Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1245 and Pf1N1B4_1246 are separated by 80 nucleotides Pf1N1B4_1246 and Pf1N1B4_1247 are separated by 82 nucleotides Pf1N1B4_1247 and Pf1N1B4_1248 are separated by 25 nucleotides
Pf1N1B4_1245 - Nucleoside ABC transporter, permease protein 2, at 1,424,043 to 1,424,969
_1245
Pf1N1B4_1246 - FIG00954047: hypothetical protein, at 1,425,050 to 1,425,472
_1246
Pf1N1B4_1247 - transcriptional regulator, MerR family, at 1,425,555 to 1,425,905
_1247
Pf1N1B4_1248 - Probable short-chain dehydrogenase, at 1,425,931 to 1,426,617
_1248
Position (kb)
1425
1426 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1424.066 kb on + strand at 1424.066 kb on + strand at 1424.066 kb on + strand at 1424.066 kb on + strand at 1424.066 kb on + strand at 1424.066 kb on + strand at 1424.067 kb on - strand at 1424.073 kb on + strand at 1424.199 kb on - strand, within Pf1N1B4_1245 at 1424.199 kb on - strand, within Pf1N1B4_1245 at 1424.478 kb on + strand, within Pf1N1B4_1245 at 1424.478 kb on + strand, within Pf1N1B4_1245 at 1424.478 kb on + strand, within Pf1N1B4_1245 at 1424.479 kb on - strand, within Pf1N1B4_1245 at 1424.479 kb on - strand, within Pf1N1B4_1245 at 1424.658 kb on + strand, within Pf1N1B4_1245 at 1424.658 kb on + strand, within Pf1N1B4_1245 at 1424.658 kb on + strand, within Pf1N1B4_1245 at 1424.658 kb on + strand, within Pf1N1B4_1245 at 1424.659 kb on - strand, within Pf1N1B4_1245 at 1424.673 kb on + strand, within Pf1N1B4_1245 at 1424.673 kb on + strand, within Pf1N1B4_1245 at 1424.674 kb on - strand, within Pf1N1B4_1245 at 1424.674 kb on - strand, within Pf1N1B4_1245 at 1424.674 kb on - strand, within Pf1N1B4_1245 at 1424.778 kb on + strand, within Pf1N1B4_1245 at 1424.779 kb on - strand, within Pf1N1B4_1245 at 1424.865 kb on + strand, within Pf1N1B4_1245 at 1424.865 kb on + strand, within Pf1N1B4_1245 at 1424.865 kb on + strand at 1424.865 kb on + strand, within Pf1N1B4_1245 at 1424.866 kb on - strand, within Pf1N1B4_1245 at 1424.925 kb on + strand at 1424.925 kb on + strand at 1424.925 kb on + strand at 1424.926 kb on - strand at 1424.926 kb on - strand at 1424.926 kb on - strand at 1424.926 kb on - strand at 1424.926 kb on - strand at 1424.968 kb on - strand at 1425.171 kb on + strand, within Pf1N1B4_1246 at 1425.172 kb on - strand, within Pf1N1B4_1246 at 1425.172 kb on - strand, within Pf1N1B4_1246 at 1425.172 kb on - strand, within Pf1N1B4_1246 at 1425.213 kb on + strand, within Pf1N1B4_1246 at 1425.520 kb on + strand at 1425.520 kb on + strand at 1425.520 kb on + strand at 1425.520 kb on + strand at 1425.520 kb on + strand at 1425.520 kb on + strand at 1425.521 kb on - strand