Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1785 and Pf1N1B4_1786 are separated by 422 nucleotides Pf1N1B4_1786 and Pf1N1B4_1787 are separated by 228 nucleotides
Pf1N1B4_1785 - Uricase (urate oxidase) (EC 1.7.3.3), at 2,037,423 to 2,037,917
_1785
Pf1N1B4_1786 - ATP-dependent DNA helicase, RecQ family, at 2,038,340 to 2,040,274
_1786
Pf1N1B4_1787 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 2,040,503 to 2,041,729
_1787
Position (kb)
2038
2039
2040
2041 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2037.377 kb on + strand at 2037.418 kb on + strand at 2037.418 kb on + strand at 2037.418 kb on + strand at 2037.418 kb on + strand at 2037.418 kb on + strand at 2037.418 kb on + strand at 2037.418 kb on + strand at 2037.419 kb on - strand at 2037.419 kb on - strand at 2037.419 kb on - strand at 2037.419 kb on - strand at 2037.423 kb on - strand at 2037.423 kb on - strand at 2037.433 kb on + strand at 2037.434 kb on - strand at 2037.437 kb on + strand at 2037.437 kb on + strand at 2037.437 kb on + strand at 2037.437 kb on + strand at 2037.438 kb on - strand at 2037.438 kb on - strand at 2037.438 kb on - strand at 2037.593 kb on - strand, within Pf1N1B4_1785 at 2037.723 kb on + strand, within Pf1N1B4_1785 at 2037.723 kb on + strand, within Pf1N1B4_1785 at 2037.723 kb on + strand, within Pf1N1B4_1785 at 2037.723 kb on + strand, within Pf1N1B4_1785 at 2037.723 kb on + strand, within Pf1N1B4_1785 at 2037.723 kb on + strand, within Pf1N1B4_1785 at 2037.835 kb on - strand, within Pf1N1B4_1785 at 2037.944 kb on + strand at 2037.944 kb on + strand at 2037.944 kb on + strand at 2037.945 kb on - strand at 2037.945 kb on - strand at 2037.945 kb on - strand at 2037.945 kb on - strand at 2037.945 kb on - strand at 2037.945 kb on - strand at 2037.945 kb on - strand at 2038.067 kb on + strand at 2038.067 kb on + strand at 2038.067 kb on + strand at 2038.067 kb on + strand at 2038.067 kb on + strand at 2038.068 kb on - strand at 2038.068 kb on - strand at 2038.068 kb on - strand at 2038.068 kb on - strand at 2038.088 kb on + strand at 2038.089 kb on - strand at 2038.089 kb on - strand at 2038.089 kb on - strand at 2038.089 kb on - strand at 2038.089 kb on - strand at 2038.089 kb on - strand at 2038.243 kb on + strand at 2038.243 kb on + strand at 2038.243 kb on + strand at 2038.243 kb on + strand at 2038.243 kb on + strand at 2038.244 kb on - strand at 2038.244 kb on - strand at 2038.244 kb on - strand at 2038.244 kb on - strand at 2038.244 kb on - strand at 2038.244 kb on - strand at 2038.244 kb on - strand at 2038.244 kb on - strand at 2038.253 kb on + strand at 2038.300 kb on + strand at 2038.300 kb on + strand at 2038.339 kb on + strand at 2038.339 kb on + strand at 2038.339 kb on + strand at 2038.340 kb on - strand at 2038.340 kb on - strand at 2038.340 kb on - strand at 2038.340 kb on - strand at 2038.548 kb on + strand, within Pf1N1B4_1786 at 2038.753 kb on + strand, within Pf1N1B4_1786 at 2038.753 kb on + strand, within Pf1N1B4_1786 at 