Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4421 and Pf1N1B4_4422 are separated by 252 nucleotides Pf1N1B4_4422 and Pf1N1B4_4423 are separated by 64 nucleotides Pf1N1B4_4423 and Pf1N1B4_4424 overlap by 4 nucleotides
Pf1N1B4_4421 - LysR family transcriptional regulator lrhA, at 4,881,475 to 4,882,335
_4421
Pf1N1B4_4422 - ABC transporter amino acid-binding protein, at 4,882,588 to 4,883,430
_4422
Pf1N1B4_4423 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 4,883,495 to 4,884,346
_4423
Pf1N1B4_4424 - D-2-hydroxyglutarate dehydrogenase, at 4,884,343 to 4,885,767
_4424
Position (kb)
4882
4883
4884 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4881.698 kb on + strand, within Pf1N1B4_4421 at 4881.698 kb on + strand, within Pf1N1B4_4421 at 4882.049 kb on - strand, within Pf1N1B4_4421 at 4882.049 kb on - strand, within Pf1N1B4_4421 at 4882.304 kb on - strand at 4882.357 kb on + strand at 4882.358 kb on - strand at 4882.730 kb on + strand, within Pf1N1B4_4422 at 4882.731 kb on - strand, within Pf1N1B4_4422 at 4882.967 kb on - strand, within Pf1N1B4_4422 at 4883.066 kb on - strand, within Pf1N1B4_4422 at 4883.850 kb on - strand, within Pf1N1B4_4423 at 4884.091 kb on + strand, within Pf1N1B4_4423 at 4884.091 kb on + strand, within Pf1N1B4_4423 at 4884.091 kb on + strand, within Pf1N1B4_4423 at 4884.092 kb on - strand, within Pf1N1B4_4423 at 4884.092 kb on - strand, within Pf1N1B4_4423 at 4884.092 kb on - strand, within Pf1N1B4_4423 at 4884.092 kb on - strand, within Pf1N1B4_4423 at 4884.092 kb on - strand, within Pf1N1B4_4423
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 4,881,698 + Pf1N1B4_4421 0.26 -0.4 4,881,698 + Pf1N1B4_4421 0.26 +1.9 4,882,049 - Pf1N1B4_4421 0.67 +0.6 4,882,049 - Pf1N1B4_4421 0.67 -1.1 4,882,304 - -0.1 4,882,357 + +0.8 4,882,358 - -1.7 4,882,730 + Pf1N1B4_4422 0.17 +0.2 4,882,731 - Pf1N1B4_4422 0.17 +0.6 4,882,967 - Pf1N1B4_4422 0.45 +0.8 4,883,066 - Pf1N1B4_4422 0.57 +2.2 4,883,850 - Pf1N1B4_4423 0.42 -0.4 4,884,091 + Pf1N1B4_4423 0.70 +0.8 4,884,091 + Pf1N1B4_4423 0.70 -2.7 4,884,091 + Pf1N1B4_4423 0.70 +0.6 4,884,092 - Pf1N1B4_4423 0.70 -0.8 4,884,092 - Pf1N1B4_4423 0.70 +0.5 4,884,092 - Pf1N1B4_4423 0.70 +0.8 4,884,092 - Pf1N1B4_4423 0.70 +0.1 4,884,092 - Pf1N1B4_4423 0.70 -0.7
Or see this region's nucleotide sequence