Or see this region's nucleotide sequence