Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4198 and Pf1N1B4_4199 are separated by 24 nucleotides Pf1N1B4_4199 and Pf1N1B4_4200 are separated by 126 nucleotides
Pf1N1B4_4198 - 4-hydroxybenzoate transporter, at 4,649,037 to 4,650,374
_4198
Pf1N1B4_4199 - Protocatechuate 4,5-dioxygenase beta chain (EC 1.13.11.8), at 4,650,399 to 4,651,661
_4199
Pf1N1B4_4200 - PhaK-like protein, at 4,651,788 to 4,653,035
_4200
Position (kb) 4650
4651
4652 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4649.692 kb on + strand, within Pf1N1B4_4198 at 4649.826 kb on + strand, within Pf1N1B4_4198 at 4649.826 kb on + strand, within Pf1N1B4_4198 at 4649.850 kb on + strand, within Pf1N1B4_4198 at 4649.859 kb on - strand, within Pf1N1B4_4198 at 4650.558 kb on + strand, within Pf1N1B4_4199 at 4650.559 kb on - strand, within Pf1N1B4_4199 at 4651.183 kb on - strand, within Pf1N1B4_4199 at 4651.183 kb on - strand, within Pf1N1B4_4199 at 4651.185 kb on + strand, within Pf1N1B4_4199 at 4651.185 kb on + strand, within Pf1N1B4_4199 at 4651.185 kb on + strand, within Pf1N1B4_4199 at 4651.503 kb on + strand, within Pf1N1B4_4199 at 4651.504 kb on - strand, within Pf1N1B4_4199 at 4651.569 kb on + strand at 4651.783 kb on + strand at 4651.784 kb on - strand at 4651.912 kb on - strand at 4652.215 kb on + strand, within Pf1N1B4_4200 at 4652.215 kb on + strand, within Pf1N1B4_4200 at 4652.216 kb on - strand at 4652.298 kb on + strand, within Pf1N1B4_4200 at 4652.484 kb on + strand, within Pf1N1B4_4200 at 4652.485 kb on - strand, within Pf1N1B4_4200 at 4652.485 kb on - strand, within Pf1N1B4_4200 at 4652.636 kb on + strand, within Pf1N1B4_4200 at 4652.643 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 4,649,692 + Pf1N1B4_4198 0.49 +0.9 4,649,826 + Pf1N1B4_4198 0.59 +0.1 4,649,826 + Pf1N1B4_4198 0.59 +0.9 4,649,850 + Pf1N1B4_4198 0.61 +2.3 4,649,859 - Pf1N1B4_4198 0.61 -1.0 4,650,558 + Pf1N1B4_4199 0.13 -0.3 4,650,559 - Pf1N1B4_4199 0.13 -0.4 4,651,183 - Pf1N1B4_4199 0.62 +0.2 4,651,183 - Pf1N1B4_4199 0.62 -1.6 4,651,185 + Pf1N1B4_4199 0.62 -0.8 4,651,185 + Pf1N1B4_4199 0.62 +0.2 4,651,185 + Pf1N1B4_4199 0.62 -1.2 4,651,503 + Pf1N1B4_4199 0.87 -0.1 4,651,504 - Pf1N1B4_4199 0.87 +0.0 4,651,569 + -2.1 4,651,783 + +0.7 4,651,784 - -0.3 4,651,912 - +0.6 4,652,215 + Pf1N1B4_4200 0.34 +0.7 4,652,215 + Pf1N1B4_4200 0.34 +0.1 4,652,216 - -1.3 4,652,298 + Pf1N1B4_4200 0.41 -1.9 4,652,484 + Pf1N1B4_4200 0.56 -0.3 4,652,485 - Pf1N1B4_4200 0.56 -1.9 4,652,485 - Pf1N1B4_4200 0.56 -0.6 4,652,636 + Pf1N1B4_4200 0.68 +1.4 4,652,643 + +1.1
Or see this region's nucleotide sequence