Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_525

Experiment: Cisplatin 0.006 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_524 and Pf1N1B4_525 are separated by 310 nucleotidesPf1N1B4_525 and Pf1N1B4_526 are separated by 9 nucleotidesPf1N1B4_526 and Pf1N1B4_527 are separated by 51 nucleotides Pf1N1B4_524 - Transcriptional regulator GbuR, at 623,365 to 624,258 _524 Pf1N1B4_525 - Agmatinase (EC 3.5.3.11), at 624,569 to 625,519 _525 Pf1N1B4_526 - Undecaprenyl pyrophosphate synthase, at 625,529 to 625,951 _526 Pf1N1B4_527 - Cytosine/purine/uracil/thiamine/allantoin permease family protein, at 626,003 to 627,514 _527 Position (kb) 624 625 626Strain fitness (log2 ratio) -2 -1 0 1 2 3at 623.586 kb on + strand, within Pf1N1B4_524at 623.586 kb on + strand, within Pf1N1B4_524at 623.586 kb on + strand, within Pf1N1B4_524at 623.586 kb on + strand, within Pf1N1B4_524at 623.587 kb on - strand, within Pf1N1B4_524at 623.694 kb on + strand, within Pf1N1B4_524at 623.695 kb on - strandat 623.765 kb on + strand, within Pf1N1B4_524at 623.765 kb on + strand, within Pf1N1B4_524at 623.765 kb on + strand, within Pf1N1B4_524at 623.766 kb on - strand, within Pf1N1B4_524at 623.766 kb on - strand, within Pf1N1B4_524at 623.846 kb on + strand, within Pf1N1B4_524at 623.846 kb on + strand, within Pf1N1B4_524at 623.897 kb on + strand, within Pf1N1B4_524at 624.002 kb on + strandat 624.002 kb on + strand, within Pf1N1B4_524at 624.002 kb on + strand, within Pf1N1B4_524at 624.003 kb on - strand, within Pf1N1B4_524at 624.140 kb on + strand, within Pf1N1B4_524at 624.140 kb on + strand, within Pf1N1B4_524at 624.140 kb on + strand, within Pf1N1B4_524at 624.141 kb on - strand, within Pf1N1B4_524at 624.141 kb on - strand, within Pf1N1B4_524at 624.141 kb on - strand, within Pf1N1B4_524at 624.141 kb on - strand, within Pf1N1B4_524at 624.229 kb on + strandat 624.229 kb on + strandat 624.277 kb on - strandat 624.277 kb on - strandat 624.399 kb on + strandat 624.400 kb on - strandat 624.408 kb on - strandat 624.445 kb on + strandat 624.445 kb on + strandat 624.446 kb on - strandat 624.479 kb on + strandat 624.479 kb on + strandat 624.538 kb on + strandat 624.538 kb on + strandat 624.538 kb on + strandat 624.538 kb on + strandat 624.539 kb on - strandat 624.539 kb on - strandat 624.542 kb on + strandat 624.542 kb on + strandat 624.543 kb on - strandat 624.779 kb on + strand, within Pf1N1B4_525at 624.780 kb on - strand, within Pf1N1B4_525at 624.780 kb on - strand, within Pf1N1B4_525at 624.894 kb on - strand, within Pf1N1B4_525at 624.979 kb on + strand, within Pf1N1B4_525at 624.980 kb on - strand, within Pf1N1B4_525at 625.086 kb on + strand, within Pf1N1B4_525at 625.087 kb on - strand, within Pf1N1B4_525at 625.145 kb on + strand, within Pf1N1B4_525at 625.145 kb on + strand, within Pf1N1B4_525at 625.146 kb on - strand, within Pf1N1B4_525at 625.274 kb on + strand, within Pf1N1B4_525at 625.274 kb on + strand, within Pf1N1B4_525at 625.434 kb on - strandat 625.479 kb on - strandat 625.604 kb on + strand, within Pf1N1B4_526at 625.605 kb on - strand, within Pf1N1B4_526at 625.605 kb on - strand, within Pf1N1B4_526at 625.643 kb on + strand, within Pf1N1B4_526at 625.644 kb on - strandat 625.644 kb on - strand, within Pf1N1B4_526at 625.679 kb on + strand, within Pf1N1B4_526at 625.679 kb on + strand, within Pf1N1B4_526at 625.893 kb on + strand, within Pf1N1B4_526at 625.893 kb on + strand, within Pf1N1B4_526at 626.009 kb on - strandat 626.032 kb on + strandat 626.033 kb on - strandat 626.456 kb on + strand, within Pf1N1B4_527at 626.456 kb on + strand, within Pf1N1B4_527at 626.495 kb on + strand, within Pf1N1B4_527at 626.495 kb on + strand, within Pf1N1B4_527at 626.495 kb on + strand, within Pf1N1B4_527at 626.495 kb on + strand, within Pf1N1B4_527at 626.495 kb on + strand, within Pf1N1B4_527

