Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1914 and Pf1N1B4_1915 are separated by 22 nucleotides Pf1N1B4_1915 and Pf1N1B4_1916 are separated by 131 nucleotides Pf1N1B4_1916 and Pf1N1B4_1917 are separated by 102 nucleotides
Pf1N1B4_1914 - Ribosomal RNA small subunit methyltransferase B (EC 2.1.1.-), at 2,188,260 to 2,189,570
_1914
Pf1N1B4_1915 - Trk system potassium uptake protein TrkA, at 2,189,593 to 2,190,966
_1915
Pf1N1B4_1916 - Chaperone protein YscY (Yop proteins translocation protein Y), at 2,191,098 to 2,191,412
_1916
Pf1N1B4_1917 - Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-), at 2,191,515 to 2,192,402
_1917
Position (kb)
2189
2190
2191 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2188.812 kb on + strand, within Pf1N1B4_1914 at 2188.813 kb on - strand, within Pf1N1B4_1914 at 2188.813 kb on - strand, within Pf1N1B4_1914 at 2188.813 kb on - strand, within Pf1N1B4_1914 at 2188.813 kb on - strand, within Pf1N1B4_1914 at 2188.813 kb on - strand, within Pf1N1B4_1914 at 2188.813 kb on - strand, within Pf1N1B4_1914 at 2188.860 kb on + strand, within Pf1N1B4_1914 at 2188.860 kb on + strand, within Pf1N1B4_1914 at 2188.861 kb on - strand, within Pf1N1B4_1914 at 2188.877 kb on + strand, within Pf1N1B4_1914 at 2188.877 kb on + strand, within Pf1N1B4_1914 at 2189.330 kb on + strand, within Pf1N1B4_1914 at 2189.331 kb on - strand, within Pf1N1B4_1914 at 2189.331 kb on - strand, within Pf1N1B4_1914 at 2189.331 kb on - strand, within Pf1N1B4_1914 at 2189.331 kb on - strand, within Pf1N1B4_1914 at 2189.331 kb on - strand, within Pf1N1B4_1914 at 2189.368 kb on - strand, within Pf1N1B4_1914 at 2189.497 kb on + strand at 2189.497 kb on + strand at 2189.529 kb on + strand at 2189.530 kb on - strand at 2189.532 kb on + strand at 2189.578 kb on + strand at 2189.579 kb on - strand at 2189.903 kb on - strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.983 kb on + strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2189.984 kb on - strand, within Pf1N1B4_1915 at 2190.023 kb on + strand, within Pf1N1B4_1915 at 2190.023 kb on + strand, within Pf1N1B4_1915 at 2190.023 kb on + strand, within Pf1N1B4_1915 at 2190.023 kb on + strand, within Pf1N1B4_1915 at 2190.023 kb on + strand, within Pf1N1B4_1915 at 2190.024 kb on - strand, within Pf1N1B4_1915 at 2190.070 kb on + strand, within Pf1N1B4_1915 at 2190.070 kb on + strand, within Pf1N1B4_1915 at 2190.071 kb on - strand, within Pf1N1B4_1915 at 2190.071 kb on - strand, within Pf1N1B4_1915 at 2190.071 kb on - strand, within Pf1N1B4_1915 at 2190.071 kb on - strand, within Pf1N1B4_1915 at 2190.071 kb on - strand, within Pf1N1B4_1915 at 2190.074 kb on - strand, within Pf1N1B4_1915 at 2190.215 kb on + strand, within Pf1N1B4_1915 at 2190.215 kb on + strand, within Pf1N1B4_1915 at 2190.215 kb on + strand, within Pf1N1B4_1915 at 2190.240 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand, within Pf1N1B4_1915 at 2190.283 kb on + strand