Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4608 and Pf1N1B4_4609 overlap by 1 nucleotides Pf1N1B4_4609 and Pf1N1B4_4610 overlap by 31 nucleotides Pf1N1B4_4610 and Pf1N1B4_4611 overlap by 4 nucleotides Pf1N1B4_4611 and Pf1N1B4_4612 are separated by 30 nucleotides Pf1N1B4_4612 and Pf1N1B4_4613 overlap by 4 nucleotides
Pf1N1B4_4608 - NAD kinase (EC 2.7.1.23), at 5,079,704 to 5,079,934
_4608
Pf1N1B4_4609 - Diadenosine tetraphosphatase and related serine/threonine protein phosphatases, at 5,079,934 to 5,080,398
_4609
Pf1N1B4_4610 - Diadenosine tetraphosphatase and related serine/threonine protein phosphatases, at 5,080,368 to 5,080,904
_4610
Pf1N1B4_4611 - GlpG protein (membrane protein of glp regulon), at 5,080,901 to 5,081,773
_4611
Pf1N1B4_4612 - FIG00954117: hypothetical protein, at 5,081,804 to 5,082,064
_4612
Pf1N1B4_4613 - FIG00953934: hypothetical protein, at 5,082,061 to 5,082,891
_4613
Position (kb) 5080
5081
5082 Strain fitness (log2 ratio) -2
-1
0
1 at 5079.951 kb on + strand at 5079.956 kb on - strand at 5080.243 kb on - strand, within Pf1N1B4_4609 at 5080.329 kb on + strand, within Pf1N1B4_4609 at 5080.701 kb on + strand, within Pf1N1B4_4610 at 5080.737 kb on + strand, within Pf1N1B4_4610 at 5080.859 kb on + strand at 5080.920 kb on + strand at 5080.920 kb on + strand at 5080.920 kb on + strand at 5080.920 kb on + strand at 5080.921 kb on - strand at 5081.019 kb on + strand, within Pf1N1B4_4611 at 5081.136 kb on + strand, within Pf1N1B4_4611 at 5081.477 kb on + strand, within Pf1N1B4_4611 at 5081.477 kb on + strand, within Pf1N1B4_4611 at 5081.477 kb on + strand, within Pf1N1B4_4611 at 5081.477 kb on + strand, within Pf1N1B4_4611 at 5081.477 kb on + strand, within Pf1N1B4_4611 at 5081.744 kb on + strand at 5081.744 kb on + strand at 5081.745 kb on - strand at 5081.772 kb on - strand at 5081.776 kb on + strand at 5081.776 kb on + strand at 5081.777 kb on - strand at 5081.777 kb on - strand at 5081.777 kb on - strand at 5081.849 kb on + strand, within Pf1N1B4_4612 at 5081.849 kb on + strand, within Pf1N1B4_4612 at 5081.850 kb on - strand, within Pf1N1B4_4612 at 5081.850 kb on - strand, within Pf1N1B4_4612 at 5081.850 kb on - strand, within Pf1N1B4_4612 at 5081.987 kb on + strand at 5081.988 kb on - strand, within Pf1N1B4_4612 at 5082.197 kb on - strand, within Pf1N1B4_4613 at 5082.329 kb on - strand, within Pf1N1B4_4613 at 5082.329 kb on - strand, within Pf1N1B4_4613 at 5082.578 kb on + strand, within Pf1N1B4_4613
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 5,079,951 + -0.2 5,079,956 - -0.7 5,080,243 - Pf1N1B4_4609 0.66 +1.2 5,080,329 + Pf1N1B4_4609 0.85 -1.0 5,080,701 + Pf1N1B4_4610 0.62 +0.3 5,080,737 + Pf1N1B4_4610 0.69 -0.9 5,080,859 + +0.9 5,080,920 + +0.6 5,080,920 + +1.6 5,080,920 + -0.2 5,080,920 + -1.4 5,080,921 - +0.6 5,081,019 + Pf1N1B4_4611 0.14 -0.7 5,081,136 + Pf1N1B4_4611 0.27 -2.0 5,081,477 + Pf1N1B4_4611 0.66 -2.0 5,081,477 + Pf1N1B4_4611 0.66 -2.0 5,081,477 + Pf1N1B4_4611 0.66 -0.0 5,081,477 + Pf1N1B4_4611 0.66 -1.4 5,081,477 + Pf1N1B4_4611 0.66 -0.0 5,081,744 + +1.0 5,081,744 + -0.9 5,081,745 - -0.4 5,081,772 - +0.9 5,081,776 + +0.9 5,081,776 + -0.4 5,081,777 - +0.6 5,081,777 - -0.8 5,081,777 - +0.6 5,081,849 + Pf1N1B4_4612 0.17 -1.2 5,081,849 + Pf1N1B4_4612 0.17 -1.4 5,081,850 - Pf1N1B4_4612 0.18 -0.4 5,081,850 - Pf1N1B4_4612 0.18 +0.9 5,081,850 - Pf1N1B4_4612 0.18 -0.4 5,081,987 + -0.4 5,081,988 - Pf1N1B4_4612 0.70 -1.2 5,082,197 - Pf1N1B4_4613 0.16 -0.5 5,082,329 - Pf1N1B4_4613 0.32 -0.1 5,082,329 - Pf1N1B4_4613 0.32 -1.0 5,082,578 + Pf1N1B4_4613 0.62 -1.8
Or see this region's nucleotide sequence