Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_138 and Pf1N1B4_139 overlap by 4 nucleotides Pf1N1B4_139 and Pf1N1B4_140 are separated by 43 nucleotides
Pf1N1B4_138 - Putative dipeptidase, pyoverdin biosynthesis PvdM, at 154,539 to 155,915
_138
Pf1N1B4_139 - Pyoverdin biosynthesis protein PvdN, putative aminotransferase, class V, at 155,912 to 157,198
_139
Pf1N1B4_140 - PvdO, pyoverdine responsive serine/threonine kinase (predicted by OlgaV), at 157,242 to 158,129
_140
Position (kb) 155
156
157
158 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 155.611 kb on - strand at 155.670 kb on + strand, within Pf1N1B4_138 at 155.671 kb on - strand, within Pf1N1B4_138 at 156.071 kb on + strand, within Pf1N1B4_139 at 156.072 kb on - strand, within Pf1N1B4_139 at 156.072 kb on - strand, within Pf1N1B4_139 at 156.360 kb on - strand, within Pf1N1B4_139 at 156.360 kb on - strand, within Pf1N1B4_139 at 156.360 kb on - strand, within Pf1N1B4_139 at 156.617 kb on + strand, within Pf1N1B4_139 at 156.618 kb on - strand, within Pf1N1B4_139 at 156.618 kb on - strand, within Pf1N1B4_139 at 157.242 kb on - strand at 157.242 kb on - strand at 157.542 kb on + strand, within Pf1N1B4_140 at 157.677 kb on + strand, within Pf1N1B4_140 at 157.677 kb on + strand, within Pf1N1B4_140 at 157.677 kb on + strand, within Pf1N1B4_140 at 157.677 kb on + strand, within Pf1N1B4_140 at 157.743 kb on + strand, within Pf1N1B4_140 at 157.744 kb on - strand at 157.831 kb on - strand, within Pf1N1B4_140 at 157.848 kb on + strand, within Pf1N1B4_140 at 157.921 kb on - strand, within Pf1N1B4_140 at 157.921 kb on - strand, within Pf1N1B4_140 at 157.953 kb on + strand, within Pf1N1B4_140 at 157.953 kb on + strand, within Pf1N1B4_140 at 157.954 kb on - strand, within Pf1N1B4_140 at 157.954 kb on - strand, within Pf1N1B4_140 at 157.954 kb on - strand, within Pf1N1B4_140 at 157.954 kb on - strand, within Pf1N1B4_140 at 158.113 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 155,611 - +1.1 155,670 + Pf1N1B4_138 0.82 +1.2 155,671 - Pf1N1B4_138 0.82 +0.5 156,071 + Pf1N1B4_139 0.12 +1.4 156,072 - Pf1N1B4_139 0.12 +1.1 156,072 - Pf1N1B4_139 0.12 -0.8 156,360 - Pf1N1B4_139 0.35 -0.2 156,360 - Pf1N1B4_139 0.35 +0.5 156,360 - Pf1N1B4_139 0.35 +0.8 156,617 + Pf1N1B4_139 0.55 +1.4 156,618 - Pf1N1B4_139 0.55 +1.4 156,618 - Pf1N1B4_139 0.55 +0.8 157,242 - -1.4 157,242 - +1.1 157,542 + Pf1N1B4_140 0.34 +0.4 157,677 + Pf1N1B4_140 0.49 -1.2 157,677 + Pf1N1B4_140 0.49 -0.7 157,677 + Pf1N1B4_140 0.49 -1.8 157,677 + Pf1N1B4_140 0.49 +0.5 157,743 + Pf1N1B4_140 0.56 -1.0 157,744 - -1.2 157,831 - Pf1N1B4_140 0.66 +0.1 157,848 + Pf1N1B4_140 0.68 -1.0 157,921 - Pf1N1B4_140 0.76 -2.5 157,921 - Pf1N1B4_140 0.76 -1.2 157,953 + Pf1N1B4_140 0.80 -3.2 157,953 + Pf1N1B4_140 0.80 +2.4 157,954 - Pf1N1B4_140 0.80 +0.8 157,954 - Pf1N1B4_140 0.80 -0.8 157,954 - Pf1N1B4_140 0.80 +0.8 157,954 - Pf1N1B4_140 0.80 +0.1 158,113 + -0.7
Or see this region's nucleotide sequence