Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_196 and Pf1N1B4_197 overlap by 4 nucleotides Pf1N1B4_197 and Pf1N1B4_198 overlap by 4 nucleotides Pf1N1B4_198 and Pf1N1B4_199 overlap by 4 nucleotides Pf1N1B4_199 and Pf1N1B4_200 are separated by 35 nucleotides
Pf1N1B4_196 - FIG137594: Putative iron-regulated membrane protein, at 244,571 to 245,776
_196
Pf1N1B4_197 - FIG139991: Putative thiamine pyrophosphate-requiring enzyme, at 245,773 to 246,315
_197
Pf1N1B4_198 - FIG137877: Hypothetical protein in pyoverdin gene cluster, at 246,312 to 246,632
_198
Pf1N1B4_199 - FIG049111: Hypothetical protein in pyoverdin gene cluster, at 246,629 to 247,204
_199
Pf1N1B4_200 - FIG014801: Cation ABC transporter, periplasmic cation-binding protein, at 247,240 to 248,169
_200
Position (kb) 245
246
247 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 244.889 kb on + strand, within Pf1N1B4_196 at 244.889 kb on + strand, within Pf1N1B4_196 at 244.889 kb on + strand, within Pf1N1B4_196 at 244.890 kb on - strand at 244.890 kb on - strand, within Pf1N1B4_196 at 244.890 kb on - strand, within Pf1N1B4_196 at 245.255 kb on - strand, within Pf1N1B4_196 at 245.414 kb on + strand, within Pf1N1B4_196 at 245.414 kb on + strand, within Pf1N1B4_196 at 245.414 kb on + strand, within Pf1N1B4_196 at 245.483 kb on + strand, within Pf1N1B4_196 at 245.483 kb on + strand, within Pf1N1B4_196 at 245.484 kb on - strand, within Pf1N1B4_196 at 245.887 kb on + strand, within Pf1N1B4_197 at 245.888 kb on - strand, within Pf1N1B4_197 at 245.888 kb on - strand, within Pf1N1B4_197 at 246.025 kb on + strand, within Pf1N1B4_197 at 246.025 kb on + strand at 246.337 kb on + strand at 246.338 kb on - strand at 246.338 kb on - strand at 246.338 kb on - strand at 246.341 kb on - strand at 246.564 kb on + strand, within Pf1N1B4_198 at 246.564 kb on + strand, within Pf1N1B4_198 at 246.564 kb on + strand at 246.564 kb on + strand, within Pf1N1B4_198 at 246.565 kb on - strand, within Pf1N1B4_198 at 246.565 kb on - strand, within Pf1N1B4_198 at 246.565 kb on - strand, within Pf1N1B4_198 at 246.565 kb on - strand, within Pf1N1B4_198 at 246.634 kb on + strand at 246.634 kb on + strand at 246.635 kb on - strand at 246.635 kb on - strand at 246.635 kb on - strand at 246.686 kb on + strand at 246.686 kb on + strand at 246.686 kb on + strand at 246.686 kb on + strand at 246.686 kb on + strand at 246.686 kb on + strand at 246.686 kb on + strand at 246.687 kb on - strand, within Pf1N1B4_199 at 246.687 kb on - strand, within Pf1N1B4_199 at 246.687 kb on - strand, within Pf1N1B4_199 at 246.687 kb on - strand, within Pf1N1B4_199 at 246.687 kb on - strand, within Pf1N1B4_199 at 246.687 kb on - strand, within Pf1N1B4_199 at 246.687 kb on - strand, within Pf1N1B4_199 at 246.824 kb on - strand, within Pf1N1B4_199 at 246.824 kb on - strand, within Pf1N1B4_199 at 246.824 kb on - strand, within Pf1N1B4_199 at 246.824 kb on - strand at 246.824 kb on - strand, within Pf1N1B4_199 at 246.824 kb on - strand, within Pf1N1B4_199 at 246.828 kb on - strand, within Pf1N1B4_199 at 246.828 kb on - strand, within Pf1N1B4_199 at 246.996 kb on - strand, within Pf1N1B4_199 at 246.996 kb on - strand, within Pf1N1B4_199 at 247.245 kb on + strand at 247.246 kb on - strand at 247.246 kb on - strand at 247.246 kb on - strand at 247.246 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 244,889 + Pf1N1B4_196 0.26 -1.9 244,889 + Pf1N1B4_196 0.26 +1.4 244,889 + Pf1N1B4_196 0.26 +0.4 244,890 - -0.2 244,890 - Pf1N1B4_196 0.26 -0.2 244,890 - Pf1N1B4_196 0.26 -0.6 245,255 - Pf1N1B4_196 0.57 -0.6 245,414 + Pf1N1B4_196 0.70 -0.2 245,414 + Pf1N1B4_196 0.70 +0.6 245,414 + Pf1N1B4_196 0.70 -1.2 245,483 + Pf1N1B4_196 0.76 +0.5 245,483 + Pf1N1B4_196 0.76 -3.9 245,484 - Pf1N1B4_196 0.76 -1.8 245,887 + Pf1N1B4_197 0.21 +2.0 245,888 - Pf1N1B4_197 0.21 +0.1 245,888 - Pf1N1B4_197 0.21 -1.4 246,025 + Pf1N1B4_197 0.46 +3.0 246,025 + +0.4 246,337 + +0.2 246,338 - -0.9 246,338 - +0.5 246,338 - +0.8 246,341 - +0.6 246,564 + Pf1N1B4_198 0.79 +0.9 246,564 + Pf1N1B4_198 0.79 +0.4 246,564 + +2.1 246,564 + Pf1N1B4_198 0.79 -2.2 246,565 - Pf1N1B4_198 0.79 +0.0 246,565 - Pf1N1B4_198 0.79 -0.8 246,565 - Pf1N1B4_198 0.79 -2.2 246,565 - Pf1N1B4_198 0.79 -1.8 246,634 + -1.5 246,634 + +0.2 246,635 - -0.2 246,635 - -0.2 246,635 - +0.4 246,686 + +0.1 246,686 + -0.2 246,686 + +1.1 246,686 + +0.2 246,686 + -0.2 246,686 + -1.5 246,686 + -0.2 246,687 - Pf1N1B4_199 0.10 -1.8 246,687 - Pf1N1B4_199 0.10 +0.3 246,687 - Pf1N1B4_199 0.10 -0.2 246,687 - Pf1N1B4_199 0.10 +0.1 246,687 - Pf1N1B4_199 0.10 +0.2 246,687 - Pf1N1B4_199 0.10 +0.9 246,687 - Pf1N1B4_199 0.10 +0.1 246,824 - Pf1N1B4_199 0.34 +1.8 246,824 - Pf1N1B4_199 0.34 -1.8 246,824 - Pf1N1B4_199 0.34 +0.2 246,824 - -0.2 246,824 - Pf1N1B4_199 0.34 +0.4 246,824 - Pf1N1B4_199 0.34 -0.2 246,828 - Pf1N1B4_199 0.35 -0.6 246,828 - Pf1N1B4_199 0.35 -1.2 246,996 - Pf1N1B4_199 0.64 +1.0 246,996 - Pf1N1B4_199 0.64 +0.2 247,245 + -0.8 247,246 - -1.8 247,246 - +0.8 247,246 - +0.4 247,246 - +1.4
Or see this region's nucleotide sequence