Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3210 and Pf1N1B4_3211 are separated by 25 nucleotides Pf1N1B4_3211 and Pf1N1B4_3212 are separated by 116 nucleotides Pf1N1B4_3212 and Pf1N1B4_3213 overlap by 7 nucleotides Pf1N1B4_3213 and Pf1N1B4_3214 are separated by 369 nucleotides
Pf1N1B4_3210 - Uncharacterized protein conserved in bacteria, NMA0228-like, at 3,593,179 to 3,594,033
_3210
Pf1N1B4_3211 - Putative signal peptide protein, at 3,594,059 to 3,594,364
_3211
Pf1N1B4_3212 - hypothetical protein, at 3,594,481 to 3,595,173
_3212
Pf1N1B4_3213 - hypothetical protein, at 3,595,167 to 3,595,376
_3213
Pf1N1B4_3214 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 3,595,746 to 3,596,447
_3214
Position (kb)
3594
3595
3596 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3593.489 kb on + strand, within Pf1N1B4_3210 at 3593.490 kb on - strand, within Pf1N1B4_3210 at 3593.490 kb on - strand, within Pf1N1B4_3210 at 3593.490 kb on - strand, within Pf1N1B4_3210 at 3593.490 kb on - strand, within Pf1N1B4_3210 at 3593.588 kb on + strand, within Pf1N1B4_3210 at 3593.588 kb on + strand, within Pf1N1B4_3210 at 3593.589 kb on - strand, within Pf1N1B4_3210 at 3593.589 kb on - strand, within Pf1N1B4_3210 at 3593.589 kb on - strand, within Pf1N1B4_3210 at 3593.646 kb on + strand, within Pf1N1B4_3210 at 3593.647 kb on - strand, within Pf1N1B4_3210 at 3593.682 kb on - strand, within Pf1N1B4_3210 at 3593.826 kb on + strand, within Pf1N1B4_3210 at 3593.826 kb on + strand, within Pf1N1B4_3210 at 3593.826 kb on + strand, within Pf1N1B4_3210 at 3593.826 kb on + strand, within Pf1N1B4_3210 at 3593.827 kb on - strand, within Pf1N1B4_3210 at 3593.858 kb on + strand, within Pf1N1B4_3210 at 3593.858 kb on + strand, within Pf1N1B4_3210 at 3593.858 kb on + strand, within Pf1N1B4_3210 at 3593.912 kb on + strand, within Pf1N1B4_3210 at 3593.912 kb on + strand at 3593.912 kb on + strand at 3593.912 kb on + strand, within Pf1N1B4_3210 at 3593.913 kb on - strand, within Pf1N1B4_3210 at 3593.913 kb on - strand, within Pf1N1B4_3210 at 3593.913 kb on - strand, within Pf1N1B4_3210 at 3594.075 kb on + strand at 3594.075 kb on + strand at 3594.075 kb on + strand at 3594.076 kb on - strand at 3594.076 kb on - strand at 3594.076 kb on - strand at 3594.076 kb on - strand at 3594.076 kb on - strand at 3594.076 kb on - strand at 3594.196 kb on + strand, within Pf1N1B4_3211 at 3594.196 kb on + strand, within Pf1N1B4_3211 at 3594.196 kb on + strand, within Pf1N1B4_3211 at 3594.196 kb on + strand, within Pf1N1B4_3211 at 3594.197 kb on - strand, within Pf1N1B4_3211 at 3594.197 kb on - strand, within Pf1N1B4_3211 at 3594.197 kb on - strand, within Pf1N1B4_3211 at 3594.197 kb on - strand, within Pf1N1B4_3211 at 3594.197 kb on - strand at 3594.197 kb on - strand, within Pf1N1B4_3211 at 3594.199 kb on + strand, within Pf1N1B4_3211 at 3594.413 kb on + strand at 3594.413 kb on + strand at 3594.414 kb on - strand at 3594.498 kb on + strand at 3594.593 kb on + strand at 3594.594 kb on - strand, within Pf1N1B4_3212 at 3594.594 kb on - strand, within Pf1N1B4_3212 at 3594.620 kb on + strand, within Pf1N1B4_3212 at 3594.696 kb on - strand, within Pf1N1B4_3212 at 3594.785 