Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1548

Experiment: Cisplatin 0.006 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1546 and Pf1N1B4_1547 are separated by 13 nucleotidesPf1N1B4_1547 and Pf1N1B4_1548 are separated by 302 nucleotidesPf1N1B4_1548 and Pf1N1B4_1549 overlap by 4 nucleotidesPf1N1B4_1549 and Pf1N1B4_1550 are separated by 238 nucleotides Pf1N1B4_1546 - Thermonuclease family protein, at 1,784,111 to 1,784,911 _1546 Pf1N1B4_1547 - LSU ribosomal protein L31p @ LSU ribosomal protein L31p, zinc-dependent, at 1,784,925 to 1,785,155 _1547 Pf1N1B4_1548 - Helicase PriA essential for oriC/DnaA-independent DNA replication, at 1,785,458 to 1,787,251 _1548 Pf1N1B4_1549 - Helicase PriA essential for oriC/DnaA-independent DNA replication, at 1,787,248 to 1,787,676 _1549 Pf1N1B4_1550 - Arginyl-tRNA synthetase (EC 6.1.1.19), at 1,787,915 to 1,789,651 _1550 Position (kb) 1785 1786 1787 1788Strain fitness (log2 ratio) -2 -1 0 1 2at 1784.562 kb on + strand, within Pf1N1B4_1546at 1784.562 kb on + strand, within Pf1N1B4_1546at 1784.562 kb on + strand, within Pf1N1B4_1546at 1784.562 kb on + strand, within Pf1N1B4_1546at 1784.562 kb on + strand, within Pf1N1B4_1546at 1784.562 kb on + strand, within Pf1N1B4_1546at 1784.562 kb on + strand, within Pf1N1B4_1546at 1784.563 kb on - strand, within Pf1N1B4_1546at 1784.563 kb on - strand, within Pf1N1B4_1546at 1784.563 kb on - strand, within Pf1N1B4_1546at 1784.563 kb on - strand, within Pf1N1B4_1546at 1784.563 kb on - strand, within Pf1N1B4_1546at 1784.563 kb on - strand, within Pf1N1B4_1546at 1784.719 kb on + strand, within Pf1N1B4_1546at 1785.217 kb on - strandat 1785.217 kb on - strandat 1785.217 kb on - strandat 1785.217 kb on - strandat 1785.263 kb on + strandat 1785.375 kb on + strandat 1785.376 kb on - strandat 1785.376 kb on - strandat 1785.430 kb on + strandat 1785.430 kb on + strandat 1787.671 kb on + strandat 1787.671 kb on + strandat 1787.671 kb on + strandat 1787.739 kb on + strandat 1787.739 kb on + strandat 1787.739 kb on + strandat 1787.739 kb on + strandat 1787.739 kb on + strandat 1787.739 kb on + strandat 1787.739 kb on + strandat 1787.739 kb on + strandat 1787.739 kb on + strandat 1787.739 kb on + strandat 1787.739 kb on + strandat 1787.739 kb on + strandat 1787.746 kb on + strandat 1787.746 kb on + strandat 1787.746 kb on + strandat 1787.746 kb on + strandat 1787.753 kb on + strandat 1787.753 kb on + strandat 1787.753 kb on + strandat 1787.753 kb on + strandat 1787.754 kb on - strandat 1787.754 kb on - strandat 1787.803 kb on + strandat 1787.803 kb on + strandat 1787.803 kb on + strandat 1787.803 kb on + strandat 1787.803 kb on + strandat 1787.821 kb on + strandat 1787.823 kb on + strandat 1787.823 kb on + strandat 1787.823 kb on + strandat 1787.823 kb on + strandat 1787.823 kb on + strandat 1787.823 kb on + strandat 1787.823 kb on + strandat 1787.823 kb on + strandat 1787.823 kb on + strandat 1787.823 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml
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1,784,562 + Pf1N1B4_1546 0.56 -1.3
1,784,562 + Pf1N1B4_1546 0.56 -0.0
1,784,562 + Pf1N1B4_1546 0.56 +0.8
1,784,562 + Pf1N1B4_1546 0.56 +0.2
1,784,562 + Pf1N1B4_1546 0.56 +2.3
1,784,562 + Pf1N1B4_1546 0.56 +1.1
1,784,562 + Pf1N1B4_1546 0.56 -0.3
1,784,563 - Pf1N1B4_1546 0.56 +0.3
1,784,563 - Pf1N1B4_1546 0.56 +0.4
1,784,563 - Pf1N1B4_1546 0.56 +0.6
1,784,563 - Pf1N1B4_1546 0.56 -1.3
1,784,563 - Pf1N1B4_1546 0.56 -0.7
1,784,563 - Pf1N1B4_1546 0.56 +0.3
1,784,719 + Pf1N1B4_1546 0.76 -0.7
1,785,217 - +1.9
1,785,217 - -0.3
1,785,217 - +1.6
1,785,217 - -2.0
1,785,263 + -0.9
1,785,375 + -1.3
1,785,376 - -0.0
1,785,376 - -0.6
1,785,430 + +0.3
1,785,430 + +0.9
1,787,671 + +0.3
1,787,671 + +1.2
1,787,671 + +1.8
1,787,739 + -0.6
1,787,739 + +0.5
1,787,739 + +0.3
1,787,739 + +1.0
1,787,739 + +1.1
1,787,739 + +0.7
1,787,739 + -0.9
1,787,739 + +0.9
1,787,739 + +0.8
1,787,739 + -0.1
1,787,739 + +0.3
1,787,739 + +1.0
1,787,746 + +1.3
1,787,746 + +2.2
1,787,746 + +0.0
1,787,746 + +0.8
1,787,753 + +0.8
1,787,753 + +1.3
1,787,753 + +0.8
1,787,753 + -0.2
1,787,754 - -1.0
1,787,754 - -1.9
1,787,803 + +0.3
1,787,803 + +1.4
1,787,803 + +1.3
1,787,803 + +2.3
1,787,803 + +0.3
1,787,821 + +0.3
1,787,823 + +0.5
1,787,823 + +1.4
1,787,823 + +2.6
1,787,823 + -1.1
1,787,823 + +1.3
1,787,823 + +1.1
1,787,823 + -1.7
1,787,823 + +0.3
1,787,823 + +0.8
1,787,823 + +0.3

Or see this region's nucleotide sequence