at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.284 kb on - strand, within Pf1N1B4_1915 at 2190.291 kb on + strand, within Pf1N1B4_1915 at 2190.291 kb on + strand, within Pf1N1B4_1915 at 2190.291 kb on + strand, within Pf1N1B4_1915 at 2190.291 kb on + strand, within Pf1N1B4_1915 at 2190.291 kb on + strand, within Pf1N1B4_1915 at 2190.292 kb on - strand, within Pf1N1B4_1915 at 2190.292 kb on - strand, within Pf1N1B4_1915 at 2190.292 kb on - strand, within Pf1N1B4_1915 at 2190.292 kb on - strand, within Pf1N1B4_1915 at 2190.292 kb on - strand, within Pf1N1B4_1915 at 2190.360 kb on - strand, within Pf1N1B4_1915 at 2190.592 kb on + strand, within Pf1N1B4_1915 at 2190.592 kb on + strand, within Pf1N1B4_1915 at 2190.593 kb on - strand, within Pf1N1B4_1915 at 2190.593 kb on - strand, within Pf1N1B4_1915 at 2190.593 kb on - strand, within Pf1N1B4_1915 at 2190.593 kb on - strand, within Pf1N1B4_1915 at 2190.909 kb on + strand at 2190.918 kb on + strand at 2190.918 kb on + strand at 2191.173 kb on + strand, within Pf1N1B4_1916 at 2191.242 kb on + strand, within Pf1N1B4_1916 at 2191.243 kb on - strand, within Pf1N1B4_1916 at 2191.243 kb on - strand, within Pf1N1B4_1916 at 2191.262 kb on - strand, within Pf1N1B4_1916 at 2191.407 kb on + strand at 2191.407 kb on + strand at 2191.407 kb on + strand at 2191.408 kb on - strand at 2191.408 kb on - strand at 2191.420 kb on + strand at 2191.420 kb on + strand at 2191.420 kb on + strand at 2191.420 kb on + strand at 2191.420 kb on + strand at 2191.420 kb on + strand at 2191.421 kb on - strand at 2191.421 kb on - strand at 2191.464 kb on + strand at 2191.520 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 2,188,812 + Pf1N1B4_1914 0.42 +0.2 2,188,813 - Pf1N1B4_1914 0.42 -0.2 2,188,813 - Pf1N1B4_1914 0.42 -2.4 2,188,813 - Pf1N1B4_1914 0.42 -1.2 2,188,813 - Pf1N1B4_1914 0.42 +0.4 2,188,813 - Pf1N1B4_1914 0.42 -1.6 2,188,813 - Pf1N1B4_1914 0.42 -0.2 2,188,860 + Pf1N1B4_1914 0.46 -1.6 2,188,860 + Pf1N1B4_1914 0.46 -1.5 2,188,861 - Pf1N1B4_1914 0.46 +0.4 2,188,877 + Pf1N1B4_1914 0.47 +0.0 2,188,877 + Pf1N1B4_1914 0.47 -0.6 2,189,330 + Pf1N1B4_1914 0.82 -2.0 2,189,331 - Pf1N1B4_1914 0.82 -1.9 2,189,331 - Pf1N1B4_1914 0.82 -0.2 2,189,331 - Pf1N1B4_1914 0.82 -0.7 2,189,331 - Pf1N1B4_1914 0.82 +0.0 2,189,331 - Pf1N1B4_1914 0.82 +1.0 2,189,368 - Pf1N1B4_1914 0.85 -0.2 2,189,497 + -0.0 2,189,497 + +2.7 2,189,529 + +2.0 2,189,530 - -1.2 2,189,532 + +0.4 2,189,578 + +2.0 2,189,579 - +0.9 2,189,903 - Pf1N1B4_1915 0.23 +2.0 2,189,983 + Pf1N1B4_1915 0.28 +0.3 2,189,983 + Pf1N1B4_1915 0.28 -0.9 2,189,983 + Pf1N1B4_1915 0.28 +0.4 2,189,983 + Pf1N1B4_1915 0.28 -0.6 2,189,983 + Pf1N1B4_1915 0.28 -0.2 2,189,983 + Pf1N1B4_1915 0.28 -0.3 2,189,983 + Pf1N1B4_1915 0.28 -2.4 2,189,983 + Pf1N1B4_1915 0.28 -2.4 2,189,983 + Pf1N1B4_1915 0.28 -1.0 2,189,983 + Pf1N1B4_1915 0.28 -0.4 2,189,983 + Pf1N1B4_1915 0.28 -1.2 2,189,983 + Pf1N1B4_1915 0.28 -0.9 2,189,983 + Pf1N1B4_1915 0.28 +0.4 2,189,984 - Pf1N1B4_1915 