2038.754 kb on - strand, within Pf1N1B4_1786 at 2038.754 kb on - strand, within Pf1N1B4_1786 at 2038.788 kb on - strand, within Pf1N1B4_1786 at 2038.885 kb on + strand, within Pf1N1B4_1786 at 2038.885 kb on + strand, within Pf1N1B4_1786 at 2039.026 kb on + strand, within Pf1N1B4_1786 at 2039.033 kb on + strand, within Pf1N1B4_1786 at 2039.033 kb on + strand, within Pf1N1B4_1786 at 2039.034 kb on - strand, within Pf1N1B4_1786 at 2039.034 kb on - strand, within Pf1N1B4_1786 at 2039.034 kb on - strand, within Pf1N1B4_1786 at 2039.034 kb on - strand, within Pf1N1B4_1786 at 2039.212 kb on + strand, within Pf1N1B4_1786 at 2039.252 kb on - strand, within Pf1N1B4_1786 at 2039.268 kb on - strand, within Pf1N1B4_1786 at 2039.366 kb on + strand, within Pf1N1B4_1786 at 2039.546 kb on + strand, within Pf1N1B4_1786 at 2039.552 kb on + strand, within Pf1N1B4_1786 at 2039.552 kb on + strand, within Pf1N1B4_1786 at 2039.553 kb on - strand, within Pf1N1B4_1786 at 2039.553 kb on - strand, within Pf1N1B4_1786 at 2039.553 kb on - strand, within Pf1N1B4_1786 at 2039.576 kb on + strand, within Pf1N1B4_1786 at 2039.576 kb on + strand, within Pf1N1B4_1786 at 2039.576 kb on + strand, within Pf1N1B4_1786 at 2039.576 kb on + strand, within Pf1N1B4_1786 at 2039.576 kb on + strand, within Pf1N1B4_1786 at 2039.577 kb on - strand at 2039.577 kb on - strand, within Pf1N1B4_1786 at 2039.577 kb on - strand, within Pf1N1B4_1786 at 2039.577 kb on - strand, within Pf1N1B4_1786 at 2039.699 kb on + strand, within Pf1N1B4_1786 at 2039.699 kb on + strand, within Pf1N1B4_1786 at 2039.700 kb on - strand, within Pf1N1B4_1786 at 2039.708 kb on + strand, within Pf1N1B4_1786 at 2039.709 kb on - strand, within Pf1N1B4_1786 at 2039.747 kb on + strand, within Pf1N1B4_1786 at 2039.748 kb on - strand, within Pf1N1B4_1786 at 2039.748 kb on - strand, within Pf1N1B4_1786 at 2039.802 kb on - strand, within Pf1N1B4_1786 at 2039.802 kb on - strand, within Pf1N1B4_1786 at 2039.802 kb on - strand, within Pf1N1B4_1786 at 2039.858 kb on + strand, within Pf1N1B4_1786 at 2039.859 kb on - strand, within Pf1N1B4_1786 at 2039.954 kb on + strand, within Pf1N1B4_1786 at 2039.955 kb on - strand, within Pf1N1B4_1786 at 2039.955 kb on - strand, within Pf1N1B4_1786 at 2039.955 kb on - strand, within Pf1N1B4_1786 at 2040.075 kb on + strand, within Pf1N1B4_1786 at 2040.075 kb on + strand at 2040.075 kb on + strand, within Pf1N1B4_1786 at 2040.075 kb on + strand, within Pf1N1B4_1786 at 2040.075 kb on + strand, within Pf1N1B4_1786 at 2040.075 kb on + strand, within Pf1N1B4_1786 at 2040.076 kb on - strand, within Pf1N1B4_1786 at 2040.076 kb on - strand, within Pf1N1B4_1786 at 2040.076 kb on - strand, within Pf1N1B4_1786 at 2040.175 kb on + strand at 2040.176 kb on - strand at 2040.225 kb on - strand at 2040.401 kb on - strand at 2040.401 kb on - strand at 2040.462 kb on - strand at 2040.462 kb on - strand at 2040.488 kb on - strand at 2040.665 kb on + strand, within Pf1N1B4_1787 at 2040.665 kb on + strand, within Pf1N1B4_1787 at 