Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4425

Experiment: Cisplatin 0.006 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4424 and Pf1N1B4_4425 overlap by 4 nucleotidesPf1N1B4_4425 and Pf1N1B4_4426 overlap by 4 nucleotidesPf1N1B4_4426 and Pf1N1B4_4427 overlap by 11 nucleotides Pf1N1B4_4424 - D-2-hydroxyglutarate dehydrogenase, at 4,884,343 to 4,885,767 _4424 Pf1N1B4_4425 - Aspartate aminotransferase (EC 2.6.1.1), at 4,885,764 to 4,886,975 _4425 Pf1N1B4_4426 - Glutamate transport membrane-spanning protein, at 4,886,972 to 4,887,706 _4426 Pf1N1B4_4427 - Glutamate transport membrane-spanning protein, at 4,887,696 to 4,888,484 _4427 Position (kb) 4885 4886 4887Strain fitness (log2 ratio) -2 -1 0 1 2at 4885.471 kb on + strand, within Pf1N1B4_4424at 4885.472 kb on - strand, within Pf1N1B4_4424at 4885.507 kb on + strand, within Pf1N1B4_4424at 4885.507 kb on + strandat 4885.508 kb on - strand, within Pf1N1B4_4424at 4885.665 kb on + strandat 4885.666 kb on - strandat 4885.666 kb on - strandat 4885.666 kb on - strandat 4885.745 kb on + strandat 4885.926 kb on + strand, within Pf1N1B4_4425at 4885.956 kb on + strand, within Pf1N1B4_4425at 4886.032 kb on - strand, within Pf1N1B4_4425at 4886.244 kb on + strandat 4886.244 kb on + strand, within Pf1N1B4_4425at 4886.245 kb on - strand, within Pf1N1B4_4425at 4886.245 kb on - strand, within Pf1N1B4_4425at 4886.305 kb on + strandat 4886.308 kb on - strand, within Pf1N1B4_4425at 4886.308 kb on - strand, within Pf1N1B4_4425at 4886.487 kb on + strand, within Pf1N1B4_4425at 4886.488 kb on - strand, within Pf1N1B4_4425at 4886.678 kb on - strand, within Pf1N1B4_4425at 4886.744 kb on - strand, within Pf1N1B4_4425at 4886.748 kb on - strand, within Pf1N1B4_4425at 4887.026 kb on + strandat 4887.558 kb on - strand, within Pf1N1B4_4426

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml
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4,885,471 + Pf1N1B4_4424 0.79 +0.4
4,885,472 - Pf1N1B4_4424 0.79 -0.4
4,885,507 + Pf1N1B4_4424 0.82 -1.0
4,885,507 + +0.6
4,885,508 - Pf1N1B4_4424 0.82 -1.1
4,885,665 + +2.2
4,885,666 - -0.4
4,885,666 - -0.4
4,885,666 - +0.2
4,885,745 + -0.1
4,885,926 + Pf1N1B4_4425 0.13 -2.0
4,885,956 + Pf1N1B4_4425 0.16 -0.4
4,886,032 - Pf1N1B4_4425 0.22 +0.5
4,886,244 + -1.4
4,886,244 + Pf1N1B4_4425 0.40 -2.7
4,886,245 - Pf1N1B4_4425 0.40 -2.4
4,886,245 - Pf1N1B4_4425 0.40 +0.4
4,886,305 + +0.2
4,886,308 - Pf1N1B4_4425 0.45 -0.4
4,886,308 - Pf1N1B4_4425 0.45 -0.6
4,886,487 + Pf1N1B4_4425 0.60 -1.7
4,886,488 - Pf1N1B4_4425 0.60 -0.8
4,886,678 - Pf1N1B4_4425 0.75 -1.4
4,886,744 - Pf1N1B4_4425 0.81 +1.2
4,886,748 - Pf1N1B4_4425 0.81 -0.4
4,887,026 + +0.1
4,887,558 - Pf1N1B4_4426 0.80 +0.6

Or see this region's nucleotide sequence