Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4279 and Pf1N1B4_4280 are separated by 32 nucleotides Pf1N1B4_4280 and Pf1N1B4_4281 are separated by 153 nucleotides
Pf1N1B4_4279 - 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase IolD (EC 3.7.1.22) (from data) , at 4,744,754 to 4,746,685
_4279
Pf1N1B4_4280 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 4,746,718 to 4,747,743
_4280
Pf1N1B4_4281 - Glyceraldehyde-3-phosphate ketol-isomerase (EC 5.3.1.1), at 4,747,897 to 4,748,757
_4281
Position (kb)
4746
4747
4748 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4745.821 kb on - strand, within Pf1N1B4_4279 at 4745.821 kb on - strand at 4745.901 kb on - strand, within Pf1N1B4_4279 at 4746.125 kb on + strand, within Pf1N1B4_4279 at 4746.125 kb on + strand at 4746.212 kb on + strand, within Pf1N1B4_4279 at 4746.212 kb on + strand, within Pf1N1B4_4279 at 4746.213 kb on - strand, within Pf1N1B4_4279 at 4746.236 kb on + strand, within Pf1N1B4_4279 at 4746.236 kb on + strand, within Pf1N1B4_4279 at 4746.236 kb on + strand, within Pf1N1B4_4279 at 4746.452 kb on + strand, within Pf1N1B4_4279 at 4746.467 kb on + strand, within Pf1N1B4_4279 at 4746.492 kb on + strand at 4746.578 kb on + strand at 4746.579 kb on - strand at 4746.715 kb on + strand at 4746.715 kb on + strand at 4746.716 kb on - strand at 4746.716 kb on - strand at 4746.716 kb on - strand at 4746.716 kb on - strand at 4746.717 kb on + strand at 4746.718 kb on - strand at 4746.718 kb on - strand at 4747.211 kb on + strand, within Pf1N1B4_4280 at 4747.222 kb on + strand, within Pf1N1B4_4280 at 4747.297 kb on + strand, within Pf1N1B4_4280 at 4747.297 kb on + strand, within Pf1N1B4_4280 at 4747.297 kb on + strand, within Pf1N1B4_4280 at 4747.297 kb on + strand, within Pf1N1B4_4280 at 4747.298 kb on - strand, within Pf1N1B4_4280 at 4747.298 kb on - strand, within Pf1N1B4_4280 at 4747.298 kb on - strand, within Pf1N1B4_4280 at 4747.703 kb on + strand at 4747.736 kb on - strand at 4747.919 kb on - strand at 4747.924 kb on + strand at 4747.925 kb on - strand at 4748.020 kb on + strand, within Pf1N1B4_4281 at 4748.020 kb on + strand, within Pf1N1B4_4281 at 4748.021 kb on - strand, within Pf1N1B4_4281 at 4748.021 kb on - strand, within Pf1N1B4_4281 at 4748.021 kb on - strand, within Pf1N1B4_4281 at 4748.102 kb on + strand, within Pf1N1B4_4281 at 4748.103 kb on - strand, within Pf1N1B4_4281 at 4748.113 kb on + strand, within Pf1N1B4_4281 at 4748.114 kb on - strand, within Pf1N1B4_4281 at 4748.114 kb on - strand, within Pf1N1B4_4281 at 4748.114 kb on - strand, within Pf1N1B4_4281 at 4748.339 kb on - strand, within Pf1N1B4_4281 at 4748.408 kb on - strand, within Pf1N1B4_4281 at 4748.437 kb on + strand, within Pf1N1B4_4281 at 4748.477 kb on - strand, within Pf1N1B4_4281 at 4748.477 kb on - strand, within Pf1N1B4_4281 at 4748.518 kb on + strand, within Pf1N1B4_4281 at 4748.518 kb on + strand, within Pf1N1B4_4281 at 4748.518 kb on + strand, within Pf1N1B4_4281 at 4748.519 kb on - strand, within Pf1N1B4_4281
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 4,745,821 - Pf1N1B4_4279 0.55 +0.7 4,745,821 - -1.9 4,745,901 - Pf1N1B4_4279 0.59 -1.3 4,746,125 + Pf1N1B4_4279 0.71 +2.0 4,746,125 + -1.3 4,746,212 + Pf1N1B4_4279 0.75 +0.2 4,746,212 + Pf1N1B4_4279 0.75 +1.7 4,746,213 - Pf1N1B4_4279 0.76 -1.2 4,746,236 + Pf1N1B4_4279 0.77 -1.2 4,746,236 + Pf1N1B4_4279 0.77 -3.1 4,746,236 + Pf1N1B4_4279 0.77 -0.6 4,746,452 + Pf1N1B4_4279 0.88 +1.2 4,746,467 + Pf1N1B4_4279 0.89 +3.1 4,746,492 + -0.7 4,746,578 + -0.1 4,746,579 - +0.2 4,746,715 + -1.3 4,746,715 + +0.4 4,746,716 - +0.4 4,746,716 - -3.1 4,746,716 - -1.9 4,746,716 - -0.6 4,746,717 + -1.3 4,746,718 - +0.7 4,746,718 - -1.9 4,747,211 + Pf1N1B4_4280 0.48 -1.7 4,747,222 + Pf1N1B4_4280 0.49 +2.0 4,747,297 + Pf1N1B4_4280 0.56 +2.7 4,747,297 + Pf1N1B4_4280 0.56 +2.1 4,747,297 + Pf1N1B4_4280 0.56 +2.0 4,747,297 + Pf1N1B4_4280 0.56 -0.3 4,747,298 - Pf1N1B4_4280 0.57 -0.2 4,747,298 - Pf1N1B4_4280 0.57 -0.1 4,747,298 - Pf1N1B4_4280 0.57 +1.0 4,747,703 + +0.3 4,747,736 - +2.0 4,747,919 - +2.5 4,747,924 + +0.1 4,747,925 - -1.6 4,748,020 + Pf1N1B4_4281 0.14 +0.5 4,748,020 + Pf1N1B4_4281 0.14 -0.3 4,748,021 - Pf1N1B4_4281 0.14 +0.6 4,748,021 - Pf1N1B4_4281 0.14 +0.9 4,748,021 - Pf1N1B4_4281 0.14 -0.9 4,748,102 + Pf1N1B4_4281 0.24 -0.6 4,748,103 - Pf1N1B4_4281 0.24 -0.6 4,748,113 + Pf1N1B4_4281 0.25 +1.7 4,748,114 - Pf1N1B4_4281 0.25 +0.9 4,748,114 - Pf1N1B4_4281 0.25 -0.3 4,748,114 - Pf1N1B4_4281 0.25 -0.9 4,748,339 - Pf1N1B4_4281 0.51 -0.3 4,748,408 - Pf1N1B4_4281 0.59 -0.3 4,748,437 + Pf1N1B4_4281 0.63 +0.5 4,748,477 - Pf1N1B4_4281 0.67 +0.7 4,748,477 - Pf1N1B4_4281 0.67 -1.5 4,748,518 + Pf1N1B4_4281 0.72 +1.0 4,748,518 + Pf1N1B4_4281 0.72 +0.9 4,748,518 + Pf1N1B4_4281 0.72 +1.7 4,748,519 - Pf1N1B4_4281 0.72 -0.3
Or see this region's nucleotide sequence