Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4241 and Pf1N1B4_4242 are separated by 225 nucleotides Pf1N1B4_4242 and Pf1N1B4_4243 are separated by 157 nucleotides
Pf1N1B4_4241 - Dienelactone hydrolase family, at 4,699,537 to 4,700,424
_4241
Pf1N1B4_4242 - cell processes; transport of small molecules; amino acids, amines, peptides, at 4,700,650 to 4,702,215
_4242
Pf1N1B4_4243 - Alcohol dehydrogenase (EC 1.1.1.1), at 4,702,373 to 4,703,533
_4243
Position (kb)
4700
4701
4702
4703 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4699.653 kb on + strand, within Pf1N1B4_4241 at 4699.653 kb on + strand, within Pf1N1B4_4241 at 4699.653 kb on + strand, within Pf1N1B4_4241 at 4699.655 kb on + strand, within Pf1N1B4_4241 at 4699.655 kb on + strand at 4699.655 kb on + strand, within Pf1N1B4_4241 at 4699.655 kb on + strand, within Pf1N1B4_4241 at 4699.656 kb on - strand, within Pf1N1B4_4241 at 4699.664 kb on + strand, within Pf1N1B4_4241 at 4699.664 kb on + strand, within Pf1N1B4_4241 at 4699.664 kb on + strand, within Pf1N1B4_4241 at 4699.665 kb on - strand, within Pf1N1B4_4241 at 4699.665 kb on - strand, within Pf1N1B4_4241 at 4699.665 kb on - strand, within Pf1N1B4_4241 at 4699.799 kb on + strand, within Pf1N1B4_4241 at 4699.799 kb on + strand, within Pf1N1B4_4241 at 4699.819 kb on + strand, within Pf1N1B4_4241 at 4700.087 kb on + strand, within Pf1N1B4_4241 at 4700.088 kb on - strand, within Pf1N1B4_4241 at 4700.201 kb on + strand, within Pf1N1B4_4241 at 4700.210 kb on + strand, within Pf1N1B4_4241 at 4700.210 kb on + strand, within Pf1N1B4_4241 at 4700.210 kb on + strand, within Pf1N1B4_4241 at 4700.211 kb on - strand, within Pf1N1B4_4241 at 4700.261 kb on + strand, within Pf1N1B4_4241 at 4700.262 kb on - strand, within Pf1N1B4_4241 at 4700.262 kb on - strand, within Pf1N1B4_4241 at 4700.367 kb on - strand at 4700.369 kb on + strand at 4700.369 kb on + strand at 4700.370 kb on - strand at 4700.375 kb on + strand at 4700.487 kb on + strand at 4700.487 kb on + strand at 4700.488 kb on - strand at 4700.546 kb on + strand at 4700.601 kb on + strand at 4700.889 kb on - strand at 4700.889 kb on - strand, within Pf1N1B4_4242 at 4700.889 kb on - strand, within Pf1N1B4_4242 at 4700.943 kb on - strand, within Pf1N1B4_4242 at 4700.946 kb on + strand, within Pf1N1B4_4242 at 4700.947 kb on - strand, within Pf1N1B4_4242 at 4700.947 kb on - strand, within Pf1N1B4_4242 at 4701.021 kb on - strand, within Pf1N1B4_4242 at 4701.453 kb on + strand, within Pf1N1B4_4242 at 4701.454 kb on - strand at 4701.486 kb on - strand at 4701.486 kb on - strand, within Pf1N1B4_4242 at 4701.567 kb on - strand, within Pf1N1B4_4242 at 4701.809 kb on + strand, within Pf1N1B4_4242 at 4701.812 kb on + strand, within Pf1N1B4_4242 at 4702.204 kb on + strand at 4702.215 kb on + strand at 4702.298 kb on + strand at 4702.299 kb on - strand at 