2040.666 kb on - strand, within Pf1N1B4_1787 at 2040.666 kb on - strand, within Pf1N1B4_1787 at 2040.666 kb on - strand, within Pf1N1B4_1787 at 2040.666 kb on - strand, within Pf1N1B4_1787 at 2040.868 kb on + strand, within Pf1N1B4_1787 at 2040.868 kb on + strand, within Pf1N1B4_1787 at 2040.868 kb on + strand, within Pf1N1B4_1787 at 2040.868 kb on + strand, within Pf1N1B4_1787 at 2040.868 kb on + strand, within Pf1N1B4_1787 at 2040.869 kb on - strand, within Pf1N1B4_1787 at 2040.869 kb on - strand, within Pf1N1B4_1787 at 2041.021 kb on - strand, within Pf1N1B4_1787 at 2041.103 kb on + strand, within Pf1N1B4_1787 at 2041.103 kb on + strand, within Pf1N1B4_1787 at 2041.103 kb on + strand, within Pf1N1B4_1787 at 2041.104 kb on - strand, within Pf1N1B4_1787 at 2041.155 kb on + strand, within Pf1N1B4_1787 at 2041.156 kb on - strand, within Pf1N1B4_1787 at 2041.156 kb on - strand, within Pf1N1B4_1787 at 2041.156 kb on - strand, within Pf1N1B4_1787 at 2041.156 kb on - strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.206 kb on - strand, within Pf1N1B4_1787 at 2041.206 kb on - strand, within Pf1N1B4_1787
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 2,037,377 + +0.4 2,037,418 + -1.2 2,037,418 + +2.4 2,037,418 + +1.1 2,037,418 + -0.0 2,037,418 + +0.4 2,037,418 + -1.9 2,037,418 + +0.8 2,037,419 - -0.6 2,037,419 - +0.4 2,037,419 - +0.4 2,037,419 - +1.5 2,037,423 - +0.4 2,037,423 - +0.1 2,037,433 + -0.2 2,037,434 - -1.1 2,037,437 + +0.1 2,037,437 + -1.7 2,037,437 + +2.0 2,037,437 + -0.3 2,037,438 - -0.1 2,037,438 - -1.2 2,037,438 - +0.2 2,037,593 - Pf1N1B4_1785 0.34 +0.2 2,037,723 + Pf1N1B4_1785 0.61 -0.5 2,037,723 + Pf1N1B4_1785 0.61 -0.1 2,037,723 + Pf1N1B4_1785 0.61 +1.4 2,037,723 + Pf1N1B4_1785 0.61 -0.1 2,037,723 + Pf1N1B4_1785 0.61 +1.0 2,037,723 + Pf1N1B4_1785 0.61 +0.7 2,037,835 - Pf1N1B4_1785 0.83 -0.4 2,037,944 + +0.4 2,037,944 + +1.3 2,037,944 + +1.4 2,037,945 - +0.8 2,037,945 - +0.4 2,037,945 - +0.4 2,037,945 - +2.7 2,037,945 - -0.6 2,037,945 - +0.1 2,037,945 - -0.1 2,038,067 + +0.8 2,038,067 + -1.2 2,038,067 + -0.4 2,038,067 + +0.7 2,038,067 + -2.2 2,038,068 - +1.0 2,038,068 - -1.3 2,038,068 - +1.7 2,038,068 - -1.2 2,038,088 + +0.7 2,038,089 - +0.2 2,038,089 - +0.4 2,038,089 - +0.4 2,038,089 - +0.1 2,038,089 - +2.1 2,038,089 - +1.7 2,038,243 + +1.0 2,038,243 + -0.6 2,038,243 + -1.3 2,038,243 + -0.3 2,038,243 + +0.4 2,038,244 - +1.1 2,038,244 - -1.9 2,038,244 - +0.7 2,038,244 - +2.2 2,038,244 - -0.6 2,038,244 - +0.3 2,038,244 - +0.1 2,038,244 - -1.2 2,038,253 + +0.4 2,038,300 + +0.4 2,038,300 + +1.1 2,038,339 + -1.2 2,038,339 + -0.9 2,038,339 + -0.2 2,038,340 - -0.9 2,038,340 - -1.0 2,038,340 - +0.5 2,038,340 - -0.3 2,038,548 + Pf1N1B4_1786 0.11 -0.8 2,038,753 + Pf1N1B4_1786 0.21 +0.4 2,038,753 + Pf1N1B4_1786 0.21 -1.6 2,038,754 - Pf1N1B4_1786 0.21 +2.0 2,038,754 - Pf1N1B4_1786 0.21 +0.4 2,038,788 - Pf1N1B4_1786 0.23 +0.2 2,038,885 + Pf1N1B4_1786 0.28 +0.3 2,038,885 + Pf1N1B4_1786 0.28 -0.3 2,039,026 + Pf1N1B4_1786 0.35 +0.3 2,039,033 + Pf1N1B4_1786 0.36 -1.6 2,039,033 + Pf1N1B4_1786 0.36 +2.6 2,039,034 - Pf1N1B4_1786 0.36 -0.8 2,039,034 - Pf1N1B4_1786 0.36 -1.5 2,039,034 - Pf1N1B4_1786 0.36 +1.4 2,039,034 - Pf1N1B4_1786 0.36 +0.2 2,039,212 + Pf1N1B4_1786 0.45 +1.1 2,039,252 - Pf1N1B4_1786 0.47 -0.6 2,039,268 - Pf1N1B4_1786 0.48 +0.8 2,039,366 + Pf1N1B4_1786 0.53 +0.4 2,039,546 + Pf1N1B4_1786 0.62 +0.4 2,039,552 + Pf1N1B4_1786 0.63 -1.4 2,039,552 + Pf1N1B4_1786 0.63 +1.7 2,039,553 - Pf1N1B4_1786 0.63 -2.4 2,039,553 - Pf1N1B4_1786 0.63 +0.4 2,039,553 - Pf1N1B4_1786 0.63 +0.0 2,039,576 + Pf1N1B4_1786 0.64 +1.4 2,039,576 + Pf1N1B4_1786 0.64 -1.1 2,039,576 + Pf1N1B4_1786 0.64 -0.5 2,039,576 + Pf1N1B4_1786 0.64 -0.9 2,039,576 + Pf1N1B4_1786 0.64 -0.4 2,039,577 - +0.4 2,039,577 - Pf1N1B4_1786 0.64 +1.1 2,039,577 - Pf1N1B4_1786 0.64 -0.1 2,039,577 - Pf1N1B4_1786 0.64 -0.9 2,039,699 + Pf1N1B4_1786 0.70 -1.6 2,039,699 + Pf1N1B4_1786 0.70 -2.4 2,039,700 - Pf1N1B4_1786 0.70 -0.1 2,039,708 + Pf1N1B4_1786 0.71 +0.6 2,039,709 - Pf1N1B4_1786 0.71 -1.6 2,039,747 + Pf1N1B4_1786 0.73 -0.2 2,039,748 - Pf1N1B4_1786 0.73 +1.4 2,039,748 - Pf1N1B4_1786 0.73 +0.8 2,039,802 - Pf1N1B4_1786 0.76 +0.4 2,039,802 - Pf1N1B4_1786 0.76 +0.7 2,039,802 - Pf1N1B4_1786 0.76 +0.8 2,039,858 + Pf1N1B4_1786 0.78 +0.4 2,039,859 - Pf1N1B4_1786 0.79 +2.0 2,039,954 + Pf1N1B4_1786 0.83 -0.6 2,039,955 - Pf1N1B4_1786 0.83 -0.6 2,039,955 - Pf1N1B4_1786 0.83 +0.2 2,039,955 - Pf1N1B4_1786 0.83 -0.3 2,040,075 + Pf1N1B4_1786 0.90 -0.0 2,040,075 + +1.4 2,040,075 + Pf1N1B4_1786 0.90 -2.1 2,040,075 + Pf1N1B4_1786 0.90 -1.9 2,040,075 + Pf1N1B4_1786 0.90 +0.4 2,040,075 + Pf1N1B4_1786 0.90 +0.9 2,040,076 - Pf1N1B4_1786 0.90 +0.4 2,040,076 - Pf1N1B4_1786 0.90 -1.9 2,040,076 - Pf1N1B4_1786 0.90 -0.0 2,040,175 + -1.6 2,040,176 - -0.6 2,040,225 - -0.6 2,040,401 - -0.2 2,040,401 - -1.1 2,040,462 - -0.6 2,040,462 - +0.4 2,040,488 - +0.7 2,040,665 + Pf1N1B4_1787 0.13 +1.7 2,040,665 + Pf1N1B4_1787 0.13 -0.2 2,040,666 - Pf1N1B4_1787 0.13 +0.8 2,040,666 - Pf1N1B4_1787 0.13 -1.2 2,040,666 - Pf1N1B4_1787 0.13 +0.4 2,040,666 - Pf1N1B4_1787 0.13 +0.4 2,040,868 + Pf1N1B4_1787 0.30 -0.9 2,040,868 + Pf1N1B4_1787 0.30 -0.2 2,040,868 + Pf1N1B4_1787 0.30 +0.4 2,040,868 + Pf1N1B4_1787 0.30 +1.0 2,040,868 + Pf1N1B4_1787 0.30 -1.4 2,040,869 - Pf1N1B4_1787 0.30 +0.4 2,040,869 - Pf1N1B4_1787 0.30 -0.6 2,041,021 - Pf1N1B4_1787 0.42 +1.4 2,041,103 + Pf1N1B4_1787 0.49 +1.1 2,041,103 + Pf1N1B4_1787 0.49 +0.8 2,041,103 + Pf1N1B4_1787 0.49 -0.9 2,041,104 - Pf1N1B4_1787 0.49 -0.7 2,041,155 + Pf1N1B4_1787 0.53 +2.7 2,041,156 - Pf1N1B4_1787 0.53 -0.2 2,041,156 - Pf1N1B4_1787 0.53 +1.1 2,041,156 - Pf1N1B4_1787 0.53 +1.1 2,041,156 - Pf1N1B4_1787 0.53 -1.6 2,041,205 + Pf1N1B4_1787 0.57 -0.6 2,041,205 + Pf1N1B4_1787 0.57 +0.4 2,041,205 + Pf1N1B4_1787 0.57 +0.7 2,041,205 + Pf1N1B4_1787 0.57 -0.3 2,041,205 + Pf1N1B4_1787 0.57 -0.3 2,041,205 + Pf1N1B4_1787 0.57 +3.2 2,041,206 - Pf1N1B4_1787 0.57 -0.4 2,041,206 - Pf1N1B4_1787 0.57 +2.2
Or see this region's nucleotide sequence