kb on + strand, within Pf1N1B4_3212 at 3594.786 kb on - strand, within Pf1N1B4_3212 at 3594.870 kb on - strand, within Pf1N1B4_3212 at 3594.870 kb on - strand, within Pf1N1B4_3212 at 3595.148 kb on - strand at 3595.227 kb on - strand, within Pf1N1B4_3213 at 3595.433 kb on - strand at 3595.437 kb on - strand at 3595.504 kb on - strand at 3595.596 kb on - strand at 3595.650 kb on + strand at 3595.650 kb on + strand at 3595.650 kb on + strand at 3595.650 kb on + strand at 3595.662 kb on - strand at 3595.700 kb on + strand at 3595.759 kb on + strand at 3595.837 kb on + strand, within Pf1N1B4_3214 at 3595.837 kb on + strand at 3595.837 kb on + strand, within Pf1N1B4_3214 at 3595.838 kb on - strand, within Pf1N1B4_3214 at 3595.838 kb on - strand, within Pf1N1B4_3214 at 3596.122 kb on + strand, within Pf1N1B4_3214
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 3,593,489 + Pf1N1B4_3210 0.36 -1.0 3,593,490 - Pf1N1B4_3210 0.36 +0.6 3,593,490 - Pf1N1B4_3210 0.36 +1.0 3,593,490 - Pf1N1B4_3210 0.36 -0.2 3,593,490 - Pf1N1B4_3210 0.36 -0.3 3,593,588 + Pf1N1B4_3210 0.48 -0.2 3,593,588 + Pf1N1B4_3210 0.48 +0.6 3,593,589 - Pf1N1B4_3210 0.48 +3.0 3,593,589 - Pf1N1B4_3210 0.48 +1.0 3,593,589 - Pf1N1B4_3210 0.48 -1.0 3,593,646 + Pf1N1B4_3210 0.55 +0.4 3,593,647 - Pf1N1B4_3210 0.55 +1.6 3,593,682 - Pf1N1B4_3210 0.59 -0.0 3,593,826 + Pf1N1B4_3210 0.76 +0.5 3,593,826 + Pf1N1B4_3210 0.76 +1.7 3,593,826 + Pf1N1B4_3210 0.76 +0.4 3,593,826 + Pf1N1B4_3210 0.76 +1.7 3,593,827 - Pf1N1B4_3210 0.76 -0.0 3,593,858 + Pf1N1B4_3210 0.79 -1.3 3,593,858 + Pf1N1B4_3210 0.79 +0.3 3,593,858 + Pf1N1B4_3210 0.79 -0.6 3,593,912 + Pf1N1B4_3210 0.86 +0.5 3,593,912 + -1.0 3,593,912 + +1.6 3,593,912 + Pf1N1B4_3210 0.86 +0.7 3,593,913 - Pf1N1B4_3210 0.86 -1.6 3,593,913 - Pf1N1B4_3210 0.86 -1.0 3,593,913 - Pf1N1B4_3210 0.86 -1.3 3,594,075 + +0.3 3,594,075 + +2.0 3,594,075 + -0.0 3,594,076 - -1.6 3,594,076 - -1.6 3,594,076 - +2.0 3,594,076 - +0.3 3,594,076 - +1.7 3,594,076 - +1.4 3,594,196 + Pf1N1B4_3211 0.45 +1.3 3,594,196 + Pf1N1B4_3211 0.45 -1.0 3,594,196 + Pf1N1B4_3211 0.45 +0.2 3,594,196 + Pf1N1B4_3211 0.45 -2.3 3,594,197 - Pf1N1B4_3211 0.45 +0.1 3,594,197 - Pf1N1B4_3211 0.45 +0.7 3,594,197 - Pf1N1B4_3211 0.45 -0.0 3,594,197 - Pf1N1B4_3211 0.45 +0.5 3,594,197 - +1.0 3,594,197 - Pf1N1B4_3211 0.45 -0.0 3,594,199 + Pf1N1B4_3211 0.46 +0.3 3,594,413 + +1.0 3,594,413 + +0.3 3,594,414 - -1.5 3,594,498 + -0.0 3,594,593 + -0.0 3,594,594 - Pf1N1B4_3212 0.16 -2.1 3,594,594 - Pf1N1B4_3212 0.16 -0.3 3,594,620 + Pf1N1B4_3212 0.20 -0.7 3,594,696 - Pf1N1B4_3212 0.31 +1.4 3,594,785 + Pf1N1B4_3212 0.44 -3.5 3,594,786 - Pf1N1B4_3212 0.44 -1.6 3,594,870 - Pf1N1B4_3212 0.56 -1.6 3,594,870 - Pf1N1B4_3212 0.56 -0.3 3,595,148 - +0.6 3,595,227 - Pf1N1B4_3213 0.29 -0.3 3,595,433 - -0.0 3,595,437 - -2.3 3,595,504 - -0.1 3,595,596 - -0.8 3,595,650 + +1.1 3,595,650 + +0.6 3,595,650 + -0.3 3,595,650 + -0.6 3,595,662 - +0.6 3,595,700 + -1.6 3,595,759 + -1.0 3,595,837 + Pf1N1B4_3214 0.13 +1.0 3,595,837 + +1.0 3,595,837 + Pf1N1B4_3214 0.13 +2.7 3,595,838 - Pf1N1B4_3214 0.13 -0.0 3,595,838 - Pf1N1B4_3214 0.13 -2.6 3,596,122 + Pf1N1B4_3214 0.54 +0.9
Or see this region's nucleotide sequence