4702.374 kb on + strand at 4702.522 kb on + strand, within Pf1N1B4_4243 at 4702.602 kb on + strand, within Pf1N1B4_4243 at 4702.655 kb on + strand at 4702.655 kb on + strand, within Pf1N1B4_4243 at 4702.655 kb on + strand, within Pf1N1B4_4243 at 4702.655 kb on + strand, within Pf1N1B4_4243 at 4702.656 kb on - strand, within Pf1N1B4_4243 at 4702.656 kb on - strand, within Pf1N1B4_4243 at 4702.659 kb on + strand at 4702.660 kb on - strand at 4702.701 kb on + strand at 4702.832 kb on + strand, within Pf1N1B4_4243 at 4702.873 kb on + strand, within Pf1N1B4_4243 at 4702.873 kb on + strand, within Pf1N1B4_4243 at 4702.874 kb on - strand, within Pf1N1B4_4243
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 4,699,653 + Pf1N1B4_4241 0.13 -1.3 4,699,653 + Pf1N1B4_4241 0.13 +1.0 4,699,653 + Pf1N1B4_4241 0.13 -1.3 4,699,655 + Pf1N1B4_4241 0.13 -0.1 4,699,655 + +1.7 4,699,655 + Pf1N1B4_4241 0.13 -1.3 4,699,655 + Pf1N1B4_4241 0.13 -0.3 4,699,656 - Pf1N1B4_4241 0.13 +0.3 4,699,664 + Pf1N1B4_4241 0.14 +1.0 4,699,664 + Pf1N1B4_4241 0.14 -2.3 4,699,664 + Pf1N1B4_4241 0.14 -0.3 4,699,665 - Pf1N1B4_4241 0.14 +0.4 4,699,665 - Pf1N1B4_4241 0.14 +0.6 4,699,665 - Pf1N1B4_4241 0.14 -0.3 4,699,799 + Pf1N1B4_4241 0.30 +0.2 4,699,799 + Pf1N1B4_4241 0.30 +2.0 4,699,819 + Pf1N1B4_4241 0.32 +1.3 4,700,087 + Pf1N1B4_4241 0.62 -1.3 4,700,088 - Pf1N1B4_4241 0.62 +1.4 4,700,201 + Pf1N1B4_4241 0.75 -0.8 4,700,210 + Pf1N1B4_4241 0.76 -1.1 4,700,210 + Pf1N1B4_4241 0.76 +0.7 4,700,210 + Pf1N1B4_4241 0.76 -1.5 4,700,211 - Pf1N1B4_4241 0.76 -2.9 4,700,261 + Pf1N1B4_4241 0.82 +0.1 4,700,262 - Pf1N1B4_4241 0.82 +0.3 4,700,262 - Pf1N1B4_4241 0.82 +0.7 4,700,367 - -0.6 4,700,369 + +0.4 4,700,369 + +0.7 4,700,370 - +2.0 4,700,375 + -0.3 4,700,487 + -0.6 4,700,487 + -0.3 4,700,488 - -2.6 4,700,546 + -0.1 4,700,601 + +0.2 4,700,889 - -0.3 4,700,889 - Pf1N1B4_4242 0.15 -0.5 4,700,889 - Pf1N1B4_4242 0.15 -1.9 4,700,943 - Pf1N1B4_4242 0.19 +0.3 4,700,946 + Pf1N1B4_4242 0.19 +1.0 4,700,947 - Pf1N1B4_4242 0.19 -0.1 4,700,947 - Pf1N1B4_4242 0.19 +0.7 4,701,021 - Pf1N1B4_4242 0.24 -1.9 4,701,453 + Pf1N1B4_4242 0.51 -0.7 4,701,454 - +1.7 4,701,486 - +1.3 4,701,486 - Pf1N1B4_4242 0.53 -1.9 4,701,567 - Pf1N1B4_4242 0.59 +0.7 4,701,809 + Pf1N1B4_4242 0.74 -1.3 4,701,812 + Pf1N1B4_4242 0.74 -0.3 4,702,204 + +1.7 4,702,215 + -0.3 4,702,298 + -2.6 4,702,299 - +0.9 4,702,374 + -2.3 4,702,522 + Pf1N1B4_4243 0.13 -1.2 4,702,602 + Pf1N1B4_4243 0.20 +1.1 4,702,655 + -1.3 4,702,655 + Pf1N1B4_4243 0.24 +0.7 4,702,655 + Pf1N1B4_4243 0.24 -0.3 4,702,655 + Pf1N1B4_4243 0.24 +0.8 4,702,656 - Pf1N1B4_4243 0.24 -1.9 4,702,656 - Pf1N1B4_4243 0.24 +1.5 4,702,659 + -0.3 4,702,660 - -1.3 4,702,701 + -0.9 4,702,832 + Pf1N1B4_4243 0.40 -1.2 4,702,873 + Pf1N1B4_4243 0.43 +0.1 4,702,873 + Pf1N1B4_4243 0.43 +0.0 4,702,874 - Pf1N1B4_4243 0.43 -0.6
Or see this region's nucleotide sequence