Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1310 and Pf1N1B4_1311 are separated by 30 nucleotides Pf1N1B4_1311 and Pf1N1B4_1312 are separated by 83 nucleotides Pf1N1B4_1312 and Pf1N1B4_1313 overlap by 36 nucleotides
Pf1N1B4_1310 - Ribosomal protein L11 methyltransferase (EC 2.1.1.-), at 1,493,712 to 1,494,590
_1310
Pf1N1B4_1311 - Probable transmembrane protein, at 1,494,621 to 1,495,841
_1311
Pf1N1B4_1312 - hypothetical protein, at 1,495,925 to 1,496,062
_1312
Pf1N1B4_1313 - tRNA dihydrouridine synthase B (EC 1.-.-.-), at 1,496,027 to 1,497,037
_1313
Position (kb)
1494
1495
1496 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1493.651 kb on - strand at 1493.689 kb on + strand at 1493.689 kb on + strand at 1493.689 kb on + strand at 1493.689 kb on + strand at 1493.690 kb on - strand at 1493.691 kb on + strand at 1493.763 kb on + strand at 1493.763 kb on + strand at 1493.763 kb on + strand at 1493.764 kb on - strand at 1494.088 kb on + strand, within Pf1N1B4_1310 at 1494.089 kb on - strand, within Pf1N1B4_1310 at 1494.323 kb on + strand, within Pf1N1B4_1310 at 1494.323 kb on + strand, within Pf1N1B4_1310 at 1494.323 kb on + strand, within Pf1N1B4_1310 at 1494.323 kb on + strand, within Pf1N1B4_1310 at 1494.324 kb on - strand, within Pf1N1B4_1310 at 1494.324 kb on - strand, within Pf1N1B4_1310 at 1494.324 kb on - strand, within Pf1N1B4_1310 at 1494.324 kb on - strand, within Pf1N1B4_1310 at 1494.348 kb on + strand, within Pf1N1B4_1310 at 1494.349 kb on - strand, within Pf1N1B4_1310 at 1494.385 kb on + strand, within Pf1N1B4_1310 at 1494.386 kb on - strand, within Pf1N1B4_1310 at 1494.386 kb on - strand, within Pf1N1B4_1310 at 1494.386 kb on - strand, within Pf1N1B4_1310 at 1494.516 kb on + strand at 1494.573 kb on - strand at 1494.606 kb on - strand at 1494.681 kb on - strand at 1494.734 kb on - strand at 1494.754 kb on + strand, within Pf1N1B4_1311 at 1494.755 kb on - strand, within Pf1N1B4_1311 at 1494.755 kb on - strand, within Pf1N1B4_1311 at 1495.303 kb on + strand, within Pf1N1B4_1311 at 1495.304 kb on - strand, within Pf1N1B4_1311 at 1495.426 kb on - strand, within Pf1N1B4_1311 at 1495.435 kb on - strand, within Pf1N1B4_1311 at 1495.611 kb on + strand, within Pf1N1B4_1311 at 1495.611 kb on + strand, within Pf1N1B4_1311 at 1495.611 kb on + strand, within Pf1N1B4_1311 at 1495.611 kb on + strand, within Pf1N1B4_1311 at 1495.611 kb on + strand, within Pf1N1B4_1311 at 1495.612 kb on - strand, within Pf1N1B4_1311 at 1495.612 kb on - strand, within Pf1N1B4_1311 at 1495.612 kb on - strand, within Pf1N1B4_1311 at 1495.713 kb on + strand, within Pf1N1B4_1311 at 1495.812 kb on + strand at 1495.812 kb on + strand at 1495.812 kb on + strand at 1495.871 kb on + strand at 1495.890 kb on + strand at 1495.893 kb on + strand at 1495.893 kb on + strand at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.051 kb on + strand at 1496.051 kb on + strand at 1496.051 kb on + strand at 1496.051 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.145 kb on + strand, within Pf1N1B4_1313 at 1496.145 kb on + strand, within Pf1N1B4_1313 at 1496.145 kb on + strand, within Pf1N1B4_1313 at 1496.145 kb on + strand, within Pf1N1B4_1313 at 1496.145 kb on + strand, within Pf1N1B4_1313 at 1496.320 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.582 kb on + strand, within Pf1N1B4_1313 at 1496.753 kb on + strand, within Pf1N1B4_1313 at 1496.753 kb on + strand, within Pf1N1B4_1313 at 1496.753 kb on + strand, within Pf1N1B4_1313
