Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1707 and Pf1N1B4_1708 overlap by 4 nucleotides Pf1N1B4_1708 and Pf1N1B4_1709 are separated by 213 nucleotides
Pf1N1B4_1707 - Efflux membrane fusion protein, RND family, at 1,954,717 to 1,955,784
_1707
Pf1N1B4_1708 - Efflux transporter, RND family, MFP subunit, at 1,955,781 to 1,956,884
_1708
Pf1N1B4_1709 - putative RecF protein, at 1,957,098 to 1,958,267
_1709
Position (kb)
1955
1956
1957 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1955.237 kb on - strand at 1955.291 kb on - strand at 1955.291 kb on - strand, within Pf1N1B4_1707 at 1955.300 kb on + strand, within Pf1N1B4_1707 at 1955.300 kb on + strand, within Pf1N1B4_1707 at 1955.300 kb on + strand, within Pf1N1B4_1707 at 1955.301 kb on - strand, within Pf1N1B4_1707 at 1955.301 kb on - strand, within Pf1N1B4_1707 at 1955.301 kb on - strand, within Pf1N1B4_1707 at 1955.301 kb on - strand, within Pf1N1B4_1707 at 1955.812 kb on + strand at 1955.813 kb on - strand at 1955.813 kb on - strand at 1955.813 kb on - strand at 1955.813 kb on - strand at 1955.853 kb on + strand at 1955.853 kb on + strand at 1955.853 kb on + strand at 1955.854 kb on - strand at 1956.221 kb on + strand, within Pf1N1B4_1708 at 1956.254 kb on - strand, within Pf1N1B4_1708 at 1956.254 kb on - strand, within Pf1N1B4_1708 at 1956.318 kb on + strand, within Pf1N1B4_1708 at 1956.318 kb on + strand, within Pf1N1B4_1708 at 1956.319 kb on - strand, within Pf1N1B4_1708 at 1956.319 kb on - strand, within Pf1N1B4_1708 at 1956.319 kb on - strand, within Pf1N1B4_1708 at 1956.319 kb on - strand, within Pf1N1B4_1708 at 1956.385 kb on + strand, within Pf1N1B4_1708 at 1956.386 kb on - strand, within Pf1N1B4_1708 at 1956.475 kb on + strand, within Pf1N1B4_1708 at 1956.476 kb on - strand, within Pf1N1B4_1708 at 1956.493 kb on + strand, within Pf1N1B4_1708 at 1956.903 kb on + strand at 1956.903 kb on + strand at 1956.903 kb on + strand at 1956.903 kb on + strand at 1956.903 kb on + strand at 1956.903 kb on + strand at 1956.904 kb on - strand at 1956.923 kb on + strand at 1956.923 kb on + strand at 1956.923 kb on + strand at 1956.937 kb on + strand at 1956.937 kb on + strand at 1956.937 kb on + strand at 1956.937 kb on + strand at 1956.938 kb on - strand at 1956.938 kb on - strand at 1956.971 kb on + strand at 1956.971 kb on + strand at 1956.971 kb on + strand at 1956.972 kb on - strand at 1956.972 kb on - strand at 1956.973 kb on + strand at 1956.973 kb on + strand at 1956.973 kb on + strand at 1956.973 kb on + strand at 1956.973 kb on + strand at 1956.973 kb on + strand at 1956.974 kb on - strand at 1956.974 kb on - strand at 1956.974 kb on - strand at 1956.974 kb on - strand at 1956.974 