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml
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623,586 + Pf1N1B4_524 0.25 -1.3
623,586 + Pf1N1B4_524 0.25 +1.5
623,586 + Pf1N1B4_524 0.25 +1.8
623,586 + Pf1N1B4_524 0.25 +1.5
623,587 - Pf1N1B4_524 0.25 +0.5
623,694 + Pf1N1B4_524 0.37 -0.6
623,695 - +1.5
623,765 + Pf1N1B4_524 0.45 -1.6
623,765 + Pf1N1B4_524 0.45 +0.5
623,765 + Pf1N1B4_524 0.45 +0.4
623,766 - Pf1N1B4_524 0.45 -1.2
623,766 - Pf1N1B4_524 0.45 -0.3
623,846 + Pf1N1B4_524 0.54 +0.2
623,846 + Pf1N1B4_524 0.54 -0.0
623,897 + Pf1N1B4_524 0.60 +0.5
624,002 + +1.0
624,002 + Pf1N1B4_524 0.71 -1.6
624,002 + Pf1N1B4_524 0.71 +0.5
624,003 - Pf1N1B4_524 0.71 -0.0
624,140 + Pf1N1B4_524 0.87 -0.5
624,140 + Pf1N1B4_524 0.87 +0.5
624,140 + Pf1N1B4_524 0.87 +3.1
624,141 - Pf1N1B4_524 0.87 +0.2
624,141 - Pf1N1B4_524 0.87 +2.1
624,141 - Pf1N1B4_524 0.87 +1.0
624,141 - Pf1N1B4_524 0.87 +0.4
624,229 + +1.1
624,229 + -1.0
624,277 - -0.0
624,277 - -0.3
624,399 + +0.8
624,400 - -2.6
624,408 - +1.0
624,445 + -1.0
624,445 + +0.5
624,446 - +0.6
624,479 + -0.4
624,479 + -1.4
624,538 + +1.6
624,538 + +1.2
624,538 + +0.5
624,538 + +1.0
624,539 - -0.8
624,539 - -1.4
624,542 + -0.3
624,542 + -0.0
624,543 - +0.8
624,779 + Pf1N1B4_525 0.22 -1.6
624,780 - Pf1N1B4_525 0.22 -0.3
624,780 - Pf1N1B4_525 0.22 -2.2
624,894 - Pf1N1B4_525 0.34 -0.0
624,979 + Pf1N1B4_525 0.43 -1.0
624,980 - Pf1N1B4_525 0.43 -1.0
625,086 + Pf1N1B4_525 0.54 -1.3
625,087 - Pf1N1B4_525 0.54 -0.5
625,145 + Pf1N1B4_525 0.61 -1.0
625,145 + Pf1N1B4_525 0.61 +1.6
625,146 - Pf1N1B4_525 0.61 +0.4
625,274 + Pf1N1B4_525 0.74 -2.4
625,274 + Pf1N1B4_525 0.74 -1.0
625,434 - +0.0
625,479 - +2.0
625,604 + Pf1N1B4_526 0.18 +0.6
625,605 - Pf1N1B4_526 0.18 +2.3
625,605 - Pf1N1B4_526 0.18 -0.4
625,643 + Pf1N1B4_526 0.27 +0.7
625,644 - -0.0
625,644 - Pf1N1B4_526 0.27 -0.0
625,679 + Pf1N1B4_526 0.35 +1.0
625,679 + Pf1N1B4_526 0.35 -0.0
625,893 + Pf1N1B4_526 0.86 -1.0
625,893 + Pf1N1B4_526 0.86 +1.0
626,009 - +1.0
626,032 + -0.0
626,033 - +0.4
626,456 + Pf1N1B4_527 0.30 +0.6
626,456 + Pf1N1B4_527 0.30 +1.0
626,495 + Pf1N1B4_527 0.33 -0.4
626,495 + Pf1N1B4_527 0.33 +0.4
626,495 + Pf1N1B4_527 0.33 +0.2
626,495 + Pf1N1B4_527 0.33 +0.3
626,495 + Pf1N1B4_527 0.33 -0.5

Or see this region's nucleotide sequence