Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1991 and Pf1N1B4_1992 overlap by 4 nucleotides Pf1N1B4_1992 and Pf1N1B4_1993 are separated by 127 nucleotides Pf1N1B4_1993 and Pf1N1B4_1994 are separated by 28 nucleotides
Pf1N1B4_1991 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 2,276,193 to 2,277,401
_1991
Pf1N1B4_1992 - Glutamine synthetase adenylyltransferase, at 2,277,398 to 2,277,775
_1992
Pf1N1B4_1993 - C4-type zinc finger protein, DksA/TraR family, at 2,277,903 to 2,278,307
_1993
Pf1N1B4_1994 - Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family, at 2,278,336 to 2,279,544
_1994
Position (kb)
2277
2278 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2276.426 kb on - strand, within Pf1N1B4_1991 at 2276.438 kb on + strand, within Pf1N1B4_1991 at 2276.439 kb on - strand, within Pf1N1B4_1991 at 2276.941 kb on - strand, within Pf1N1B4_1991 at 2277.196 kb on + strand, within Pf1N1B4_1991 at 2277.360 kb on + strand at 2277.360 kb on + strand at 2277.360 kb on + strand at 2277.361 kb on - strand at 2277.361 kb on - strand at 2277.493 kb on - strand, within Pf1N1B4_1992 at 2277.493 kb on - strand, within Pf1N1B4_1992 at 2277.588 kb on - strand, within Pf1N1B4_1992 at 2277.606 kb on + strand, within Pf1N1B4_1992 at 2277.606 kb on + strand, within Pf1N1B4_1992 at 2277.606 kb on + strand, within Pf1N1B4_1992 at 2277.606 kb on + strand, within Pf1N1B4_1992 at 2277.606 kb on + strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.636 kb on + strand, within Pf1N1B4_1992 at 2277.636 kb on + strand, within Pf1N1B4_1992 at 2277.656 kb on + strand, within Pf1N1B4_1992 at 2277.656 kb on + strand, within Pf1N1B4_1992 at 2277.656 kb on + strand, within Pf1N1B4_1992 at 2277.657 kb on - strand, within Pf1N1B4_1992 at 2277.657 kb on - strand, within Pf1N1B4_1992 at 2277.657 kb on - strand, within Pf1N1B4_1992 at 2277.657 kb on - strand, within Pf1N1B4_1992 at 2277.657 kb on - strand, within Pf1N1B4_1992 at 2277.802 kb on + strand at 2277.802 kb on + strand at 2277.803 kb on - strand at 2277.803 kb on - strand at 2277.828 kb on + strand at 2277.829 kb on - strand at 2277.942 kb on + strand at 2277.942 kb on + strand at 2277.943 kb on - strand at 2277.943 kb on - strand at 2277.943 kb on - strand at 2277.943 kb on - strand at 2278.253 kb on + strand, within Pf1N1B4_1993 at 2278.253 kb on + strand, within Pf1N1B4_1993 at 2278.330 kb on + strand at 2278.331 kb on - strand at 2278.331 kb on - strand at 2278.393 kb on - strand at 2278.401 kb on + strand at 2278.401 kb on + strand at 2278.402 kb on - strand at 2278.405 kb on + strand at 