kb on - strand at 1957.139 kb on + strand at 1957.139 kb on + strand at 1957.140 kb on - strand at 1957.326 kb on - strand, within Pf1N1B4_1709 at 1957.326 kb on - strand, within Pf1N1B4_1709 at 1957.413 kb on + strand, within Pf1N1B4_1709 at 1957.414 kb on - strand at 1957.414 kb on - strand, within Pf1N1B4_1709 at 1957.461 kb on + strand, within Pf1N1B4_1709 at 1957.461 kb on + strand, within Pf1N1B4_1709 at 1957.462 kb on - strand, within Pf1N1B4_1709 at 1957.588 kb on + strand, within Pf1N1B4_1709 at 1957.588 kb on + strand, within Pf1N1B4_1709 at 1957.699 kb on - strand, within Pf1N1B4_1709 at 1957.881 kb on - strand, within Pf1N1B4_1709
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 1,955,237 - -0.6 1,955,291 - +0.4 1,955,291 - Pf1N1B4_1707 0.54 +2.7 1,955,300 + Pf1N1B4_1707 0.55 -1.6 1,955,300 + Pf1N1B4_1707 0.55 +0.9 1,955,300 + Pf1N1B4_1707 0.55 +0.6 1,955,301 - Pf1N1B4_1707 0.55 -1.8 1,955,301 - Pf1N1B4_1707 0.55 +1.6 1,955,301 - Pf1N1B4_1707 0.55 +1.1 1,955,301 - Pf1N1B4_1707 0.55 -1.6 1,955,812 + +0.1 1,955,813 - -0.4 1,955,813 - +0.4 1,955,813 - -0.0 1,955,813 - -0.2 1,955,853 + +0.6 1,955,853 + +1.6 1,955,853 + +0.4 1,955,854 - -0.8 1,956,221 + Pf1N1B4_1708 0.40 -2.9 1,956,254 - Pf1N1B4_1708 0.43 +1.0 1,956,254 - Pf1N1B4_1708 0.43 -1.4 1,956,318 + Pf1N1B4_1708 0.49 +0.2 1,956,318 + Pf1N1B4_1708 0.49 -1.0 1,956,319 - Pf1N1B4_1708 0.49 +0.2 1,956,319 - Pf1N1B4_1708 0.49 +1.4 1,956,319 - Pf1N1B4_1708 0.49 -1.0 1,956,319 - Pf1N1B4_1708 0.49 -2.2 1,956,385 + Pf1N1B4_1708 0.55 +1.0 1,956,386 - Pf1N1B4_1708 0.55 -0.6 1,956,475 + Pf1N1B4_1708 0.63 -1.8 1,956,476 - Pf1N1B4_1708 0.63 -1.9 1,956,493 + Pf1N1B4_1708 0.64 -1.9 1,956,903 + -0.6 1,956,903 + +0.4 1,956,903 + +0.8 1,956,903 + -0.2 1,956,903 + +2.7 1,956,903 + +2.0 1,956,904 - -0.4 1,956,923 + +0.4 1,956,923 + +0.8 1,956,923 + -0.1 1,956,937 + +0.4 1,956,937 + -1.2 1,956,937 + -0.6 1,956,937 + +1.4 1,956,938 - -0.0 1,956,938 - +0.7 1,956,971 + -0.3 1,956,971 + +2.1 1,956,971 + +0.4 1,956,972 - -1.2 1,956,972 - +0.4 1,956,973 + +2.4 1,956,973 + +0.2 1,956,973 + -1.9 1,956,973 + +0.6 1,956,973 + -0.9 1,956,973 + +1.0 1,956,974 - +0.6 1,956,974 - +0.7 1,956,974 - +1.7 1,956,974 - -1.2 1,956,974 - -0.7 1,957,139 + -2.4 1,957,139 + +0.7 1,957,140 - +0.1 1,957,326 - Pf1N1B4_1709 0.19 +1.0 1,957,326 - Pf1N1B4_1709 0.19 -0.7 1,957,413 + Pf1N1B4_1709 0.27 +0.2 1,957,414 - -0.6 1,957,414 - Pf1N1B4_1709 0.27 -1.1 1,957,461 + Pf1N1B4_1709 0.31 -0.1 1,957,461 + Pf1N1B4_1709 0.31 -0.6 1,957,462 - Pf1N1B4_1709 0.31 +1.4 1,957,588 + Pf1N1B4_1709 0.42 -0.5 1,957,588 + Pf1N1B4_1709 0.42 +1.1 1,957,699 - Pf1N1B4_1709 0.51 -0.6 1,957,881 - Pf1N1B4_1709 0.67 -0.8
Or see this region's nucleotide sequence