Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5448 and Pf1N1B4_5449 are separated by 312 nucleotides Pf1N1B4_5449 and Pf1N1B4_5450 overlap by 25 nucleotides
Pf1N1B4_5448 - hypothetical protein, at 194,385 to 194,507
_5448
Pf1N1B4_5449 - Error-prone repair protein UmuD, at 194,820 to 195,131
_5449
Pf1N1B4_5450 - Error-prone, lesion bypass DNA polymerase V (UmuC), at 195,107 to 196,402
_5450
Position (kb) 195
196
197 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 194.176 kb on + strand at 194.176 kb on + strand at 194.177 kb on - strand at 194.177 kb on - strand at 194.177 kb on - strand at 194.177 kb on - strand at 194.278 kb on - strand at 194.278 kb on - strand at 194.328 kb on + strand at 194.328 kb on + strand at 194.329 kb on - strand at 194.440 kb on + strand, within Pf1N1B4_5448 at 194.441 kb on - strand, within Pf1N1B4_5448 at 194.441 kb on - strand, within Pf1N1B4_5448 at 194.441 kb on - strand, within Pf1N1B4_5448 at 194.441 kb on - strand at 194.441 kb on - strand, within Pf1N1B4_5448 at 194.495 kb on + strand at 194.496 kb on - strand at 194.543 kb on - strand at 194.543 kb on - strand at 194.543 kb on - strand at 194.601 kb on + strand at 194.602 kb on - strand at 194.672 kb on - strand at 194.709 kb on - strand at 194.709 kb on - strand at 194.730 kb on + strand at 194.731 kb on - strand at 194.731 kb on - strand at 194.731 kb on - strand at 194.749 kb on + strand at 194.750 kb on - strand at 194.798 kb on + strand at 194.798 kb on + strand at 194.799 kb on - strand at 194.799 kb on - strand at 195.050 kb on + strand, within Pf1N1B4_5449 at 195.051 kb on - strand, within Pf1N1B4_5449 at 195.173 kb on + strand at 195.174 kb on - strand at 195.282 kb on + strand, within Pf1N1B4_5450 at 195.282 kb on + strand, within Pf1N1B4_5450 at 195.345 kb on + strand at 195.345 kb on + strand, within Pf1N1B4_5450 at 195.345 kb on + strand, within Pf1N1B4_5450 at 195.345 kb on + strand, within Pf1N1B4_5450 at 195.346 kb on - strand, within Pf1N1B4_5450 at 195.346 kb on - strand, within Pf1N1B4_5450 at 195.346 kb on - strand, within Pf1N1B4_5450 at 195.349 kb on - strand, within Pf1N1B4_5450 at 195.349 kb on - strand, within Pf1N1B4_5450 at 195.379 kb on + strand, within Pf1N1B4_5450 at 195.441 kb on + strand, within Pf1N1B4_5450 at 195.441 kb on + strand, within Pf1N1B4_5450 at 195.441 kb on + strand, within Pf1N1B4_5450 at 195.442 kb on - strand, within Pf1N1B4_5450 at 195.442 kb on - strand, within Pf1N1B4_5450 at 195.456 kb on + strand, within Pf1N1B4_5450 at 195.456 kb on + strand, within Pf1N1B4_5450 at 195.456 kb on + strand, within Pf1N1B4_5450 at 195.456 kb on + strand, within Pf1N1B4_5450 at 195.456 kb on + strand, within Pf1N1B4_5450 at 195.457 kb on - strand, within Pf1N1B4_5450 at 195.457 kb on - strand, within Pf1N1B4_5450 at 195.457 kb on - strand, within Pf1N1B4_5450 at 195.550 kb on + strand, within Pf1N1B4_5450 at 195.633 kb on + strand, within Pf1N1B4_5450 at 195.633 kb on + strand, within Pf1N1B4_5450 at 195.633 kb on + strand, within Pf1N1B4_5450 at 195.634 kb on - strand, within Pf1N1B4_5450 at 195.634 kb on - strand, within Pf1N1B4_5450 at 195.634 kb on - strand at 195.634 kb on - strand, within Pf1N1B4_5450 at 195.649 kb on + strand, within Pf1N1B4_5450 at 195.649 kb on + strand, within Pf1N1B4_5450 at 195.649 kb on + strand, within Pf1N1B4_5450 at 195.649 kb on + strand at 195.649 kb on + strand, within Pf1N1B4_5450 at 195.650 kb on - strand, within Pf1N1B4_5450 at 195.650 kb on - strand, within Pf1N1B4_5450 at 195.669 kb on - strand, within Pf1N1B4_5450 at 195.774 kb on + strand, within Pf1N1B4_5450 at 195.774 kb on + strand, within Pf1N1B4_5450 at 195.884 kb on - strand, within Pf1N1B4_5450 at 195.884 kb on - strand, within Pf1N1B4_5450 at 195.915 kb on + strand, within Pf1N1B4_5450 at 196.022 kb on + strand, within Pf1N1B4_5450 at 196.023 kb on - strand, within Pf1N1B4_5450 at 196.052 kb on - strand, within Pf1N1B4_5450 at 196.063 kb on + strand, within Pf1N1B4_5450 at 196.068 kb on + strand, within Pf1N1B4_5450 at 196.117 kb on + strand, within Pf1N1B4_5450 at 196.118 kb on - strand, within Pf1N1B4_5450 at 196.122 kb on + strand, within Pf1N1B4_5450 at 196.122 kb on + strand, within Pf1N1B4_5450 at 196.123 kb on - strand, within Pf1N1B4_5450 at 196.123 kb on - strand, within Pf1N1B4_5450 at 196.171 kb on - strand, within Pf1N1B4_5450 at 196.171 kb on - strand, within Pf1N1B4_5450 at 196.263 kb on + strand, within Pf1N1B4_5450 at 196.264 kb on - strand, within Pf1N1B4_5450 at 196.386 kb on + strand at 196.386 kb on + strand at 196.389 kb on + strand at 196.389 kb on + strand at 196.396 kb on + strand at 196.396 kb on + strand at 196.397 kb on - strand at 196.426 kb on + strand at 196.426 kb on + strand at 196.426 kb on + strand at 196.441 kb on + strand at 196.441 kb on + strand at 196.451 kb on + strand at 196.452 kb on - strand at 196.478 kb on + strand at 196.479 kb on - strand at 196.479 kb on - strand at 196.523 kb on + strand at 196.641 kb on + strand at 196.641 kb on + strand at 196.641 kb on + strand at 196.641 kb on + strand at 196.641 kb on + strand at 196.694 kb on + strand at 196.694 kb on + strand at 196.695 kb on - strand at 196.695 kb on - strand at 196.695 kb on - strand at 196.737 kb on + strand at 196.737 kb on + strand at 196.737 kb on + strand at 196.738 kb on - strand at 196.748 kb on + strand at 196.748 kb on + strand at 196.750 kb on + strand at 196.750 kb on + strand at 196.750 kb on + strand at 196.750 kb on + strand at 196.768 kb on + strand at 196.768 kb on + strand at 196.768 