Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3165 and Pf1N1B4_3166 are separated by 124 nucleotides Pf1N1B4_3166 and Pf1N1B4_3167 are separated by 17 nucleotides
Pf1N1B4_3165 - DNA polymerase IV (EC 2.7.7.7), at 3,546,965 to 3,548,026
_3165
Pf1N1B4_3166 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 3,548,151 to 3,549,104
_3166
Pf1N1B4_3167 - Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type, at 3,549,122 to 3,550,837
_3167
Position (kb)
3548
3549
3550 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3547.205 kb on + strand, within Pf1N1B4_3165 at 3547.205 kb on + strand, within Pf1N1B4_3165 at 3547.206 kb on - strand, within Pf1N1B4_3165 at 3547.206 kb on - strand, within Pf1N1B4_3165 at 3547.207 kb on + strand, within Pf1N1B4_3165 at 3547.247 kb on + strand, within Pf1N1B4_3165 at 3547.247 kb on + strand, within Pf1N1B4_3165 at 3547.712 kb on - strand at 3547.712 kb on - strand, within Pf1N1B4_3165 at 3547.715 kb on + strand, within Pf1N1B4_3165 at 3547.716 kb on - strand, within Pf1N1B4_3165 at 3547.811 kb on + strand, within Pf1N1B4_3165 at 3547.904 kb on + strand, within Pf1N1B4_3165 at 3547.904 kb on + strand, within Pf1N1B4_3165 at 3547.905 kb on - strand, within Pf1N1B4_3165 at 3548.050 kb on - strand at 3548.055 kb on + strand at 3548.055 kb on + strand at 3548.055 kb on + strand at 3548.055 kb on + strand at 3548.055 kb on + strand at 3548.055 kb on + strand at 3548.055 kb on + strand at 3548.056 kb on - strand at 3548.096 kb on + strand at 3548.096 kb on + strand at 3548.096 kb on + strand at 3548.096 kb on + strand at 3548.097 kb on - strand at 3548.152 kb on + strand at 3548.153 kb on - strand at 3548.316 kb on + strand, within Pf1N1B4_3166 at 3548.317 kb on - strand, within Pf1N1B4_3166 at 3548.353 kb on + strand, within Pf1N1B4_3166 at 3548.353 kb on + strand, within Pf1N1B4_3166 at 3548.353 kb on + strand, within Pf1N1B4_3166 at 3548.353 kb on + strand, within Pf1N1B4_3166 at 3548.353 kb on + strand, within Pf1N1B4_3166 at 3548.353 kb on + strand, within Pf1N1B4_3166 at 3548.354 kb on - strand, within Pf1N1B4_3166 at 3548.354 kb on - strand, within Pf1N1B4_3166 at 3548.354 kb on - strand, within Pf1N1B4_3166 at 3548.354 kb on - strand, within Pf1N1B4_3166 at 3548.354 kb on - strand, within Pf1N1B4_3166 at 3548.354 kb on - strand, within Pf1N1B4_3166 at 3548.354 kb on - strand, within Pf1N1B4_3166 at 3548.354 kb on - strand, within Pf1N1B4_3166 at 3548.606 kb on - strand, within Pf1N1B4_3166 at 3548.606 kb on - strand, within Pf1N1B4_3166 at 3548.606 kb on - strand, within Pf1N1B4_3166 at 3548.710 kb on + strand, within Pf1N1B4_3166 at 3548.710 kb on + strand, within Pf1N1B4_3166 at 3548.710 kb on + strand, within Pf1N1B4_3166 at 3548.710 kb on + strand, within Pf1N1B4_3166 at 3548.710 kb on + strand, within Pf1N1B4_3166 at 3548.711 kb on - strand, within Pf1N1B4_3166 at 3548.711 kb on - strand, within Pf1N1B4_3166 at 3548.711 kb on - strand, within Pf1N1B4_3166 at 3548.711 kb on - strand, within Pf1N1B4_3166 at 3548.711 kb on - strand, within Pf1N1B4_3166 at 3548.711 kb on - strand, within Pf1N1B4_3166 at 3548.821 kb on + strand, within Pf1N1B4_3166 at 3548.821 kb on + strand, within Pf1N1B4_3166 at 3548.821 kb on + strand, within Pf1N1B4_3166 at 3548.821 kb on + strand, within Pf1N1B4_3166 at 3548.821 kb on + strand, within Pf1N1B4_3166 at 3548.821 kb on + strand, within Pf1N1B4_3166 at 3548.821 kb on + strand, within Pf1N1B4_3166 at 3548.821 kb on + strand, within Pf1N1B4_3166 at 3548.822 kb on - strand, within Pf1N1B4_3166 at 3548.822 kb on - strand, within Pf1N1B4_3166 at 3548.822 kb on - strand, within Pf1N1B4_3166 