Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2484 and Pf1N1B4_2485 overlap by 43 nucleotides Pf1N1B4_2485 and Pf1N1B4_2486 are separated by 114 nucleotides Pf1N1B4_2486 and Pf1N1B4_2487 overlap by 33 nucleotides Pf1N1B4_2487 and Pf1N1B4_2488 are separated by 186 nucleotides
Pf1N1B4_2484 - hypothetical protein, at 2,818,947 to 2,819,150
_2484
Pf1N1B4_2485 - Competence protein F homolog, phosphoribosyltransferase domain; protein YhgH required for utilization of DNA as sole source of carbon and energy, at 2,819,108 to 2,819,743
_2485
Pf1N1B4_2486 - hypothetical protein, at 2,819,858 to 2,819,971
_2486
Pf1N1B4_2487 - Biotin synthase (EC 2.8.1.6), at 2,819,939 to 2,820,994
_2487
Pf1N1B4_2488 - 8-amino-7-oxononanoate synthase (EC 2.3.1.47), at 2,821,181 to 2,822,359
_2488
Position (kb)
2819
2820
2821 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2819.033 kb on + strand, within Pf1N1B4_2484 at 2819.034 kb on - strand, within Pf1N1B4_2484 at 2819.104 kb on + strand, within Pf1N1B4_2484 at 2819.104 kb on + strand, within Pf1N1B4_2484 at 2819.104 kb on + strand at 2819.104 kb on + strand, within Pf1N1B4_2484 at 2819.105 kb on - strand, within Pf1N1B4_2484 at 2819.105 kb on - strand, within Pf1N1B4_2484 at 2819.148 kb on + strand at 2819.149 kb on - strand at 2819.604 kb on + strand, within Pf1N1B4_2485 at 2819.604 kb on + strand, within Pf1N1B4_2485 at 2819.604 kb on + strand, within Pf1N1B4_2485 at 2819.605 kb on - strand, within Pf1N1B4_2485 at 2819.605 kb on - strand, within Pf1N1B4_2485 at 2819.744 kb on - strand at 2819.783 kb on + strand at 2819.784 kb on - strand at 2819.784 kb on - strand at 2819.814 kb on + strand at 2819.826 kb on - strand at 2819.859 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.864 kb on - strand at 2819.864 kb on - strand at 2819.894 kb on - strand, within Pf1N1B4_2486 at 2819.922 kb on + strand, within Pf1N1B4_2486 at 2819.923 kb on - strand, within Pf1N1B4_2486 at 2819.923 kb on - strand, within Pf1N1B4_2486 at 2819.923 kb on - strand, within Pf1N1B4_2486 at 2819.923 kb on - strand, within Pf1N1B4_2486 at 2820.131 kb on + strand, within Pf1N1B4_2487 at 2820.132 kb on - strand, within Pf1N1B4_2487 at 2820.152 kb on + strand, within Pf1N1B4_2487 at 2820.152 kb on + strand, within Pf1N1B4_2487 at 2820.152 kb on + strand, within Pf1N1B4_2487 at 2820.153 kb on - strand, within Pf1N1B4_2487 at 2820.153 kb on - strand, within Pf1N1B4_2487 at 2820.153 kb on - strand, within Pf1N1B4_2487 at 2820.153 kb on - strand, within Pf1N1B4_2487 at 2820.290 kb on + strand, within Pf1N1B4_2487 at 2820.291 kb on - strand, within Pf1N1B4_2487 at 2820.401 kb on + strand, within Pf1N1B4_2487 at 2820.401 kb on + strand, within Pf1N1B4_2487 at 2820.402 kb on - strand at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.437 kb on + strand, within Pf1N1B4_2487 at 2820.437 kb on + strand, within Pf1N1B4_2487 at 2820.437 kb on + strand, within Pf1N1B4_2487 at 2820.438 kb on - strand, within Pf1N1B4_2487 at 2820.460 kb on + strand, within Pf1N1B4_2487 at 2820.460 kb on + strand, within Pf1N1B4_2487 at 2820.460 kb on + strand, within Pf1N1B4_2487 at 2820.460 kb on + strand, within Pf1N1B4_2487 at 2820.464 kb on + strand, within Pf1N1B4_2487 at 2820.464 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.492 kb on - strand, within Pf1N1B4_2487 at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.978 kb on - strand at 2820.978 kb on - strand at 2820.978 kb on - strand at 2820.978 kb on - strand at 2820.978 kb on - strand at 2821.002 kb on - strand at 2821.002 kb on - strand at 2821.002 kb on - strand at 2821.138 kb on - strand at 2821.138 kb on - strand at 2821.138 kb on - strand at 2821.138 kb on - strand at 2821.138 kb on - strand at 2821.233 kb on - strand at 2821.309 kb on + strand, within Pf1N1B4_2488 at 2821.309 kb on + strand, within Pf1N1B4_2488 at 2821.309 kb on + strand, within Pf1N1B4_2488 at 2821.319 kb on + strand, within Pf1N1B4_2488 at 2821.320 kb on - strand, within Pf1N1B4_2488 at 2821.499 kb on + strand, within Pf1N1B4_2488 at 2821.500 kb on - strand, within Pf1N1B4_2488 at 2821.500 kb on - strand, within Pf1N1B4_2488 at 2821.500 kb on - strand, within Pf1N1B4_2488 at 2821.500 kb on - strand, within Pf1N1B4_2488 at 2821.679 kb on - strand, within Pf1N1B4_2488 at 2821.964 kb on + strand, within Pf1N1B4_2488 at 2821.964 kb on + strand, within Pf1N1B4_2488 at 2821.964 kb on + strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.970 kb on + strand, within Pf1N1B4_2488 at 2821.970 kb on + strand, within Pf1N1B4_2488 at 2821.970 kb on + strand, within Pf1N1B4_2488 at 2821.970 kb on + strand, within Pf1N1B4_2488 at 2821.970 kb on + strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 2,819,033 + Pf1N1B4_2484 0.42 -0.3 2,819,034 - Pf1N1B4_2484 0.43 -0.5 2,819,104 + Pf1N1B4_2484 0.77 +0.4 2,819,104 + Pf1N1B4_2484 0.77 -1.3 2,819,104 + +0.2 2,819,104 + Pf1N1B4_2484 0.77 +2.2 2,819,105 - Pf1N1B4_2484 0.77 +3.7 2,819,105 - Pf1N1B4_2484 0.77 +0.6 2,819,148 + -0.3 2,819,149 - -0.4 2,819,604 + Pf1N1B4_2485 0.78 -1.8 2,819,604 + Pf1N1B4_2485 0.78 +0.6 2,819,604 + Pf1N1B4_2485 0.78 +1.2 2,819,605 - Pf1N1B4_2485 0.78 -0.5 2,819,605 - Pf1N1B4_2485 0.78 +0.2 2,819,744 - +1.0 2,819,783 + +1.8 2,819,784 - +0.2 2,819,784 - +0.2 2,819,814 + -2.1 2,819,826 - +0.2 2,819,859 + -1.8 2,819,863 + +0.5 2,819,863 + +1.2 2,819,863 + +1.2 2,819,863 + +0.6 2,819,863 + +2.0 2,819,863 + +1.8 2,819,863 + -0.8 2,819,863 + -1.8 2,819,863 + +2.4 2,819,864 - +0.0 2,819,864 - -1.1 2,819,894 - Pf1N1B4_2486 0.32 +0.2 2,819,922 + Pf1N1B4_2486 0.56 +0.5 2,819,923 - Pf1N1B4_2486 0.57 +1.1 2,819,923 - Pf1N1B4_2486 0.57 -0.6 2,819,923 - Pf1N1B4_2486 0.57 -0.8 2,819,923 - Pf1N1B4_2486 0.57 +0.2 2,820,131 + Pf1N1B4_2487 0.18 -0.2 2,820,132 - Pf1N1B4_2487 0.18 -1.2 2,820,152 + Pf1N1B4_2487 0.20 -1.8 