kb on + strand at 196.768 kb on + strand at 196.768 kb on + strand at 196.768 kb on + strand at 196.768 kb on + strand at 196.835 kb on + strand at 196.835 kb on + strand at 196.835 kb on + strand at 196.836 kb on - strand at 196.876 kb on + strand at 197.012 kb on + strand at 197.012 kb on + strand at 197.013 kb on - strand at 197.039 kb on + strand at 197.040 kb on - strand at 197.154 kb on + strand at 197.162 kb on + strand at 197.162 kb on + strand at 197.163 kb on - strand at 197.241 kb on + strand at 197.241 kb on + strand at 197.241 kb on + strand at 197.241 kb on + strand at 197.241 kb on + strand at 197.242 kb on - strand at 197.310 kb on + strand at 197.310 kb on + strand at 197.310 kb on + strand at 197.310 kb on + strand at 197.310 kb on + strand at 197.311 kb on - strand at 197.311 kb on - strand at 197.311 kb on - strand at 197.311 kb on - strand at 197.339 kb on - strand at 197.339 kb on - strand at 197.339 kb on - strand at 197.342 kb on + strand at 197.342 kb on + strand at 197.342 kb on + strand at 197.342 kb on + strand at 197.342 kb on + strand at 197.343 kb on - strand at 197.343 kb on - strand at 197.343 kb on - strand at 197.343 kb on - strand at 197.343 kb on - strand at 197.343 kb on - strand at 197.343 kb on - strand at 197.343 kb on - strand at 197.378 kb on - strand at 197.378 kb on - strand at 197.388 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 194,176 + +1.8 194,176 + -0.4 194,177 - +0.9 194,177 - +0.2 194,177 - +0.2 194,177 - -0.1 194,278 - +1.8 194,278 - -0.2 194,328 + +0.2 194,328 + +1.6 194,329 - +0.6 194,440 + Pf1N1B4_5448 0.45 +0.8 194,441 - Pf1N1B4_5448 0.46 -0.6 194,441 - Pf1N1B4_5448 0.46 -0.6 194,441 - Pf1N1B4_5448 0.46 -0.4 194,441 - +0.2 194,441 - Pf1N1B4_5448 0.46 -0.0 194,495 + +2.2 194,496 - +1.5 194,543 - +0.5 194,543 - +0.2 194,543 - +1.3 194,601 + -0.1 194,602 - +1.5 194,672 - -1.8 194,709 - -0.8 194,709 - -1.4 194,730 + -1.4 194,731 - +0.2 194,731 - -2.8 194,731 - -1.4 194,749 + -1.4 194,750 - -1.4 194,798 + -1.8 194,798 + -2.8 194,799 - -0.8 194,799 - +0.2 195,050 + Pf1N1B4_5449 0.74 -3.9 195,051 - Pf1N1B4_5449 0.74 -1.8 195,173 + -0.8 195,174 - -1.8 195,282 + Pf1N1B4_5450 0.14 -2.6 195,282 + Pf1N1B4_5450 0.14 -0.8 195,345 + +0.2 195,345 + Pf1N1B4_5450 0.18 -2.6 195,345 + Pf1N1B4_5450 0.18 -0.8 195,345 + Pf1N1B4_5450 0.18 -1.4 195,346 - Pf1N1B4_5450 0.18 -1.7 195,346 - Pf1N1B4_5450 0.18 -0.6 195,346 - Pf1N1B4_5450 0.18 -1.4 195,349 - Pf1N1B4_5450 0.19 -0.8 195,349 - Pf1N1B4_5450 0.19 -1.4 195,379 + Pf1N1B4_5450 0.21 -1.4 195,441 + Pf1N1B4_5450 0.26 -1.6 195,441 + Pf1N1B4_5450 0.26 -1.2 195,441 + Pf1N1B4_5450 0.26 -0.8 195,442 - Pf1N1B4_5450 0.26 -1.4 195,442 - Pf1N1B4_5450 0.26 -0.8 195,456 + Pf1N1B4_5450 0.27 +0.4 195,456 + Pf1N1B4_5450 0.27 +1.8 195,456 + Pf1N1B4_5450 0.27 -0.8 195,456 + Pf1N1B4_5450 0.27 -2.8 195,456 + Pf1N1B4_5450 0.27 -0.8 195,457 - Pf1N1B4_5450 0.27 -1.1 195,457 - Pf1N1B4_5450 0.27 +1.4 195,457 - Pf1N1B4_5450 0.27 -1.8 195,550 + Pf1N1B4_5450 0.34 -2.4 195,633 + Pf1N1B4_5450 0.41 -1.4 195,633 + Pf1N1B4_5450 0.41 -2.1 195,633 + Pf1N1B4_5450 0.41 +0.2 195,634 - Pf1N1B4_5450 0.41 -1.8 195,634 - Pf1N1B4_5450 0.41 -2.4 195,634 - -0.4 195,634 - Pf1N1B4_5450 0.41 -1.8 195,649 + Pf1N1B4_5450 0.42 -0.3 195,649 + Pf1N1B4_5450 0.42 +0.2 195,649 + Pf1N1B4_5450 0.42 -0.8 195,649 + -0.4 195,649 + Pf1N1B4_5450 0.42 -0.1 195,650 - Pf1N1B4_5450 0.42 -1.4 195,650 - Pf1N1B4_5450 0.42 -2.4 195,669 - Pf1N1B4_5450 0.43 +1.8 195,774 + Pf1N1B4_5450 0.51 -2.1 195,774 + Pf1N1B4_5450 0.51 -0.8 195,884 - Pf1N1B4_5450 0.60 -2.6 195,884 - Pf1N1B4_5450 0.60 -1.1 195,915 + Pf1N1B4_5450 0.62 +0.8 196,022 + Pf1N1B4_5450 0.71 -0.5 196,023 - Pf1N1B4_5450 0.71 -1.8 196,052 - Pf1N1B4_5450 0.73 -2.8 196,063 + Pf1N1B4_5450 0.74 -1.8 196,068 + Pf1N1B4_5450 0.74 -1.8 196,117 + Pf1N1B4_5450 0.78 +0.2 196,118 - Pf1N1B4_5450 0.78 -0.4 196,122 + Pf1N1B4_5450 0.78 -1.8 196,122 + Pf1N1B4_5450 0.78 -0.1 196,123 - Pf1N1B4_5450 0.78 -2.6 196,123 - Pf1N1B4_5450 0.78 -1.8 196,171 - Pf1N1B4_5450 0.82 +0.5 196,171 - Pf1N1B4_5450 0.82 -1.4 196,263 + Pf1N1B4_5450 0.89 -1.4 196,264 - Pf1N1B4_5450 0.89 -2.6 196,386 + +0.2 196,386 + -2.8 196,389 + -0.6 196,389 + +0.2 196,396 + -0.4 196,396 + -0.6 196,397 - -2.4 196,426 + +0.2 196,426 + -0.7 196,426 + +0.2 196,441 + -2.4 196,441 + -0.1 196,451 + -0.6 196,452 - -2.1 196,478 + -0.8 196,479 - -1.9 196,479 - -3.4 196,523 + +0.2 196,641 + -0.8 196,641 + -1.7 196,641 + -2.8 196,641 + -0.2 196,641 + -2.4 196,694 + -0.6 196,694 + -0.5 196,695 - -3.0 196,695 - -0.6 196,695 - -1.8 196,737 + -1.8 196,737 + -1.1 196,737 + -2.1 196,738 - +2.5 196,748 + -0.2 196,748 + +1.2 196,750 + +0.2 196,750 + +0.2 196,750 + -0.8 196,750 + -2.1 196,768 + +1.2 196,768 + +1.8 196,768 + -0.8 196,768 + -0.8 196,768 + +0.8 196,768 + -1.6 196,768 + -3.1 196,835 + -1.4 196,835 + +0.6 196,835 + +0.8 196,836 - +1.5 196,876 + +0.2 197,012 + -1.0 197,012 + -0.4 197,013 - -0.6 197,039 + +0.2 197,040 - -1.8 197,154 + +0.9 197,162 + -0.7 197,162 + -0.1 197,163 - +0.7 197,241 + -2.4 197,241 + -0.2 197,241 + -1.2 197,241 + -0.4 197,241 + -0.1 197,242 - -0.3 197,310 + +1.9 197,310 + -0.4 197,310 + -0.6 197,310 + -3.0 197,310 + -1.1 197,311 - +1.1 197,311 - -0.8 197,311 - +1.0 197,311 - +0.5 197,339 - -0.4 197,339 - -1.0 197,339 - -2.4 197,342 + +1.5 197,342 + +0.5 197,342 + +0.0 197,342 + -2.4 197,342 + -2.1 197,343 - -2.0 197,343 - -2.6 197,343 - +1.3 197,343 - +0.0 197,343 - +1.5 197,343 - -0.1 197,343 - +0.2 197,343 - +0.2 197,378 - -0.1 197,378 - -2.1 197,388 + -0.4
Or see this region's nucleotide sequence