Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3810 and Pf1N1B4_3811 are separated by 464 nucleotides Pf1N1B4_3811 and Pf1N1B4_3812 are separated by 79 nucleotides Pf1N1B4_3812 and Pf1N1B4_3813 are separated by 174 nucleotides Pf1N1B4_3813 and Pf1N1B4_3814 are separated by 92 nucleotides
Pf1N1B4_3810 - Transcriptional regulator, LysR family, at 4,221,163 to 4,222,089
_3810
Pf1N1B4_3811 - Hydantoin racemase (EC 5.1.99.-), at 4,222,554 to 4,222,910
_3811
Pf1N1B4_3812 - Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8) / Adenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.10), at 4,222,990 to 4,223,730
_3812
Pf1N1B4_3813 - Homoserine kinase (EC 2.7.1.39), at 4,223,905 to 4,224,522
_3813
Pf1N1B4_3814 - Para-aminobenzoate synthase, aminase component (EC 2.6.1.85), at 4,224,615 to 4,225,952
_3814
Position (kb)
4222
4223
4224 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4222.123 kb on + strand at 4222.124 kb on - strand at 4222.148 kb on + strand at 4222.149 kb on - strand at 4222.149 kb on - strand at 4222.149 kb on - strand at 4222.149 kb on - strand at 4222.156 kb on - strand at 4222.156 kb on - strand at 4222.156 kb on - strand at 4222.172 kb on - strand at 4222.207 kb on - strand at 4222.207 kb on - strand at 4222.247 kb on - strand at 4222.247 kb on - strand at 4222.247 kb on - strand at 4222.303 kb on + strand at 4222.311 kb on - strand at 4222.311 kb on - strand at 4222.325 kb on - strand at 4222.354 kb on - strand at 4222.354 kb on - strand at 4222.357 kb on + strand at 4222.357 kb on + strand at 4222.357 kb on + strand at 4222.357 kb on + strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.358 kb on - strand at 4222.370 kb on + strand at 4222.403 kb on - strand at 4222.440 kb on - strand at 4222.447 kb on - strand at 4222.447 kb on - strand at 4222.556 kb on - strand at 4222.556 kb on - strand at 4222.556 kb on - strand at 4222.556 kb on - strand at 4222.556 kb on - strand at 4222.590 kb on - strand at 4222.590 kb on - strand, within Pf1N1B4_3811 at 4222.651 kb on + strand, within Pf1N1B4_3811 at 4222.652 kb on - strand, within Pf1N1B4_3811 at 4222.652 kb on - strand, within Pf1N1B4_3811 at 4222.652 kb on - strand, within Pf1N1B4_3811 at 4222.965 kb on + strand at 4222.967 kb on + strand at 4222.967 kb on + strand at 4222.967 kb on + strand at 4222.967 kb on + strand at 4222.967 kb on + strand at 4222.967 kb on + strand at 4222.967 kb on + strand at 4222.967 kb on + strand at 4222.967 kb on + strand at 4222.967 kb on + strand at 4222.968 kb on - strand at 4222.978 kb on + strand at 4222.978 kb on + strand at 4222.978 kb on + strand at 4222.978 kb on + strand at 4222.979 kb on - strand at 4222.979 kb on - strand at 4222.991 kb on + strand at 4223.156 kb on + strand, within Pf1N1B4_3812 at 4223.156 kb on + strand, within Pf1N1B4_3812 at 4223.156 kb on + strand, within Pf1N1B4_3812 at 4223.157 kb on - strand, within Pf1N1B4_3812 at 4223.157 kb on - strand, within Pf1N1B4_3812 at 4223.157 kb on - strand, within Pf1N1B4_3812 at 4223.180 kb on + strand, within Pf1N1B4_3812 at 4223.180 kb on + strand, within Pf1N1B4_3812 at 4223.181 kb on - strand, within Pf1N1B4_3812 at 4223.229 kb on - strand, within Pf1N1B4_3812 at 4223.229 kb on - strand, within Pf1N1B4_3812 at 4223.405 kb on + strand, within Pf1N1B4_3812 at 4223.405 kb on + strand, within Pf1N1B4_3812 at 4223.405 kb on + strand, within Pf1N1B4_3812 at 4223.405 kb on + strand, within Pf1N1B4_3812 at 4223.405 kb on + strand, within Pf1N1B4_3812 at 4223.405 kb on + strand, within Pf1N1B4_3812 at 4223.406 kb on - strand, within Pf1N1B4_3812 at 4223.406 kb on - strand, within Pf1N1B4_3812 at 4223.406 kb on - strand, within Pf1N1B4_3812 at 4223.453 kb on + strand, within Pf1N1B4_3812 at 4223.454 kb on - strand, within Pf1N1B4_3812 at 4223.454 kb on - strand, within Pf1N1B4_3812 at 4223.454 kb on - strand at 4223.486 kb on + strand, within Pf1N1B4_3812 at 4223.486 kb on + strand, within Pf1N1B4_3812 at 4223.486 kb on + strand, within Pf1N1B4_3812 at 4223.486 kb on + strand, within Pf1N1B4_3812 at 4223.486 kb on + strand, within Pf1N1B4_3812 at 4223.486 kb on + strand, within Pf1N1B4_3812 at 4223.486 kb on + strand, within Pf1N1B4_3812 at 4223.487 kb on - strand, within Pf1N1B4_3812 at 4223.487 kb on - strand, within Pf1N1B4_3812 at 4223.487 kb on - strand, within Pf1N1B4_3812 at 4223.487 kb on - strand, within Pf1N1B4_3812 at 4223.487 kb on - strand, within Pf1N1B4_3812 at 4223.487 kb on - strand, within Pf1N1B4_3812 at 4223.487 kb on - strand, within Pf1N1B4_3812 at 4223.487 kb on - strand, within Pf1N1B4_3812 at 4223.487 kb on - strand, within Pf1N1B4_3812 at 4223.487 kb on - strand, within Pf1N1B4_3812 at 4223.487 kb on - strand, within Pf1N1B4_3812 at 4223.516 kb on + strand, within Pf1N1B4_3812 at 4223.516 kb on + strand, within Pf1N1B4_3812 at 4223.517 kb on - strand, within Pf1N1B4_3812 at 4223.517 kb on - strand, within Pf1N1B4_3812 at 4223.623 kb on + strand, within Pf1N1B4_3812 at 4223.690 kb on + strand at 4223.690 kb on + strand at 4223.690 kb on + strand at 4223.690 kb on + strand at 4223.690 kb on + strand at 4223.690 kb on + strand at 4223.690 kb on + strand at 4223.690 kb on + strand at 4223.690 kb on + strand at 4223.690 kb on + strand at 4223.691 kb on - strand at 4223.691 kb on - strand at 4223.691 kb on - strand at 4223.691 kb on - strand at 4223.691 kb on - strand at 4223.691 kb on - strand at 4223.744 kb on + strand at 4223.744 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.769 kb on + strand at 4223.770 kb on - strand at 4223.770 kb on - strand at 4223.770 kb on - strand at 4223.770 kb on - strand at 4223.770 kb on - strand at 4223.770 kb on - strand at 4223.770 kb on - strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.771 kb on + strand at 4223.772 kb on - strand at 4223.772 kb on - strand at 4223.772 kb on - strand at 4223.772 kb on - strand