Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3143 and Pf1N1B4_3144 are separated by 53 nucleotides Pf1N1B4_3144 and Pf1N1B4_3145 are separated by 43 nucleotides Pf1N1B4_3145 and Pf1N1B4_3146 are separated by 488 nucleotides Pf1N1B4_3146 and Pf1N1B4_3147 are separated by 0 nucleotides
Pf1N1B4_3143 - RecA protein, at 3,525,750 to 3,526,802
_3143
Pf1N1B4_3144 - Regulatory protein RecX, at 3,526,856 to 3,527,278
_3144
Pf1N1B4_3145 - Decarboxylase family protein, at 3,527,322 to 3,528,440
_3145
Pf1N1B4_3146 - FIG00957354: hypothetical protein, at 3,528,929 to 3,529,123
_3146
Pf1N1B4_3147 - Putative NADPH-quinone reductase (modulator of drug activity B), at 3,529,124 to 3,529,549
_3147
Position (kb)
3527
3528
3529 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3526.398 kb on + strand, within Pf1N1B4_3143 at 3526.398 kb on + strand, within Pf1N1B4_3143 at 3526.551 kb on + strand, within Pf1N1B4_3143 at 3526.551 kb on + strand, within Pf1N1B4_3143 at 3526.552 kb on - strand, within Pf1N1B4_3143 at 3526.617 kb on + strand, within Pf1N1B4_3143 at 3526.617 kb on + strand, within Pf1N1B4_3143 at 3526.822 kb on - strand at 3527.169 kb on + strand, within Pf1N1B4_3144 at 3527.292 kb on + strand at 3527.293 kb on - strand at 3527.293 kb on - strand at 3527.401 kb on + strand at 3527.401 kb on + strand at 3527.401 kb on + strand at 3527.402 kb on - strand at 3527.409 kb on + strand at 3527.410 kb on - strand at 3527.410 kb on - strand at 3527.410 kb on - strand at 3527.410 kb on - strand at 3527.504 kb on - strand, within Pf1N1B4_3145 at 3527.644 kb on + strand, within Pf1N1B4_3145 at 3527.683 kb on + strand, within Pf1N1B4_3145 at 3527.684 kb on - strand at 3527.684 kb on - strand, within Pf1N1B4_3145 at 3527.796 kb on + strand, within Pf1N1B4_3145 at 3527.796 kb on + strand, within Pf1N1B4_3145 at 3528.136 kb on + strand, within Pf1N1B4_3145 at 3528.136 kb on + strand, within Pf1N1B4_3145 at 3528.137 kb on - strand, within Pf1N1B4_3145 at 3528.137 kb on - strand, within Pf1N1B4_3145 at 3528.150 kb on + strand, within Pf1N1B4_3145 at 3528.586 kb on + strand at 3528.587 kb on - strand at 3528.617 kb on + strand at 3528.618 kb on - strand at 3528.618 kb on - strand at 3528.679 kb on + strand at 3528.679 kb on + strand at 3528.743 kb on + strand at 3528.744 kb on - strand at 3528.744 kb on - strand at 3528.791 kb on + strand at 3528.791 kb on + strand at 3528.791 kb on + strand at 3528.792 kb on - strand at 3528.792 kb on - strand at 3528.792 kb on - strand at 3528.792 kb on - strand at 3528.810 kb on + strand at 3528.813 kb on + strand at 3528.813 kb on + strand at 3528.814 kb on - strand at 3528.814 kb on - strand at 3528.814 kb on - strand at 3528.814 kb on - strand at 3528.814 kb on - strand at 3528.818 kb on + strand at 3528.819 kb on - strand at 3528.819 kb on - strand at 3528.965 kb on + strand, within Pf1N1B4_3146 at 3529.058 kb on + strand, within Pf1N1B4_3146 at 3529.126 kb on - strand at 3529.293 kb on + strand, within Pf1N1B4_3147 at 3529.293 kb on + strand, within Pf1N1B4_3147 at 3529.294 kb on - strand, within Pf1N1B4_3147 at 3529.294 kb on - strand, within Pf1N1B4_3147 at 3529.294 kb on - strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.380 kb on + strand, within Pf1N1B4_3147 at 3529.380 kb on + strand, within Pf1N1B4_3147 at 3529.380 kb on + strand, within Pf1N1B4_3147 at 3529.381 kb on - strand, within Pf1N1B4_3147 at 3529.381 kb on - strand, within Pf1N1B4_3147 at 3529.381 kb on - strand, within Pf1N1B4_3147 at 3529.381 kb on - strand, within Pf1N1B4_3147 at 3529.436 kb on + strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 3,526,398 + Pf1N1B4_3143 0.62 +0.0 3,526,398 + Pf1N1B4_3143 0.62 -1.0 3,526,551 + Pf1N1B4_3143 0.76 -3.2 3,526,551 + Pf1N1B4_3143 0.76 +1.2 3,526,552 - Pf1N1B4_3143 0.76 -3.2 3,526,617 + Pf1N1B4_3143 0.82 -2.8 3,526,617 + Pf1N1B4_3143 0.82 +0.0 3,526,822 - +1.0 3,527,169 + Pf1N1B4_3144 0.74 -2.0 3,527,292 + +1.2 3,527,293 - -1.0 3,527,293 - -0.6 3,527,401 + +1.8 3,527,401 + +0.0 3,527,401 + +0.7 3,527,402 - -1.0 3,527,409 + +1.4 3,527,410 - -0.6 3,527,410 - -0.7 3,527,410 - -0.6 3,527,410 - -1.1 3,527,504 - Pf1N1B4_3145 0.16 -0.2 3,527,644 + Pf1N1B4_3145 0.29 -1.7 3,527,683 + Pf1N1B4_3145 0.32 +1.3 3,527,684 - +0.0 3,527,684 - Pf1N1B4_3145 0.32 +0.0 3,527,796 + Pf1N1B4_3145 0.42 -0.7 3,527,796 + Pf1N1B4_3145 0.42 -0.6 3,528,136 + Pf1N1B4_3145 0.73 -1.3 3,528,136 + Pf1N1B4_3145 0.73 +0.0 3,528,137 - Pf1N1B4_3145 0.73 -2.2 3,528,137 - Pf1N1B4_3145 0.73 +0.0 3,528,150 + Pf1N1B4_3145 0.74 -0.6 3,528,586 + -0.3 3,528,587 - +1.6 3,528,617 + +0.3 3,528,618 - -1.3 3,528,618 - +0.4 3,528,679 + -1.0 3,528,679 + +0.2 3,528,743 + +1.6 3,528,744 - +1.3 3,528,744 - -0.6 3,528,791 + +0.7 3,528,791 + -1.6 3,528,791 + +0.0 3,528,792 - +0.4 3,528,792 - -1.0 3,528,792 - -0.2 3,528,792 - +0.2 3,528,810 + +0.5 3,528,813 + -0.3 3,528,813 + +1.2 3,528,814 - -1.0 3,528,814 - -2.8 3,528,814 - +0.2 3,528,814 - +0.6 3,528,814 - +0.2 3,528,818 + +1.4 3,528,819 - +0.9 3,528,819 - -0.6 3,528,965 + Pf1N1B4_3146 0.18 +0.8 3,529,058 + Pf1N1B4_3146 0.66 +0.4 3,529,126 - +1.3 3,529,293 + Pf1N1B4_3147 0.40 +1.4 3,529,293 + Pf1N1B4_3147 0.40 -1.0 3,529,294 - Pf1N1B4_3147 0.40 +2.2 3,529,294 - Pf1N1B4_3147 0.40 +1.0 3,529,294 - Pf1N1B4_3147 0.40 +0.5 3,529,378 + Pf1N1B4_3147 0.60 +0.0 3,529,378 + Pf1N1B4_3147 0.60 +3.2 3,529,378 + Pf1N1B4_3147 0.60 +0.9 3,529,378 + Pf1N1B4_3147 0.60 -0.7 3,529,378 + Pf1N1B4_3147 0.60 +0.5 3,529,378 + Pf1N1B4_3147 0.60 +0.0 3,529,378 + Pf1N1B4_3147 0.60 +0.6 3,529,379 - Pf1N1B4_3147 0.60 +0.9 3,529,379 - Pf1N1B4_3147 0.60 +0.6 3,529,379 - Pf1N1B4_3147 0.60 -0.4 3,529,379 - Pf1N1B4_3147 0.60 -0.2 3,529,379 - Pf1N1B4_3147 0.60 -0.1 3,529,379 - Pf1N1B4_3147 0.60 +0.2 3,529,379 - Pf1N1B4_3147 0.60 +0.6 3,529,379 - Pf1N1B4_3147 0.60 +0.9 3,529,379 - Pf1N1B4_3147 0.60 -0.1 3,529,380 + Pf1N1B4_3147 0.60 +0.0 3,529,380 + Pf1N1B4_3147 0.60 +2.1 3,529,380 + Pf1N1B4_3147 0.60 +0.6 3,529,381 - Pf1N1B4_3147 0.60 +0.4 3,529,381 - Pf1N1B4_3147 0.60 +1.8 3,529,381 - Pf1N1B4_3147 0.60 +0.5 3,529,381 - Pf1N1B4_3147 0.60 +0.6 3,529,436 + Pf1N1B4_3147 0.73 -1.3 3,529,437 - Pf1N1B4_3147 0.73 -1.2 3,529,437 - Pf1N1B4_3147 0.73 +1.7 3,529,437 - Pf1N1B4_3147 0.73 +0.0 3,529,437 - Pf1N1B4_3147 0.73 +1.6 3,529,437 - Pf1N1B4_3147 0.73 +0.6
Or see this region's nucleotide sequence