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 1,493,651 - +0.6 1,493,689 + -1.2 1,493,689 + +0.1 1,493,689 + +1.0 1,493,689 + +1.8 1,493,690 - -0.6 1,493,691 + -1.6 1,493,763 + -0.6 1,493,763 + -0.6 1,493,763 + -0.0 1,493,764 - +1.8 1,494,088 + Pf1N1B4_1310 0.43 +1.2 1,494,089 - Pf1N1B4_1310 0.43 +1.5 1,494,323 + Pf1N1B4_1310 0.70 +1.5 1,494,323 + Pf1N1B4_1310 0.70 +1.5 1,494,323 + Pf1N1B4_1310 0.70 +0.2 1,494,323 + Pf1N1B4_1310 0.70 -2.4 1,494,324 - Pf1N1B4_1310 0.70 +0.6 1,494,324 - Pf1N1B4_1310 0.70 -0.2 1,494,324 - Pf1N1B4_1310 0.70 +0.6 1,494,324 - Pf1N1B4_1310 0.70 +0.5 1,494,348 + Pf1N1B4_1310 0.72 +1.5 1,494,349 - Pf1N1B4_1310 0.72 -1.6 1,494,385 + Pf1N1B4_1310 0.77 +0.2 1,494,386 - Pf1N1B4_1310 0.77 +1.2 1,494,386 - Pf1N1B4_1310 0.77 +0.2 1,494,386 - Pf1N1B4_1310 0.77 +1.8 1,494,516 + +2.2 1,494,573 - +1.5 1,494,606 - -2.4 1,494,681 - -0.8 1,494,734 - +1.0 1,494,754 + Pf1N1B4_1311 0.11 +0.5 1,494,755 - Pf1N1B4_1311 0.11 +1.1 1,494,755 - Pf1N1B4_1311 0.11 -1.4 1,495,303 + Pf1N1B4_1311 0.56 +0.2 1,495,304 - Pf1N1B4_1311 0.56 +0.6 1,495,426 - Pf1N1B4_1311 0.66 +0.9 1,495,435 - Pf1N1B4_1311 0.67 -1.8 1,495,611 + Pf1N1B4_1311 0.81 +1.1 1,495,611 + Pf1N1B4_1311 0.81 +0.7 1,495,611 + Pf1N1B4_1311 0.81 -1.1 1,495,611 + Pf1N1B4_1311 0.81 +0.8 1,495,611 + Pf1N1B4_1311 0.81 +2.0 1,495,612 - Pf1N1B4_1311 0.81 +0.6 1,495,612 - Pf1N1B4_1311 0.81 +1.4 1,495,612 - Pf1N1B4_1311 0.81 +0.2 1,495,713 + Pf1N1B4_1311 0.89 +1.2 1,495,812 + +0.6 1,495,812 + -0.8 1,495,812 + +1.2 1,495,871 + +0.2 1,495,890 + -2.0 1,495,893 + -0.4 1,495,893 + -2.6 1,496,026 + Pf1N1B4_1312 0.73 -0.2 1,496,026 + Pf1N1B4_1312 0.73 +1.9 1,496,026 + Pf1N1B4_1312 0.73 -0.8 1,496,026 + Pf1N1B4_1312 0.73 +0.9 1,496,026 + Pf1N1B4_1312 0.73 +0.4 1,496,026 + Pf1N1B4_1312 0.73 +0.2 1,496,026 + Pf1N1B4_1312 0.73 +2.2 1,496,026 + Pf1N1B4_1312 0.73 -1.0 1,496,051 + +0.4 1,496,051 + +0.4 1,496,051 + +1.3 1,496,051 + +0.3 1,496,053 + +0.7 1,496,053 + +0.8 1,496,053 + +0.2 1,496,053 + -0.4 1,496,053 + -2.0 1,496,053 + +0.2 1,496,053 + +0.2 1,496,053 + -0.6 1,496,053 + -0.8 1,496,053 + +1.0 1,496,053 + +0.2 1,496,053 + -0.2 1,496,053 + +0.6 1,496,053 + -1.8 1,496,053 + -0.3 1,496,053 + -0.1 1,496,053 + +0.6 1,496,053 + +2.2 1,496,053 + -0.2 1,496,053 + -1.8 1,496,053 + +1.8 1,496,053 + -0.8 1,496,053 + -1.0 1,496,053 + +2.5 1,496,145 + Pf1N1B4_1313 0.12 -0.6 1,496,145 + Pf1N1B4_1313 0.12 +0.2 1,496,145 + Pf1N1B4_1313 0.12 -0.8 1,496,145 + Pf1N1B4_1313 0.12 +0.6 1,496,145 + Pf1N1B4_1313 0.12 -1.8 1,496,320 + Pf1N1B4_1313 0.29 -0.1 1,496,564 + Pf1N1B4_1313 0.53 +1.6 1,496,564 + Pf1N1B4_1313 0.53 +0.9 1,496,564 + Pf1N1B4_1313 0.53 -0.7 1,496,564 + Pf1N1B4_1313 0.53 -1.2 1,496,564 + Pf1N1B4_1313 0.53 -1.6 1,496,564 + Pf1N1B4_1313 0.53 -0.8 1,496,564 + Pf1N1B4_1313 0.53 +1.2 1,496,564 + Pf1N1B4_1313 0.53 -0.8 1,496,582 + Pf1N1B4_1313 0.55 -0.2 1,496,753 + Pf1N1B4_1313 0.72 +0.4 1,496,753 + Pf1N1B4_1313 0.72 -1.4 1,496,753 + Pf1N1B4_1313 0.72 -0.4
Or see this region's nucleotide sequence