Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_546 and Pf1N1B4_547 are separated by 229 nucleotides Pf1N1B4_547 and Pf1N1B4_548 are separated by 49 nucleotides Pf1N1B4_548 and Pf1N1B4_549 are separated by 14 nucleotides
Pf1N1B4_546 - FMN-dependent NADH-azoreductase, at 644,148 to 644,747
_546
Pf1N1B4_547 - 3-phosphoglycerate kinase, at 644,977 to 645,306
_547
Pf1N1B4_548 - Transcriptional regulator, LysR family, at 645,356 to 646,279
_548
Pf1N1B4_549 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 646,294 to 647,097
_549
Position (kb) 644
645
646 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 643.977 kb on - strand at 643.977 kb on - strand at 643.977 kb on - strand at 644.115 kb on - strand at 644.115 kb on - strand at 644.130 kb on + strand at 644.131 kb on - strand at 644.281 kb on + strand, within Pf1N1B4_546 at 644.281 kb on + strand, within Pf1N1B4_546 at 644.281 kb on + strand, within Pf1N1B4_546 at 644.281 kb on + strand, within Pf1N1B4_546 at 644.282 kb on - strand, within Pf1N1B4_546 at 644.282 kb on - strand, within Pf1N1B4_546 at 644.282 kb on - strand, within Pf1N1B4_546 at 644.282 kb on - strand, within Pf1N1B4_546 at 644.314 kb on + strand, within Pf1N1B4_546 at 644.315 kb on - strand, within Pf1N1B4_546 at 644.315 kb on - strand, within Pf1N1B4_546 at 644.341 kb on + strand, within Pf1N1B4_546 at 644.341 kb on + strand, within Pf1N1B4_546 at 644.342 kb on - strand, within Pf1N1B4_546 at 644.342 kb on - strand, within Pf1N1B4_546 at 644.342 kb on - strand, within Pf1N1B4_546 at 644.386 kb on + strand, within Pf1N1B4_546 at 644.386 kb on + strand, within Pf1N1B4_546 at 644.387 kb on - strand, within Pf1N1B4_546 at 644.387 kb on - strand, within Pf1N1B4_546 at 644.458 kb on + strand, within Pf1N1B4_546 at 644.459 kb on - strand, within Pf1N1B4_546 at 644.459 kb on - strand, within Pf1N1B4_546 at 644.476 kb on - strand, within Pf1N1B4_546 at 644.481 kb on + strand, within Pf1N1B4_546 at 644.481 kb on + strand, within Pf1N1B4_546 at 644.481 kb on + strand, within Pf1N1B4_546 at 644.482 kb on - strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.616 kb on - strand at 644.616 kb on - strand, within Pf1N1B4_546 at 644.616 kb on - strand, within Pf1N1B4_546 at 644.616 kb on - strand, within Pf1N1B4_546 at 644.616 kb on - strand, within Pf1N1B4_546 at 644.616 kb on - strand, within Pf1N1B4_546 at 644.754 kb on + strand at 644.754 kb on + strand at 644.754 kb on + strand at 644.754 kb on + strand at 644.754 kb on + strand at 644.754 kb on + strand at 644.754 kb on + strand at 644.754 kb on + strand at 644.754 kb on + strand at 644.755 kb on - strand at 644.755 kb on - strand at 644.776 kb on + strand at 644.776 kb on + strand at 644.776 kb on + strand at 644.776 kb on + strand at 644.776 kb on + strand at 644.776 kb on + strand at 644.777 kb on - strand at 644.777 kb on - strand at 644.777 kb on - strand at 644.828 kb on + strand at 644.972 kb on + strand at 644.972 kb on + strand at 644.972 kb on + strand at 644.972 kb on + strand at 644.972 kb on + strand at 644.972 kb on + strand at 644.972 kb on + strand at 644.973 kb on - strand at 644.973 kb on - strand at 645.003 kb on + strand at 645.004 kb on - strand at 645.140 kb on + strand, within Pf1N1B4_547 at 645.177 kb on + strand, within Pf1N1B4_547 at 645.177 kb on + strand at 645.177 kb on + strand, within Pf1N1B4_547 at 645.178 kb on - strand, within Pf1N1B4_547 at 645.178 kb on - strand, within Pf1N1B4_547 at 645.191 kb on + strand, within Pf1N1B4_547 at 645.192 kb on - strand, within Pf1N1B4_547 at 645.237 kb on - strand, within Pf1N1B4_547 at 645.291 kb on + strand at 645.291 kb on + strand at 645.292 kb on - strand at 645.292 kb on - strand at 645.292 kb on - strand at 645.292 kb on - strand at 645.337 kb on - strand at 645.337 kb on - strand at 645.337 kb on - strand at 645.397 kb on - strand at 645.397 kb on - strand at 645.397 kb on - strand at 645.397 kb on - strand at 645.470 kb on + strand, within Pf1N1B4_548 at 645.470 kb on + strand, within Pf1N1B4_548 at 645.471 kb on - strand at 645.471 kb on - strand, within Pf1N1B4_548 at 645.513 kb on + strand, within Pf1N1B4_548 at 646.122 kb on + strand, within Pf1N1B4_548 at 646.160 kb on + strand, within Pf1N1B4_548 at 646.160 kb on + strand, within Pf1N1B4_548 at 646.160 kb on + strand, within Pf1N1B4_548 at 646.161 kb on - strand, within Pf1N1B4_548 at 646.161 kb on - strand, within Pf1N1B4_548 at 646.161 kb on - strand, within Pf1N1B4_548 at 646.161 kb on - strand, within Pf1N1B4_548 at 646.161 kb on - strand, within Pf1N1B4_548 at 646.161 kb on - strand, within Pf1N1B4_548 at 646.161 kb on - strand, within Pf1N1B4_548 at 646.166 kb on + strand, within Pf1N1B4_548 at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.293 kb on + strand at 646.293 kb on + strand at 646.293 kb on + strand at 646.293 kb on + strand at 646.293 kb on + strand at 646.293 kb on + strand at 646.293 kb on + strand at 646.294 kb on - strand at 646.294 kb on - strand at 646.294 kb on - strand at 646.294 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 643,977 - -2.0 643,977 - +0.5 643,977 - -0.4 644,115 - +2.3 644,115 - -3.0 644,130 + -1.5 644,131 - -1.9 644,281 + Pf1N1B4_546 0.22 -0.6 644,281 + Pf1N1B4_546 0.22 -1.0 644,281 + Pf1N1B4_546 0.22 +0.4 644,281 + Pf1N1B4_546 0.22 -1.9 644,282 - Pf1N1B4_546 0.22 +0.8 644,282 - Pf1N1B4_546 0.22 -1.6 644,282 - Pf1N1B4_546 0.22 -1.8 644,282 - Pf1N1B4_546 0.22 +0.2 644,314 + Pf1N1B4_546 0.28 -1.6 644,315 - Pf1N1B4_546 0.28 -0.5 644,315 - Pf1N1B4_546 0.28 -0.5 644,341 + Pf1N1B4_546 0.32 -1.0 644,341 + Pf1N1B4_546 0.32 -2.0 644,342 - Pf1N1B4_546 0.32 +0.2 644,342 - Pf1N1B4_546 0.32 -1.0 644,342 - Pf1N1B4_546 0.32 -0.5 644,386 + Pf1N1B4_546 0.40 -1.4 644,386 + Pf1N1B4_546 0.40 -0.0 644,387 - Pf1N1B4_546 0.40 -0.8 644,387 - Pf1N1B4_546 0.40 +0.9 644,458 + Pf1N1B4_546 0.52 -1.8 644,459 - Pf1N1B4_546 0.52 -0.5 644,459 - Pf1N1B4_546 0.52 -1.0 644,476 - Pf1N1B4_546 0.55 -2.0 644,481 + Pf1N1B4_546 0.56 -1.0 644,481 + Pf1N1B4_546 0.56 +0.6 644,481 + Pf1N1B4_546 0.56 -0.4 644,482 - Pf1N1B4_546 0.56 +1.0 644,615 + Pf1N1B4_546 0.78 -2.0 644,615 + Pf1N1B4_546 0.78 -1.4 644,615 + Pf1N1B4_546 0.78 -1.3 644,615 + Pf1N1B4_546 0.78 +1.0 644,615 + Pf1N1B4_546 0.78 +0.4 644,615 + Pf1N1B4_546 0.78 -0.2 644,615 + Pf1N1B4_546 0.78 -0.5 644,615 + Pf1N1B4_546 0.78 -1.0 644,616 - -0.0 644,616 - Pf1N1B4_546 0.78 -1.0 644,616 - Pf1N1B4_546 0.78 +0.5 644,616 - Pf1N1B4_546 0.78 -1.0 644,616 - Pf1N1B4_546 0.78 -0.0 644,616 - Pf1N1B4_546 0.78 -0.7 644,754 + -1.0 644,754 + -0.3 644,754 + -1.7 644,754 + -1.6 644,754 + +0.4 644,754 + -1.4 644,754 + -0.0 644,754 + -0.8 644,754 + -1.5 644,755 - -1.0 644,755 - +0.8 644,776 + -1.4 644,776 + -2.0 644,776 + -0.6 644,776 + -2.8 644,776 + -2.6 644,776 + -0.5 644,777 - -0.0 644,777 - -0.0 644,777 - -0.0 644,828 + -1.8 644,972 + -0.6 644,972 + -1.2 644,972 + -1.6 644,972 + -0.6 644,972 + -0.0 644,972 + -1.0 644,972 + -1.7 644,973 - +0.3 644,973 - +0.1 645,003 + -1.8 645,004 - +1.4 645,140 + Pf1N1B4_547 0.49 -1.0 645,177 + Pf1N1B4_547 0.61 +1.0 645,177 + -1.0 645,177 + Pf1N1B4_547 0.61 -3.2 645,178 - Pf1N1B4_547 0.61 -1.0 645,178 - Pf1N1B4_547 0.61 -1.2 645,191 + Pf1N1B4_547 0.65 -1.4 645,192 - Pf1N1B4_547 0.65 -0.5 645,237 - Pf1N1B4_547 0.79 +0.3 645,291 + -0.5 645,291 + -1.3 645,292 - -0.7 645,292 - +1.4 645,292 - +1.0 645,292 - +0.2 645,337 - +1.3 645,337 - +3.7 645,337 - +0.3 645,397 - +1.0 645,397 - +1.5 645,397 - -0.2 645,397 - +0.0 645,470 + Pf1N1B4_548 0.12 -0.0 645,470 + Pf1N1B4_548 0.12 +1.0 645,471 - +1.0 645,471 - Pf1N1B4_548 0.12 +0.4 645,513 + Pf1N1B4_548 0.17 +1.2 646,122 + Pf1N1B4_548 0.83 +3.1 646,160 + Pf1N1B4_548 0.87 -0.6 646,160 + Pf1N1B4_548 0.87 +0.4 646,160 + Pf1N1B4_548 0.87 +1.0 646,161 - Pf1N1B4_548 0.87 +2.3 646,161 - Pf1N1B4_548 0.87 -0.6 646,161 - Pf1N1B4_548 0.87 +1.0 646,161 - Pf1N1B4_548 0.87 -1.6 646,161 - Pf1N1B4_548 0.87 -0.0 646,161 - Pf1N1B4_548 0.87 +0.4 646,161 - Pf1N1B4_548 0.87 +1.0 646,166 + Pf1N1B4_548 0.88 +2.0 646,289 + +1.0 646,289 + +1.4 646,289 + +1.2 646,289 + +0.0 646,289 + +0.4 646,289 + +1.5 646,289 + -1.6 646,289 + +1.0 646,289 + +1.0 646,289 + +1.4 646,289 + -0.8 646,289 + +2.5 646,290 - -0.5 646,290 - +1.7 646,290 - -0.0 646,290 - +0.5 646,290 - -2.4 646,290 - -0.0 646,290 - +0.1 646,290 - -1.4 646,290 - +0.2 646,290 - +2.8 646,290 - -0.8 646,290 - -1.4 646,290 - -1.0 646,290 - +0.3 646,293 + +1.3 646,293 + +0.6 646,293 + -1.0 646,293 + -0.0 646,293 + +0.5 646,293 + +0.8 646,293 + +0.5 646,294 - +1.5 646,294 - +0.6 646,294 - +0.2 646,294 - -0.5
Or see this region's nucleotide sequence