Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4542 and Pf1N1B4_4543 are separated by 2 nucleotides Pf1N1B4_4543 and Pf1N1B4_4544 are separated by 502 nucleotides Pf1N1B4_4544 and Pf1N1B4_4545 are separated by 88 nucleotides Pf1N1B4_4545 and Pf1N1B4_4546 overlap by 2 nucleotides
Pf1N1B4_4542 - IcmF-related protein, at 5,009,265 to 5,012,888
_4542
Pf1N1B4_4543 - IcmF-related protein, at 5,012,891 to 5,013,040
_4543
Pf1N1B4_4544 - Uncharacterized protein ImpA, at 5,013,543 to 5,014,181
_4544
Pf1N1B4_4545 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 5,014,270 to 5,014,758
_4545
Pf1N1B4_4546 - hypothetical protein, at 5,014,757 to 5,015,215
_4546
Position (kb) 5013
5014
5015 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5012.799 kb on + strand at 5012.821 kb on + strand at 5012.872 kb on - strand at 5013.790 kb on - strand, within Pf1N1B4_4544 at 5014.030 kb on - strand, within Pf1N1B4_4544 at 5014.065 kb on + strand, within Pf1N1B4_4544 at 5014.179 kb on + strand at 5015.115 kb on + strand, within Pf1N1B4_4546
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 5,012,799 + +0.2 5,012,821 + +0.2 5,012,872 - -0.4 5,013,790 - Pf1N1B4_4544 0.39 -0.7 5,014,030 - Pf1N1B4_4544 0.76 -3.7 5,014,065 + Pf1N1B4_4544 0.82 -0.8 5,014,179 + -0.2 5,015,115 + Pf1N1B4_4546 0.78 -0.7
Or see this region's nucleotide sequence