2,820,152 + Pf1N1B4_2487 0.20 -0.1 2,820,152 + Pf1N1B4_2487 0.20 +1.1 2,820,153 - Pf1N1B4_2487 0.20 -0.3 2,820,153 - Pf1N1B4_2487 0.20 -0.8 2,820,153 - Pf1N1B4_2487 0.20 +0.4 2,820,153 - Pf1N1B4_2487 0.20 -0.2 2,820,290 + Pf1N1B4_2487 0.33 -0.6 2,820,291 - Pf1N1B4_2487 0.33 -0.2 2,820,401 + Pf1N1B4_2487 0.44 -1.8 2,820,401 + Pf1N1B4_2487 0.44 -0.3 2,820,402 - +0.2 2,820,404 + Pf1N1B4_2487 0.44 -2.3 2,820,404 + Pf1N1B4_2487 0.44 -1.1 2,820,404 + Pf1N1B4_2487 0.44 -0.8 2,820,404 + Pf1N1B4_2487 0.44 -1.6 2,820,404 + Pf1N1B4_2487 0.44 -1.3 2,820,404 + Pf1N1B4_2487 0.44 -0.0 2,820,405 - Pf1N1B4_2487 0.44 +0.2 2,820,405 - Pf1N1B4_2487 0.44 -0.9 2,820,405 - Pf1N1B4_2487 0.44 -0.1 2,820,405 - Pf1N1B4_2487 0.44 -2.9 2,820,405 - Pf1N1B4_2487 0.44 -0.8 2,820,437 + Pf1N1B4_2487 0.47 -3.5 2,820,437 + Pf1N1B4_2487 0.47 +0.2 2,820,437 + Pf1N1B4_2487 0.47 -1.6 2,820,438 - Pf1N1B4_2487 0.47 -0.6 2,820,460 + Pf1N1B4_2487 0.49 -1.8 2,820,460 + Pf1N1B4_2487 0.49 -2.3 2,820,460 + Pf1N1B4_2487 0.49 +0.2 2,820,460 + Pf1N1B4_2487 0.49 -1.3 2,820,464 + Pf1N1B4_2487 0.50 -0.8 2,820,464 + Pf1N1B4_2487 0.50 +1.2 2,820,491 + Pf1N1B4_2487 0.52 +0.2 2,820,491 + Pf1N1B4_2487 0.52 +0.8 2,820,491 + Pf1N1B4_2487 0.52 -0.9 2,820,491 + Pf1N1B4_2487 0.52 -1.8 2,820,491 + Pf1N1B4_2487 0.52 -1.1 2,820,491 + Pf1N1B4_2487 0.52 -0.5 2,820,491 + Pf1N1B4_2487 0.52 +0.2 2,820,492 - Pf1N1B4_2487 0.52 -2.3 2,820,977 + -1.3 2,820,977 + +0.2 2,820,977 + +0.8 2,820,977 + +2.6 2,820,977 + +0.8 2,820,977 + +0.2 2,820,977 + -0.3 2,820,978 - +0.2 2,820,978 - -2.3 2,820,978 - +0.5 2,820,978 - +0.2 2,820,978 - +0.0 2,821,002 - +0.5 2,821,002 - -0.6 2,821,002 - -0.8 2,821,138 - -0.3 2,821,138 - +0.8 2,821,138 - -1.3 2,821,138 - -1.1 2,821,138 - +0.8 2,821,233 - -2.5 2,821,309 + Pf1N1B4_2488 0.11 -1.6 2,821,309 + Pf1N1B4_2488 0.11 -0.8 2,821,309 + Pf1N1B4_2488 0.11 -2.8 2,821,319 + Pf1N1B4_2488 0.12 -1.8 2,821,320 - Pf1N1B4_2488 0.12 +2.1 2,821,499 + Pf1N1B4_2488 0.27 +0.6 2,821,500 - Pf1N1B4_2488 0.27 -0.8 2,821,500 - Pf1N1B4_2488 0.27 -1.8 2,821,500 - Pf1N1B4_2488 0.27 -1.0 2,821,500 - Pf1N1B4_2488 0.27 -0.3 2,821,679 - Pf1N1B4_2488 0.42 +0.2 2,821,964 + Pf1N1B4_2488 0.66 -0.5 2,821,964 + Pf1N1B4_2488 0.66 +0.7 2,821,964 + Pf1N1B4_2488 0.66 -1.9 2,821,965 - Pf1N1B4_2488 0.66 +0.6 2,821,965 - Pf1N1B4_2488 0.66 -0.4 2,821,965 - Pf1N1B4_2488 0.66 +0.5 2,821,965 - Pf1N1B4_2488 0.66 -2.1 2,821,965 - Pf1N1B4_2488 0.66 -0.8 2,821,965 - Pf1N1B4_2488 0.66 +1.2 2,821,970 + Pf1N1B4_2488 0.67 -0.8 2,821,970 + Pf1N1B4_2488 0.67 +1.8 2,821,970 + Pf1N1B4_2488 0.67 -0.5 2,821,970 + Pf1N1B4_2488 0.67 +1.2 2,821,970 + Pf1N1B4_2488 0.67 -3.6 2,821,971 - Pf1N1B4_2488 0.67 +1.4 2,821,971 - Pf1N1B4_2488 0.67 -2.6 2,821,971 - Pf1N1B4_2488 0.67 -2.6 2,821,971 - Pf1N1B4_2488 0.67 -0.8 2,821,971 - Pf1N1B4_2488 0.67 -1.0 2,821,971 - Pf1N1B4_2488 0.67 +1.6 2,821,971 - Pf1N1B4_2488 0.67 -2.1
Or see this region's nucleotide sequence