0.28 -0.4 2,189,984 - Pf1N1B4_1915 0.28 +0.1 2,189,984 - Pf1N1B4_1915 0.28 -0.2 2,189,984 - Pf1N1B4_1915 0.28 -0.7 2,189,984 - Pf1N1B4_1915 0.28 -2.0 2,189,984 - Pf1N1B4_1915 0.28 -0.6 2,189,984 - Pf1N1B4_1915 0.28 +1.3 2,189,984 - Pf1N1B4_1915 0.28 -2.4 2,189,984 - Pf1N1B4_1915 0.28 -0.6 2,189,984 - Pf1N1B4_1915 0.28 -1.2 2,189,984 - Pf1N1B4_1915 0.28 -2.3 2,189,984 - Pf1N1B4_1915 0.28 -1.4 2,189,984 - Pf1N1B4_1915 0.28 -1.6 2,189,984 - Pf1N1B4_1915 0.28 +0.1 2,189,984 - Pf1N1B4_1915 0.28 +1.2 2,189,984 - Pf1N1B4_1915 0.28 +0.4 2,190,023 + Pf1N1B4_1915 0.31 -3.0 2,190,023 + Pf1N1B4_1915 0.31 -1.6 2,190,023 + Pf1N1B4_1915 0.31 +0.4 2,190,023 + Pf1N1B4_1915 0.31 -0.2 2,190,023 + Pf1N1B4_1915 0.31 -1.2 2,190,024 - Pf1N1B4_1915 0.31 -2.6 2,190,070 + Pf1N1B4_1915 0.35 -3.2 2,190,070 + Pf1N1B4_1915 0.35 +1.4 2,190,071 - Pf1N1B4_1915 0.35 -1.6 2,190,071 - Pf1N1B4_1915 0.35 +2.4 2,190,071 - Pf1N1B4_1915 0.35 +1.0 2,190,071 - Pf1N1B4_1915 0.35 -1.1 2,190,071 - Pf1N1B4_1915 0.35 -0.9 2,190,074 - Pf1N1B4_1915 0.35 -0.4 2,190,215 + Pf1N1B4_1915 0.45 -1.2 2,190,215 + Pf1N1B4_1915 0.45 -1.2 2,190,215 + Pf1N1B4_1915 0.45 -2.6 2,190,240 + Pf1N1B4_1915 0.47 +0.4 2,190,283 + Pf1N1B4_1915 0.50 -1.0 2,190,283 + Pf1N1B4_1915 0.50 -2.0 2,190,283 + Pf1N1B4_1915 0.50 -1.3 2,190,283 + Pf1N1B4_1915 0.50 -2.2 2,190,283 + Pf1N1B4_1915 0.50 -2.2 2,190,283 + Pf1N1B4_1915 0.50 -2.4 2,190,283 + Pf1N1B4_1915 0.50 +0.4 2,190,283 + Pf1N1B4_1915 0.50 +0.4 2,190,283 + Pf1N1B4_1915 0.50 +0.4 2,190,283 + -1.2 2,190,284 - Pf1N1B4_1915 0.50 -0.3 2,190,284 - Pf1N1B4_1915 0.50 -1.2 2,190,284 - Pf1N1B4_1915 0.50 -1.3 2,190,284 - Pf1N1B4_1915 0.50 -0.2 2,190,284 - Pf1N1B4_1915 0.50 +0.8 2,190,284 - Pf1N1B4_1915 0.50 -0.2 2,190,284 - Pf1N1B4_1915 0.50 -0.3 2,190,284 - Pf1N1B4_1915 0.50 -2.6 2,190,284 - Pf1N1B4_1915 0.50 -1.2 2,190,284 - Pf1N1B4_1915 0.50 -0.1 2,190,291 + Pf1N1B4_1915 0.51 -0.8 2,190,291 + Pf1N1B4_1915 0.51 -0.4 2,190,291 + Pf1N1B4_1915 0.51 -0.1 2,190,291 + Pf1N1B4_1915 0.51 -0.6 2,190,291 + Pf1N1B4_1915 0.51 +1.4 2,190,292 - Pf1N1B4_1915 0.51 -0.7 2,190,292 - Pf1N1B4_1915 0.51 -2.8 2,190,292 - Pf1N1B4_1915 0.51 +0.2 2,190,292 - Pf1N1B4_1915 0.51 -1.8 2,190,292 - Pf1N1B4_1915 0.51 -0.3 2,190,360 - Pf1N1B4_1915 0.56 -1.0 2,190,592 + Pf1N1B4_1915 0.73 -1.4 2,190,592 + Pf1N1B4_1915 0.73 -0.2 2,190,593 - Pf1N1B4_1915 0.73 -0.8 2,190,593 - Pf1N1B4_1915 0.73 +0.4 2,190,593 - Pf1N1B4_1915 0.73 -0.8 2,190,593 - Pf1N1B4_1915 0.73 -0.9 2,190,909 + -0.4 2,190,918 + -0.9 2,190,918 + -0.6 2,191,173 + Pf1N1B4_1916 0.24 -0.9 2,191,242 + Pf1N1B4_1916 0.46 +1.4 2,191,243 - Pf1N1B4_1916 0.46 -2.2 2,191,243 - Pf1N1B4_1916 0.46 +0.0 2,191,262 - Pf1N1B4_1916 0.52 -0.6 2,191,407 + +0.7 2,191,407 + -1.2 2,191,407 + +1.6 2,191,408 - -2.6 2,191,408 - +2.2 2,191,420 + -1.9 2,191,420 + +2.4 2,191,420 + -0.2 2,191,420 + +0.4 2,191,420 + -0.3 2,191,420 + +0.4 2,191,421 - +0.2 2,191,421 - +2.4 2,191,464 + -2.6 2,191,520 - -1.6
Or see this region's nucleotide sequence