Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4174 and Pf1N1B4_4175 overlap by 15 nucleotides Pf1N1B4_4175 and Pf1N1B4_4176 overlap by 11 nucleotides Pf1N1B4_4176 and Pf1N1B4_4177 are separated by 33 nucleotides Pf1N1B4_4177 and Pf1N1B4_4178 are separated by 167 nucleotides
Pf1N1B4_4174 - hypothetical protein, at 4,623,579 to 4,623,761
_4174
Pf1N1B4_4175 - Universal stress protein family 5, at 4,623,747 to 4,624,667
_4175
Pf1N1B4_4176 - Alcohol dehydrogenase (EC 1.1.1.1), at 4,624,657 to 4,625,640
_4176
Pf1N1B4_4177 - transcriptional regulator, Crp/Fnr family, at 4,625,674 to 4,626,420
_4177
Pf1N1B4_4178 - Cell division protein FtsH (EC 3.4.24.-), at 4,626,588 to 4,628,375
_4178
Position (kb)
4624
4625
4626 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4623.667 kb on - strand, within Pf1N1B4_4174 at 4623.709 kb on + strand, within Pf1N1B4_4174 at 4623.709 kb on + strand, within Pf1N1B4_4174 at 4623.792 kb on + strand at 4623.868 kb on + strand, within Pf1N1B4_4175 at 4623.868 kb on + strand, within Pf1N1B4_4175 at 4623.868 kb on + strand, within Pf1N1B4_4175 at 4623.868 kb on + strand, within Pf1N1B4_4175 at 4623.869 kb on - strand, within Pf1N1B4_4175 at 4623.869 kb on - strand at 4624.306 kb on + strand at 4624.329 kb on + strand, within Pf1N1B4_4175 at 4624.329 kb on + strand at 4624.329 kb on + strand, within Pf1N1B4_4175 at 4624.329 kb on + strand, within Pf1N1B4_4175 at 4624.330 kb on - strand, within Pf1N1B4_4175 at 4624.525 kb on - strand, within Pf1N1B4_4175 at 4624.525 kb on - strand, within Pf1N1B4_4175 at 4624.543 kb on + strand at 4624.544 kb on - strand, within Pf1N1B4_4175 at 4624.764 kb on + strand, within Pf1N1B4_4176 at 4624.764 kb on + strand, within Pf1N1B4_4176 at 4625.036 kb on + strand at 4625.036 kb on + strand, within Pf1N1B4_4176 at 4625.036 kb on + strand, within Pf1N1B4_4176 at 4625.076 kb on - strand, within Pf1N1B4_4176 at 4625.076 kb on - strand, within Pf1N1B4_4176 at 4625.276 kb on + strand, within Pf1N1B4_4176 at 4625.555 kb on - strand at 4625.643 kb on + strand at 4625.644 kb on - strand at 4625.644 kb on - strand at 4625.644 kb on - strand at 4625.644 kb on - strand at 4625.825 kb on + strand, within Pf1N1B4_4177 at 4625.826 kb on - strand, within Pf1N1B4_4177 at 4626.056 kb on + strand, within Pf1N1B4_4177 at 4626.057 kb on - strand, within Pf1N1B4_4177 at 4626.389 kb on + strand at 4626.425 kb on + strand at 4626.453 kb on + strand at 4626.453 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 4,623,667 - Pf1N1B4_4174 0.48 +0.1 4,623,709 + Pf1N1B4_4174 0.71 +0.7 4,623,709 + Pf1N1B4_4174 0.71 -0.8 4,623,792 + +0.5 4,623,868 + Pf1N1B4_4175 0.13 -0.6 4,623,868 + Pf1N1B4_4175 0.13 -0.7 4,623,868 + Pf1N1B4_4175 0.13 -1.0 4,623,868 + Pf1N1B4_4175 0.13 +0.3 4,623,869 - Pf1N1B4_4175 0.13 +1.3 4,623,869 - -0.3 4,624,306 + +2.5 4,624,329 + Pf1N1B4_4175 0.63 -0.3 4,624,329 + +2.5 4,624,329 + Pf1N1B4_4175 0.63 +1.3 4,624,329 + Pf1N1B4_4175 0.63 -0.6 4,624,330 - Pf1N1B4_4175 0.63 +0.3 4,624,525 - Pf1N1B4_4175 0.84 +0.1 4,624,525 - Pf1N1B4_4175 0.84 +2.7 4,624,543 + -0.3 4,624,544 - Pf1N1B4_4175 0.87 +0.2 4,624,764 + Pf1N1B4_4176 0.11 -2.0 4,624,764 + Pf1N1B4_4176 0.11 -0.3 4,625,036 + +0.1 4,625,036 + Pf1N1B4_4176 0.39 +1.6 4,625,036 + Pf1N1B4_4176 0.39 -0.8 4,625,076 - Pf1N1B4_4176 0.43 +0.3 4,625,076 - Pf1N1B4_4176 0.43 -1.9 4,625,276 + Pf1N1B4_4176 0.63 -1.4 4,625,555 - -0.9 4,625,643 + +1.3 4,625,644 - -0.0 4,625,644 - -1.3 4,625,644 - -0.9 4,625,644 - +0.7 4,625,825 + Pf1N1B4_4177 0.20 -0.3 4,625,826 - Pf1N1B4_4177 0.20 -0.9 4,626,056 + Pf1N1B4_4177 0.51 -0.6 4,626,057 - Pf1N1B4_4177 0.51 +0.1 4,626,389 + -0.3 4,626,425 + -0.3 4,626,453 + +0.1 4,626,453 + -0.3
Or see this region's nucleotide sequence