2278.405 kb on + strand at 2278.405 kb on + strand at 2278.405 kb on + strand at 2278.410 kb on - strand at 2278.413 kb on - strand at 2278.413 kb on - strand at 2278.712 kb on + strand, within Pf1N1B4_1994 at 2278.712 kb on + strand, within Pf1N1B4_1994 at 2278.712 kb on + strand, within Pf1N1B4_1994 at 2278.713 kb on - strand, within Pf1N1B4_1994 at 2278.721 kb on + strand, within Pf1N1B4_1994 at 2278.769 kb on - strand, within Pf1N1B4_1994 at 2278.769 kb on - strand, within Pf1N1B4_1994
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 2,276,426 - Pf1N1B4_1991 0.19 -2.8 2,276,438 + Pf1N1B4_1991 0.20 -1.2 2,276,439 - Pf1N1B4_1991 0.20 +0.4 2,276,941 - Pf1N1B4_1991 0.62 +1.4 2,277,196 + Pf1N1B4_1991 0.83 -1.1 2,277,360 + -1.2 2,277,360 + +1.4 2,277,360 + -0.3 2,277,361 - +0.7 2,277,361 - -0.0 2,277,493 - Pf1N1B4_1992 0.25 +0.4 2,277,493 - Pf1N1B4_1992 0.25 -0.6 2,277,588 - Pf1N1B4_1992 0.50 -0.8 2,277,606 + Pf1N1B4_1992 0.55 -1.6 2,277,606 + Pf1N1B4_1992 0.55 +2.4 2,277,606 + Pf1N1B4_1992 0.55 -0.0 2,277,606 + Pf1N1B4_1992 0.55 +0.1 2,277,606 + Pf1N1B4_1992 0.55 -1.6 2,277,607 - Pf1N1B4_1992 0.55 +0.4 2,277,607 - Pf1N1B4_1992 0.55 -0.2 2,277,607 - Pf1N1B4_1992 0.55 -0.8 2,277,607 - Pf1N1B4_1992 0.55 +1.0 2,277,607 - Pf1N1B4_1992 0.55 +1.0 2,277,607 - Pf1N1B4_1992 0.55 -0.6 2,277,607 - Pf1N1B4_1992 0.55 +0.2 2,277,607 - Pf1N1B4_1992 0.55 -1.6 2,277,607 - Pf1N1B4_1992 0.55 +0.4 2,277,607 - Pf1N1B4_1992 0.55 +1.9 2,277,636 + Pf1N1B4_1992 0.63 -0.1 2,277,636 + Pf1N1B4_1992 0.63 +1.7 2,277,656 + Pf1N1B4_1992 0.68 -1.6 2,277,656 + Pf1N1B4_1992 0.68 +2.0 2,277,656 + Pf1N1B4_1992 0.68 -1.6 2,277,657 - Pf1N1B4_1992 0.69 -0.2 2,277,657 - Pf1N1B4_1992 0.69 -1.6 2,277,657 - Pf1N1B4_1992 0.69 +0.4 2,277,657 - Pf1N1B4_1992 0.69 +0.6 2,277,657 - Pf1N1B4_1992 0.69 +2.4 2,277,802 + +1.1 2,277,802 + +1.0 2,277,803 - +0.4 2,277,803 - -0.6 2,277,828 + +0.1 2,277,829 - -0.5 2,277,942 + -0.0 2,277,942 + -1.9 2,277,943 - -2.2 2,277,943 - +0.7 2,277,943 - +1.0 2,277,943 - +0.4 2,278,253 + Pf1N1B4_1993 0.86 +0.9 2,278,253 + Pf1N1B4_1993 0.86 +1.6 2,278,330 + -0.3 2,278,331 - -0.1 2,278,331 - +0.4 2,278,393 - -1.6 2,278,401 + +2.0 2,278,401 + +0.2 2,278,402 - -1.3 2,278,405 + +0.8 2,278,405 + -0.6 2,278,405 + +1.2 2,278,405 + -0.2 2,278,410 - -0.6 2,278,413 - -0.9 2,278,413 - -0.8 2,278,712 + Pf1N1B4_1994 0.31 +2.5 2,278,712 + Pf1N1B4_1994 0.31 +1.0 2,278,712 + Pf1N1B4_1994 0.31 -1.0 2,278,713 - Pf1N1B4_1994 0.31 +0.1 2,278,721 + Pf1N1B4_1994 0.32 +1.7 2,278,769 - Pf1N1B4_1994 0.36 -0.0 2,278,769 - Pf1N1B4_1994 0.36 +2.7
Or see this region's nucleotide sequence