at 4223.772 kb on - strand at 4223.772 kb on - strand at 4223.772 kb on - strand at 4223.772 kb on - strand at 4223.772 kb on - strand at 4223.772 kb on - strand at 4223.772 kb on - strand at 4223.772 kb on - strand at 4224.334 kb on + strand, within Pf1N1B4_3813 at 4224.334 kb on + strand, within Pf1N1B4_3813 at 4224.334 kb on + strand, within Pf1N1B4_3813 at 4224.335 kb on - strand, within Pf1N1B4_3813 at 4224.337 kb on + strand, within Pf1N1B4_3813 at 4224.364 kb on + strand, within Pf1N1B4_3813 at 4224.364 kb on + strand, within Pf1N1B4_3813 at 4224.364 kb on + strand, within Pf1N1B4_3813 at 4224.365 kb on - strand, within Pf1N1B4_3813 at 4224.365 kb on - strand, within Pf1N1B4_3813 at 4224.372 kb on + strand, within Pf1N1B4_3813 at 4224.520 kb on + strand at 4224.583 kb on - strand at 4224.656 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 4,222,123 + +0.1 4,222,124 - +0.8 4,222,148 + -1.8 4,222,149 - +0.1 4,222,149 - -0.2 4,222,149 - -0.4 4,222,149 - -1.1 4,222,156 - +0.8 4,222,156 - +0.8 4,222,156 - +0.4 4,222,172 - -2.2 4,222,207 - +0.5 4,222,207 - +0.0 4,222,247 - +0.8 4,222,247 - -0.8 4,222,247 - -0.2 4,222,303 + +1.4 4,222,311 - -2.5 4,222,311 - +1.6 4,222,325 - -0.2 4,222,354 - -0.8 4,222,354 - -0.9 4,222,357 + +2.8 4,222,357 + +1.4 4,222,357 + -0.2 4,222,357 + +0.1 4,222,358 - +0.4 4,222,358 - -0.8 4,222,358 - -0.7 4,222,358 - +0.6 4,222,358 - -0.8 4,222,358 - -0.4 4,222,358 - -0.6 4,222,358 - -2.2 4,222,358 - -1.4 4,222,358 - +2.0 4,222,358 - +0.5 4,222,358 - +0.4 4,222,358 - +0.5 4,222,370 + +1.0 4,222,403 - -0.2 4,222,440 - +0.4 4,222,447 - -1.2 4,222,447 - -2.2 4,222,556 - -0.6 4,222,556 - +0.1 4,222,556 - +0.5 4,222,556 - -0.0 4,222,556 - +0.4 4,222,590 - +1.4 4,222,590 - Pf1N1B4_3811 0.10 -0.0 4,222,651 + Pf1N1B4_3811 0.27 -1.0 4,222,652 - Pf1N1B4_3811 0.27 +0.4 4,222,652 - Pf1N1B4_3811 0.27 +2.1 4,222,652 - Pf1N1B4_3811 0.27 +0.2 4,222,965 + +1.8 4,222,967 + +0.2 4,222,967 + +2.1 4,222,967 + +0.8 4,222,967 + -0.3 4,222,967 + -0.8 4,222,967 + -0.2 4,222,967 + -1.4 4,222,967 + -0.6 4,222,967 + -0.6 4,222,967 + +2.4 4,222,968 - +1.3 4,222,978 + -1.2 4,222,978 + -0.9 4,222,978 + -0.2 4,222,978 + +0.8 4,222,979 - -0.2 4,222,979 - -1.5 4,222,991 + +0.0 4,223,156 + Pf1N1B4_3812 0.22 -3.0 4,223,156 + Pf1N1B4_3812 0.22 -0.2 4,223,156 + Pf1N1B4_3812 0.22 -3.0 4,223,157 - Pf1N1B4_3812 0.23 -2.2 4,223,157 - Pf1N1B4_3812 0.23 -1.8 4,223,157 - Pf1N1B4_3812 0.23 -0.2 4,223,180 + Pf1N1B4_3812 0.26 -1.2 4,223,180 + Pf1N1B4_3812 0.26 -1.2 4,223,181 - Pf1N1B4_3812 0.26 -0.9 4,223,229 - Pf1N1B4_3812 0.32 -0.2 4,223,229 - Pf1N1B4_3812 0.32 -2.5 4,223,405 + Pf1N1B4_3812 0.56 +1.4 4,223,405 + Pf1N1B4_3812 0.56 -2.0 4,223,405 + Pf1N1B4_3812 0.56 -1.8 4,223,405 + Pf1N1B4_3812 0.56 -0.9 4,223,405 + Pf1N1B4_3812 0.56 -2.8 4,223,405 + Pf1N1B4_3812 0.56 -2.0 4,223,406 - Pf1N1B4_3812 0.56 -0.2 4,223,406 - Pf1N1B4_3812 0.56 -2.5 4,223,406 - Pf1N1B4_3812 0.56 -1.4 4,223,453 + Pf1N1B4_3812 