Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3490 and Pf1N1B4_3491 are separated by 420 nucleotides Pf1N1B4_3491 and Pf1N1B4_3492 are separated by 131 nucleotides Pf1N1B4_3492 and Pf1N1B4_3493 are separated by 115 nucleotides
Pf1N1B4_3490 - probable membrane protein YPO2362, at 3,897,816 to 3,898,418
_3490
Pf1N1B4_3491 - tRNA(Cytosine32)-2-thiocytidine synthetase, at 3,898,839 to 3,899,663
_3491
Pf1N1B4_3492 - DNA-3-methyladenine glycosylase (EC 3.2.2.20), at 3,899,795 to 3,900,469
_3492
Pf1N1B4_3493 - FIG00956341: hypothetical protein, at 3,900,585 to 3,901,010
_3493
Position (kb)
3898
3899
3900 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand at 3897.971 kb on - strand, within Pf1N1B4_3490 at 3897.971 kb on - strand, within Pf1N1B4_3490 at 3897.971 kb on - strand, within Pf1N1B4_3490 at 3897.971 kb on - strand, within Pf1N1B4_3490 at 3897.979 kb on + strand, within Pf1N1B4_3490 at 3897.979 kb on + strand, within Pf1N1B4_3490 at 3897.979 kb on + strand at 3897.980 kb on - strand, within Pf1N1B4_3490 at 3897.989 kb on - strand, within Pf1N1B4_3490 at 3897.989 kb on - strand, within Pf1N1B4_3490 at 3898.078 kb on + strand, within Pf1N1B4_3490 at 3898.123 kb on + strand, within Pf1N1B4_3490 at 3898.124 kb on - strand, within Pf1N1B4_3490 at 3898.124 kb on - strand, within Pf1N1B4_3490 at 3898.124 kb on - strand, within Pf1N1B4_3490 at 3898.124 kb on - strand, within Pf1N1B4_3490 at 3898.124 kb on - strand, within Pf1N1B4_3490 at 3898.124 kb on - strand at 3898.187 kb on - strand, within Pf1N1B4_3490 at 3898.327 kb on + strand, within Pf1N1B4_3490 at 3898.327 kb on + strand, within Pf1N1B4_3490 at 3898.328 kb on - strand, within Pf1N1B4_3490 at 3898.328 kb on - strand, within Pf1N1B4_3490 at 3898.328 kb on - strand, within Pf1N1B4_3490 at 3898.328 kb on - strand, within Pf1N1B4_3490 at 3898.328 kb on - strand, within Pf1N1B4_3490 at 3898.328 kb on - strand, within Pf1N1B4_3490 at 3898.328 kb on - strand at 3898.404 kb on + strand at 3898.404 kb on + strand at 3898.404 kb on + strand at 3898.404 kb on + strand at 3898.404 kb on + strand at 3898.404 kb on + strand at 3898.404 kb on + strand at 3898.405 kb on - strand at 3898.405 kb on - strand at 3898.405 kb on - strand at 3898.485 kb on + strand at 3898.485 kb on + strand at 3898.485 kb on + strand at 3898.485 kb on + strand at 3898.485 kb on + strand at 3898.485 kb on + strand at 3898.486 kb on - strand at 3898.486 kb on - strand at 3898.486 kb on - strand at 3898.486 kb on - strand at 3898.486 kb on - strand at 3898.486 kb on - strand at 3898.489 kb on + strand at 3898.490 kb on - strand at 3898.490 kb on - strand at 3898.490 kb on - strand at 3898.490 kb on - strand at 3898.501 kb on + strand at 3898.501 kb on + strand at 3898.505 kb on + strand at 3898.506 kb on - strand at 3898.515 kb on + strand at 3898.527 kb on + strand at 3898.527 kb on + strand at 3898.528 kb on - strand at 3898.528 kb on - strand at 3898.528 kb on - strand at 3898.528 kb on - strand at 3898.528 kb on - strand at 3898.547 kb on + strand at 3898.547 kb on + strand at 3898.547 kb on + strand at 3898.548 kb on - strand at 3898.548 kb on - strand at 3898.548 kb on - strand at 3898.548 kb on - strand at 3898.561 kb on + strand at 3898.578 kb on + strand at 3898.579 kb on - strand at 3898.585 kb on + strand at 3898.763 kb on + strand at 3898.764 kb on - strand at 3898.850 kb on + strand at 3898.850 kb on + strand at 3898.974 kb on + strand at 3898.974 kb on + strand, within Pf1N1B4_3491 at 3898.974 kb on + strand, within Pf1N1B4_3491 at 3898.975 kb on - strand, within Pf1N1B4_3491 at 3898.975 kb on - strand, within Pf1N1B4_3491 at 3898.975 kb on - strand at 3898.975 kb on - strand, within Pf1N1B4_3491 at 3899.091 kb on + strand, within Pf1N1B4_3491 at 3899.091 kb on + strand, within Pf1N1B4_3491 at 3899.139 kb on + strand, within Pf1N1B4_3491 at 3899.139 kb on + strand, within Pf1N1B4_3491 at 3899.140 kb on - strand, within Pf1N1B4_3491 at 3899.159 kb on + strand, within Pf1N1B4_3491 at 3899.217 kb on + strand, within Pf1N1B4_3491 at 3899.654 kb on - strand at 3899.654 kb on - strand at 3899.654 kb on - strand at 3899.769 kb on + strand at 3899.770 kb on - strand at 3899.777 kb on + strand at 3899.777 kb on + strand at 3899.777 kb on + strand at 3899.777 kb on + strand at 3899.777 kb on + strand at 3899.777 kb on + strand at 3899.777 kb on + strand at 3899.777 kb on + strand at 3899.778 kb on - strand at 3899.778 kb on - strand at 3899.778 kb on - strand at 3899.778 kb on - strand at 3899.778 kb on - strand at 3899.778 kb on - strand at 3899.778 kb on - strand at 3899.778 kb on - strand at 3899.778 kb on - strand at 3899.805 kb on - strand at 3899.805 kb on - strand at 3899.805 kb on - strand at 3899.823 kb on - strand at 3900.215 kb on + strand at 3900.215 kb on + strand, within Pf1N1B4_3492 at 3900.215 kb on + strand, within Pf1N1B4_3492 at 3900.216 kb on - strand, within Pf1N1B4_3492 at 3900.216 kb on - strand, within Pf1N1B4_3492 at 3900.216 kb on - strand, within Pf1N1B4_3492 at 3900.302 kb on + strand, within Pf1N1B4_3492 at 3900.302 kb on + strand, within Pf1N1B4_3492 at 3900.303 kb on - strand, within Pf1N1B4_3492 at 3900.355 kb on + strand, within Pf1N1B4_3492 at 3900.356 kb on - strand, within Pf1N1B4_3492 at 3900.512 kb on - strand at 3900.512 kb on - strand at 3900.581 kb on + strand at 3900.649 kb on + strand, within Pf1N1B4_3493 at 3900.649 kb on + strand, within Pf1N1B4_3493 at 3900.650 kb on - strand, within Pf1N1B4_3493 at 3900.650 kb on - strand, within Pf1N1B4_3493 at 3900.650 kb on - strand, within Pf1N1B4_3493 at 3900.650 kb on - strand, within Pf1N1B4_3493 at 3900.650 kb on - strand, within Pf1N1B4_3493 at 3900.650 kb on - strand, within Pf1N1B4_3493 at 3900.650 kb on - strand, within Pf1N1B4_3493 at 3900.650 kb on - strand at 3900.650 kb on - strand, within Pf1N1B4_3493 at 3900.650 kb on - strand, within Pf1N1B4_3493 at 3900.650 kb on - strand, within Pf1N1B4_3493
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 3,897,970 + Pf1N1B4_3490 0.26 -1.1 3,897,970 + Pf1N1B4_3490 0.26 -0.9 3,897,970 + Pf1N1B4_3490 0.26 -2.4 3,897,970 + Pf1N1B4_3490 0.26 -1.1 3,897,970 + Pf1N1B4_3490 0.26 +1.2 3,897,970 + Pf1N1B4_3490 0.26 -1.1 3,897,970 + Pf1N1B4_3490 0.26 +1.7 3,897,970 + +2.2 3,897,971 - Pf1N1B4_3490 0.26 -1.0 3,897,971 - Pf1N1B4_3490 0.26 -1.1 3,897,971 - Pf1N1B4_3490 0.26 +0.7 3,897,971 - Pf1N1B4_3490 0.26 -0.1 3,897,979 + Pf1N1B4_3490 0.27 +2.2 3,897,979 + Pf1N1B4_3490 0.27 -0.5 3,897,979 + +2.9 3,897,980 - Pf1N1B4_3490 0.27 +0.2 3,897,989 - Pf1N1B4_3490 0.29 -1.9 3,897,989 - Pf1N1B4_3490 0.29 +1.5 3,898,078 + Pf1N1B4_3490 0.43 -0.9 3,898,123 + Pf1N1B4_3490 0.51 +0.5 3,898,124 - Pf1N1B4_3490 0.51 +2.5 3,898,124 - Pf1N1B4_3490 0.51 +1.5 3,898,124 - Pf1N1B4_3490 0.51 +0.9 3,898,124 - Pf1N1B4_3490 0.51 -1.4 3,898,124 - Pf1N1B4_3490 0.51 +2.0 3,898,124 - +2.2 3,898,187 - Pf1N1B4_3490 0.62 -0.4 3,898,327 + Pf1N1B4_3490 0.85 -0.1 3,898,327 + Pf1N1B4_3490 0.85 +2.0 3,898,328 - Pf1N1B4_3490 0.85 -1.4 3,898,328 - Pf1N1B4_3490 0.85 +1.5 3,898,328 - Pf1N1B4_3490 0.85 +0.7 3,898,328 - Pf1N1B4_3490 0.85 +2.1 3,898,328 - Pf1N1B4_3490 0.85 +2.7 3,898,328 - Pf1N1B4_3490 0.85 +0.1 3,898,328 - -1.1 3,898,404 + -1.1 3,898,404 + +1.2 3,898,404 + +0.1 3,898,404 + -1.3 3,898,404 + +0.9 3,898,404 + +0.6 3,898,404 + +0.3 3,898,405 - -0.4 3,898,405 - -2.9 3,898,405 - -1.3 3,898,485 + +1.6 3,898,485 + -0.1 3,898,485 + +2.2 3,898,485 + -0.4 3,898,485 + +0.3 3,898,485 + +0.9 3,898,486 - +0.6 3,898,486 - -0.1 3,898,486 - +1.9 3,898,486 - -0.5 3,898,486 - +1.7 3,898,486 - +0.9 3,898,489 + -0.1 3,898,490 - +0.9 3,898,490 - -1.1 3,898,490 - +0.3 3,898,490 - -1.9 3,898,501 + -1.1 3,898,501 + +2.7 3,898,505 + +2.3 3,898,506 - -0.9 3,898,515 + +0.1 3,898,527 + -0.1 3,898,527 + +1.2 3,898,528 - -1.0 3,898,528 - -1.7 3,898,528 - +1.5 3,898,528 - -1.1 3,898,528 - +1.5 3,898,547 + -2.1 3,898,547 + +0.2 3,898,547 + +2.7 3,898,548 - +0.2 3,898,548 - -1.5 3,898,548 - -1.7 3,898,548 - -0.4 3,898,561 + +1.5 3,898,578 + -2.3 3,898,579 - -2.1 3,898,585 + -0.6 3,898,763 + -1.7 3,898,764 - +1.2 3,898,850 + -0.7 3,898,850 + -0.7 3,898,974 + +1.5 3,898,974 + Pf1N1B4_3491 0.16 -1.1 3,898,974 + Pf1N1B4_3491 0.16 -0.1 3,898,975 - Pf1N1B4_3491 0.16 -2.9 3,898,975 - Pf1N1B4_3491 0.16 -1.5 3,898,975 - -0.1 3,898,975 - Pf1N1B4_3491 0.16 +0.2 3,899,091 + Pf1N1B4_3491 0.31 -1.7 3,899,091 + Pf1N1B4_3491 0.31 -2.7 3,899,139 + Pf1N1B4_3491 0.36 -2.8 3,899,139 + Pf1N1B4_3491 0.36 -1.1 3,899,140 - Pf1N1B4_3491 0.36 +0.3 3,899,159 + Pf1N1B4_3491 0.39 -2.4 3,899,217 + Pf1N1B4_3491 0.46 +0.1 3,899,654 - -1.4 3,899,654 - -0.4 3,899,654 - -1.1 3,899,769 + -1.7 3,899,770 - +0.3 3,899,777 + +1.1 3,899,777 + +0.2 3,899,777 + -0.1 3,899,777 + -0.1 3,899,777 + -0.3 3,899,777 + +0.9 3,899,777 + -0.1 3,899,777 + +1.2 3,899,778 - +1.8 3,899,778 - +0.9 3,899,778 - -2.4 3,899,778 - -1.1 3,899,778 - -1.1 3,899,778 - +0.5 3,899,778 - -0.7 3,899,778 - -0.1 3,899,778 - -2.3 3,899,805 - -1.9 3,899,805 - +0.4 3,899,805 - -0.9 3,899,823 - +0.9 3,900,215 + -0.1 3,900,215 + Pf1N1B4_3492 0.62 +1.9 3,900,215 + Pf1N1B4_3492 0.62 +0.3 3,900,216 - Pf1N1B4_3492 0.62 -0.1 3,900,216 - Pf1N1B4_3492 0.62 +0.6 3,900,216 - Pf1N1B4_3492 0.62 -0.1 3,900,302 + Pf1N1B4_3492 0.75 -0.2 3,900,302 + Pf1N1B4_3492 0.75 +2.7 3,900,303 - Pf1N1B4_3492 0.75 +0.9 3,900,355 + Pf1N1B4_3492 0.83 -2.1 3,900,356 - Pf1N1B4_3492 0.83 +0.2 3,900,512 - -1.6 3,900,512 - -0.9 3,900,581 + -1.1 3,900,649 + Pf1N1B4_3493 0.15 +0.3 3,900,649 + Pf1N1B4_3493 0.15 +1.2 3,900,650 - Pf1N1B4_3493 0.15 -1.1 3,900,650 - Pf1N1B4_3493 0.15 +0.1 3,900,650 - Pf1N1B4_3493 0.15 -0.2 3,900,650 - Pf1N1B4_3493 0.15 -0.4 3,900,650 - Pf1N1B4_3493 0.15 -1.1 3,900,650 - Pf1N1B4_3493 0.15 -2.1 3,900,650 - Pf1N1B4_3493 0.15 +1.2 3,900,650 - +1.5 3,900,650 - Pf1N1B4_3493 0.15 -0.1 3,900,650 - Pf1N1B4_3493 0.15 +1.2 3,900,650 - Pf1N1B4_3493 0.15 -1.7
Or see this region's nucleotide sequence