Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1546 and Pf1N1B4_1547 are separated by 13 nucleotides Pf1N1B4_1547 and Pf1N1B4_1548 are separated by 302 nucleotides Pf1N1B4_1548 and Pf1N1B4_1549 overlap by 4 nucleotides Pf1N1B4_1549 and Pf1N1B4_1550 are separated by 238 nucleotides
Pf1N1B4_1546 - Thermonuclease family protein, at 1,784,111 to 1,784,911
_1546
Pf1N1B4_1547 - LSU ribosomal protein L31p @ LSU ribosomal protein L31p, zinc-dependent, at 1,784,925 to 1,785,155
_1547
Pf1N1B4_1548 - Helicase PriA essential for oriC/DnaA-independent DNA replication, at 1,785,458 to 1,787,251
_1548
Pf1N1B4_1549 - Helicase PriA essential for oriC/DnaA-independent DNA replication, at 1,787,248 to 1,787,676
_1549
Pf1N1B4_1550 - Arginyl-tRNA synthetase (EC 6.1.1.19), at 1,787,915 to 1,789,651
_1550
Position (kb)
1785
1786
1787
1788 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.719 kb on + strand, within Pf1N1B4_1546 at 1785.217 kb on - strand at 1785.217 kb on - strand at 1785.217 kb on - strand at 1785.217 kb on - strand at 1785.263 kb on + strand at 1785.375 kb on + strand at 1785.376 kb on - strand at 1785.376 kb on - strand at 1785.430 kb on + strand at 1785.430 kb on + strand at 1787.671 kb on + strand at 1787.671 kb on + strand at 1787.671 kb on + strand at 1787.739 kb on + strand at 1787.739 kb on + strand at 1787.739 kb on + strand at 1787.739 kb on + strand at 1787.739 kb on + strand at 1787.739 kb on + strand at 1787.739 kb on + strand at 1787.739 kb on + strand at 1787.739 kb on + strand at 1787.739 kb on + strand at 1787.739 kb on + strand at 1787.739 kb on + strand at 1787.746 kb on + strand at 1787.746 kb on + strand at 1787.746 kb on + strand at 1787.746 kb on + strand at 1787.753 kb on + strand at 1787.753 kb on + strand at 1787.753 kb on + strand at 1787.753 kb on + strand at 1787.754 kb on - strand at 1787.754 kb on - strand at 1787.803 kb on + strand at 1787.803 kb on + strand at 1787.803 kb on + strand at 1787.803 kb on + strand at 1787.803 kb on + strand at 1787.821 kb on + strand at 1787.823 kb on + strand at 1787.823 kb on + strand at 1787.823 kb on + strand at 1787.823 kb on + strand at 1787.823 kb on + strand at 1787.823 kb on + strand at 1787.823 kb on + strand at 1787.823 kb on + strand at 1787.823 kb on + strand at 1787.823 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 1,784,562 + Pf1N1B4_1546 0.56 -1.3 1,784,562 + Pf1N1B4_1546 0.56 -0.0 1,784,562 + Pf1N1B4_1546 0.56 +0.8 1,784,562 + Pf1N1B4_1546 0.56 +0.2 1,784,562 + Pf1N1B4_1546 0.56 +2.3 1,784,562 + Pf1N1B4_1546 0.56 +1.1 1,784,562 + Pf1N1B4_1546 0.56 -0.3 1,784,563 - Pf1N1B4_1546 0.56 +0.3 1,784,563 - Pf1N1B4_1546 0.56 +0.4 1,784,563 - Pf1N1B4_1546 0.56 +0.6 1,784,563 - Pf1N1B4_1546 0.56 -1.3 1,784,563 - Pf1N1B4_1546 0.56 -0.7 1,784,563 - Pf1N1B4_1546 0.56 +0.3 1,784,719 + Pf1N1B4_1546 0.76 -0.7 1,785,217 - +1.9 1,785,217 - -0.3 1,785,217 - +1.6 1,785,217 - -2.0 1,785,263 + -0.9 1,785,375 + -1.3 1,785,376 - -0.0 1,785,376 - -0.6 1,785,430 + +0.3 1,785,430 + +0.9 1,787,671 + +0.3 1,787,671 + +1.2 1,787,671 + +1.8 1,787,739 + -0.6 1,787,739 + +0.5 1,787,739 + +0.3 1,787,739 + +1.0 1,787,739 + +1.1 1,787,739 + +0.7 1,787,739 + -0.9 1,787,739 + +0.9 1,787,739 + +0.8 1,787,739 + -0.1 1,787,739 + +0.3 1,787,739 + +1.0 1,787,746 + +1.3 1,787,746 + +2.2 1,787,746 + +0.0 1,787,746 + +0.8 1,787,753 + +0.8 1,787,753 + +1.3 1,787,753 + +0.8 1,787,753 + -0.2 1,787,754 - -1.0 1,787,754 - -1.9 1,787,803 + +0.3 1,787,803 + +1.4 1,787,803 + +1.3 1,787,803 + +2.3 1,787,803 + +0.3 1,787,821 + +0.3 1,787,823 + +0.5 1,787,823 + +1.4 1,787,823 + +2.6 1,787,823 + -1.1 1,787,823 + +1.3 1,787,823 + +1.1 1,787,823 + -1.7 1,787,823 + +0.3 1,787,823 + +0.8 1,787,823 + +0.3
Or see this region's nucleotide sequence