0.62 -2.4 4,223,454 - Pf1N1B4_3812 0.63 -0.8 4,223,454 - Pf1N1B4_3812 0.63 -0.2 4,223,454 - -1.2 4,223,486 + Pf1N1B4_3812 0.67 -2.8 4,223,486 + Pf1N1B4_3812 0.67 +1.4 4,223,486 + Pf1N1B4_3812 0.67 +0.3 4,223,486 + Pf1N1B4_3812 0.67 -1.9 4,223,486 + Pf1N1B4_3812 0.67 -2.8 4,223,486 + Pf1N1B4_3812 0.67 -0.8 4,223,486 + Pf1N1B4_3812 0.67 -2.2 4,223,487 - Pf1N1B4_3812 0.67 -1.5 4,223,487 - Pf1N1B4_3812 0.67 -2.2 4,223,487 - Pf1N1B4_3812 0.67 +0.8 4,223,487 - Pf1N1B4_3812 0.67 +0.8 4,223,487 - Pf1N1B4_3812 0.67 -1.8 4,223,487 - Pf1N1B4_3812 0.67 -0.2 4,223,487 - Pf1N1B4_3812 0.67 +0.2 4,223,487 - Pf1N1B4_3812 0.67 -2.9 4,223,487 - Pf1N1B4_3812 0.67 -1.8 4,223,487 - Pf1N1B4_3812 0.67 -2.2 4,223,487 - Pf1N1B4_3812 0.67 -1.2 4,223,516 + Pf1N1B4_3812 0.71 -1.2 4,223,516 + Pf1N1B4_3812 0.71 -1.0 4,223,517 - Pf1N1B4_3812 0.71 -0.9 4,223,517 - Pf1N1B4_3812 0.71 -1.8 4,223,623 + Pf1N1B4_3812 0.85 -1.2 4,223,690 + -4.1 4,223,690 + -2.8 4,223,690 + -0.2 4,223,690 + -1.5 4,223,690 + -2.2 4,223,690 + -0.6 4,223,690 + -2.6 4,223,690 + -1.2 4,223,690 + -2.0 4,223,690 + -3.2 4,223,691 - -1.6 4,223,691 - +0.8 4,223,691 - -1.2 4,223,691 - -2.5 4,223,691 - -0.5 4,223,691 - -0.2 4,223,744 + -1.8 4,223,744 + -2.9 4,223,769 + -3.0 4,223,769 + +1.0 4,223,769 + -1.2 4,223,769 + -0.8 4,223,769 + +0.1 4,223,769 + -1.8 4,223,769 + -0.5 4,223,769 + -3.0 4,223,769 + -1.4 4,223,769 + -2.4 4,223,769 + -3.0 4,223,769 + -3.7 4,223,769 + -2.7 4,223,769 + -1.4 4,223,769 + -2.5 4,223,769 + -1.9 4,223,769 + -1.5 4,223,770 - -0.8 4,223,770 - +1.8 4,223,770 - +1.5 4,223,770 - +1.1 4,223,770 - +0.8 4,223,770 - -1.5 4,223,770 - +0.2 4,223,771 + +0.5 4,223,771 + -0.9 4,223,771 + -1.8 4,223,771 + -2.6 4,223,771 + -1.4 4,223,771 + -1.6 4,223,771 + -1.2 4,223,771 + -1.5 4,223,771 + +0.4 4,223,771 + -3.0 4,223,771 + -1.5 4,223,771 + -1.2 4,223,771 + -2.3 4,223,771 + -2.1 4,223,771 + -1.9 4,223,771 + -1.3 4,223,771 + -1.9 4,223,771 + -1.6 4,223,771 + -1.7 4,223,771 + -2.8 4,223,771 + -1.5 4,223,771 + -2.3 4,223,771 + -0.2 4,223,771 + -0.2 4,223,771 + -3.3 4,223,771 + -1.6 4,223,771 + -1.6 4,223,771 + -1.8 4,223,771 + -0.6 4,223,771 + -2.5 4,223,771 + -3.0 4,223,771 + -0.4 4,223,771 + -2.2 4,223,771 + -0.2 4,223,771 + -1.6 4,223,771 + -2.0 4,223,771 + -3.2 4,223,772 - -0.6 4,223,772 - -0.6 4,223,772 - -0.8 4,223,772 - -1.2 4,223,772 - -0.5 4,223,772 - -0.2 4,223,772 - +1.0 4,223,772 - -0.2 4,223,772 - +0.4 4,223,772 - +0.8 4,223,772 - -0.8 4,223,772 - +0.2 4,224,334 + Pf1N1B4_3813 0.69 +1.9 4,224,334 + Pf1N1B4_3813 0.69 +0.0 4,224,334 + Pf1N1B4_3813 0.69 -0.2 4,224,335 - Pf1N1B4_3813 0.70 +1.4 4,224,337 + Pf1N1B4_3813 0.70 -0.3 4,224,364 + Pf1N1B4_3813 0.74 -0.7 4,224,364 + Pf1N1B4_3813 0.74 +1.4 4,224,364 + Pf1N1B4_3813 0.74 -0.8 4,224,365 - Pf1N1B4_3813 0.74 +1.4 4,224,365 - Pf1N1B4_3813 0.74 +0.5 4,224,372 + Pf1N1B4_3813 0.76 +0.8 4,224,520 + -1.2 4,224,583 - -0.1 4,224,656 + -0.5
Or see this region's nucleotide sequence