at 3548.822 kb on - strand, within Pf1N1B4_3166 at 3548.822 kb on - strand, within Pf1N1B4_3166 at 3548.822 kb on - strand, within Pf1N1B4_3166 at 3548.822 kb on - strand, within Pf1N1B4_3166 at 3548.822 kb on - strand, within Pf1N1B4_3166 at 3548.822 kb on - strand, within Pf1N1B4_3166 at 3548.822 kb on - strand, within Pf1N1B4_3166 at 3548.822 kb on - strand, within Pf1N1B4_3166 at 3548.822 kb on - strand, within Pf1N1B4_3166 at 3548.843 kb on - strand, within Pf1N1B4_3166 at 3548.852 kb on - strand, within Pf1N1B4_3166 at 3548.852 kb on - strand, within Pf1N1B4_3166 at 3548.852 kb on - strand, within Pf1N1B4_3166 at 3548.860 kb on + strand, within Pf1N1B4_3166 at 3549.084 kb on + strand at 3549.114 kb on - strand at 3549.114 kb on - strand at 3549.114 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 3,547,205 + Pf1N1B4_3165 0.23 -2.3 3,547,205 + Pf1N1B4_3165 0.23 -0.8 3,547,206 - Pf1N1B4_3165 0.23 +0.0 3,547,206 - Pf1N1B4_3165 0.23 +0.0 3,547,207 + Pf1N1B4_3165 0.23 -0.3 3,547,247 + Pf1N1B4_3165 0.27 -0.3 3,547,247 + Pf1N1B4_3165 0.27 -2.3 3,547,712 - +1.6 3,547,712 - Pf1N1B4_3165 0.70 +0.6 3,547,715 + Pf1N1B4_3165 0.71 +0.3 3,547,716 - Pf1N1B4_3165 0.71 -2.0 3,547,811 + Pf1N1B4_3165 0.80 -0.3 3,547,904 + Pf1N1B4_3165 0.88 +0.6 3,547,904 + Pf1N1B4_3165 0.88 +0.7 3,547,905 - Pf1N1B4_3165 0.89 -1.0 3,548,050 - -1.8 3,548,055 + +0.0 3,548,055 + +1.6 3,548,055 + +0.4 3,548,055 + +1.5 3,548,055 + +0.0 3,548,055 + -1.2 3,548,055 + +0.6 3,548,056 - +0.6 3,548,096 + +0.4 3,548,096 + -1.8 3,548,096 + -0.0 3,548,096 + -0.4 3,548,097 - -1.0 3,548,152 + -0.2 3,548,153 - +0.3 3,548,316 + Pf1N1B4_3166 0.17 -1.4 3,548,317 - Pf1N1B4_3166 0.17 -2.0 3,548,353 + Pf1N1B4_3166 0.21 +1.3 3,548,353 + Pf1N1B4_3166 0.21 +1.6 3,548,353 + Pf1N1B4_3166 0.21 -1.0 3,548,353 + Pf1N1B4_3166 0.21 -1.8 3,548,353 + Pf1N1B4_3166 0.21 -1.4 3,548,353 + Pf1N1B4_3166 0.21 -1.6 3,548,354 - Pf1N1B4_3166 0.21 -0.8 3,548,354 - Pf1N1B4_3166 0.21 -0.9 3,548,354 - Pf1N1B4_3166 0.21 +0.2 3,548,354 - Pf1N1B4_3166 0.21 -0.5 3,548,354 - Pf1N1B4_3166 0.21 -2.0 3,548,354 - Pf1N1B4_3166 0.21 -1.0 3,548,354 - Pf1N1B4_3166 0.21 -2.6 3,548,354 - Pf1N1B4_3166 0.21 -2.8 3,548,606 - Pf1N1B4_3166 0.48 -0.6 3,548,606 - Pf1N1B4_3166 0.48 -1.6 3,548,606 - Pf1N1B4_3166 0.48 -1.1 3,548,710 + Pf1N1B4_3166 0.59 -2.0 3,548,710 + Pf1N1B4_3166 0.59 -1.3 3,548,710 + Pf1N1B4_3166 0.59 -1.0 3,548,710 + Pf1N1B4_3166 0.59 -0.4 3,548,710 + Pf1N1B4_3166 0.59 -0.8 3,548,711 - Pf1N1B4_3166 0.59 -1.0 3,548,711 - Pf1N1B4_3166 0.59 -2.8 3,548,711 - Pf1N1B4_3166 0.59 +1.6 3,548,711 - Pf1N1B4_3166 0.59 -0.9 3,548,711 - Pf1N1B4_3166 0.59 -0.4 3,548,711 - Pf1N1B4_3166 0.59 -0.3 3,548,821 + Pf1N1B4_3166 0.70 -1.7 3,548,821 + Pf1N1B4_3166 0.70 -0.5 3,548,821 + Pf1N1B4_3166 0.70 +1.3 3,548,821 + Pf1N1B4_3166 0.70 +0.4 3,548,821 + Pf1N1B4_3166 0.70 -0.4 3,548,821 + Pf1N1B4_3166 0.70 -2.0 3,548,821 + Pf1N1B4_3166 0.70 +0.9 3,548,821 + Pf1N1B4_3166 0.70 -3.0 3,548,822 - Pf1N1B4_3166 0.70 +0.0 3,548,822 - Pf1N1B4_3166 0.70 -0.7 3,548,822 - Pf1N1B4_3166 0.70 -0.7 3,548,822 - Pf1N1B4_3166 0.70 +0.0 3,548,822 - Pf1N1B4_3166 0.70 +0.6 3,548,822 - Pf1N1B4_3166 0.70 +2.0 3,548,822 - Pf1N1B4_3166 0.70 -1.0 3,548,822 - Pf1N1B4_3166 0.70 -0.4 3,548,822 - Pf1N1B4_3166 0.70 +0.5 3,548,822 - Pf1N1B4_3166 0.70 -1.2 3,548,822 - Pf1N1B4_3166 0.70 -0.3 3,548,822 - Pf1N1B4_3166 0.70 -0.3 3,548,843 - Pf1N1B4_3166 0.73 -1.0 3,548,852 - Pf1N1B4_3166 0.73 -1.1 3,548,852 - Pf1N1B4_3166 0.73 -0.6 3,548,852 - Pf1N1B4_3166 0.73 +0.0 3,548,860 + Pf1N1B4_3166 0.74 -1.0 3,549,084 + -0.8 3,549,114 - +2.0 3,549,114 - -0.8 3,549,114 - +1.0
Or see this region's nucleotide sequence