at 1425.521 kb on - strand at 1425.521 kb on - strand at 1425.521 kb on - strand at 1425.521 kb on - strand at 1425.572 kb on - strand at 1425.644 kb on + strand, within Pf1N1B4_1247 at 1425.644 kb on + strand, within Pf1N1B4_1247 at 1425.696 kb on + strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.697 kb on - strand, within Pf1N1B4_1247 at 1425.726 kb on - strand, within Pf1N1B4_1247 at 1425.896 kb on - strand at 1426.129 kb on - strand, within Pf1N1B4_1248 at 1426.378 kb on + strand at 1426.378 kb on + strand, within Pf1N1B4_1248 at 1426.379 kb on - strand, within Pf1N1B4_1248 at 1426.379 kb on - strand, within Pf1N1B4_1248 at 1426.379 kb on - strand, within Pf1N1B4_1248 at 1426.379 kb on - strand, within Pf1N1B4_1248
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 1,424,066 + +0.2 1,424,066 + -1.8 1,424,066 + -0.8 1,424,066 + -1.8 1,424,066 + +0.9 1,424,066 + -0.5 1,424,067 - -1.6 1,424,073 + +2.2 1,424,199 - Pf1N1B4_1245 0.17 +0.5 1,424,199 - Pf1N1B4_1245 0.17 +1.0 1,424,478 + Pf1N1B4_1245 0.47 +0.5 1,424,478 + Pf1N1B4_1245 0.47 -0.4 1,424,478 + Pf1N1B4_1245 0.47 -0.4 1,424,479 - Pf1N1B4_1245 0.47 -0.8 1,424,479 - Pf1N1B4_1245 0.47 -0.8 1,424,658 + Pf1N1B4_1245 0.66 +0.9 1,424,658 + Pf1N1B4_1245 0.66 -1.4 1,424,658 + Pf1N1B4_1245 0.66 +0.7 1,424,658 + Pf1N1B4_1245 0.66 -0.8 1,424,659 - Pf1N1B4_1245 0.66 -0.2 1,424,673 + Pf1N1B4_1245 0.68 +1.2 1,424,673 + Pf1N1B4_1245 0.68 +1.2 1,424,674 - Pf1N1B4_1245 0.68 -0.8 1,424,674 - Pf1N1B4_1245 0.68 -0.5 1,424,674 - Pf1N1B4_1245 0.68 -1.1 1,424,778 + Pf1N1B4_1245 0.79 -0.2 1,424,779 - Pf1N1B4_1245 0.79 +0.3 1,424,865 + Pf1N1B4_1245 0.89 -2.2 1,424,865 + Pf1N1B4_1245 0.89 -0.2 1,424,865 + +0.2 1,424,865 + Pf1N1B4_1245 0.89 -0.3 1,424,866 - Pf1N1B4_1245 0.89 -0.8 1,424,925 + +1.2 1,424,925 + +0.8 1,424,925 + -0.1 1,424,926 - -2.0 1,424,926 - -1.4 1,424,926 - +1.8 1,424,926 - +0.2 1,424,926 - -0.2 1,424,968 - -1.2 1,425,171 + Pf1N1B4_1246 0.29 +0.2 1,425,172 - Pf1N1B4_1246 0.29 -0.1 1,425,172 - Pf1N1B4_1246 0.29 -0.4 1,425,172 - Pf1N1B4_1246 0.29 -0.4 1,425,213 + Pf1N1B4_1246 0.39 -1.4 1,425,520 + +0.8 1,425,520 + -1.4 1,425,520 + +1.8 1,425,520 + -0.6 1,425,520 + +0.2 1,425,520 + +0.2 1,425,521 - +0.1 1,425,521 - -1.4 1,425,521 - +0.8 1,425,521 - +0.5 1,425,521 - +1.5 1,425,572 - -0.7 1,425,644 + Pf1N1B4_1247 0.25 +0.9 1,425,644 + Pf1N1B4_1247 0.25 +0.6 1,425,696 + Pf1N1B4_1247 0.40 -0.6 1,425,697 - Pf1N1B4_1247 0.40 +0.6 1,425,697 - Pf1N1B4_1247 0.40 -1.0 1,425,697 - Pf1N1B4_1247 0.40 +0.2 1,425,697 - Pf1N1B4_1247 0.40 +0.2 1,425,697 - Pf1N1B4_1247 0.40 -0.6 1,425,697 - Pf1N1B4_1247 0.40 +2.2 1,425,697 - Pf1N1B4_1247 0.40 +0.2 1,425,697 - Pf1N1B4_1247 0.40 -1.6 1,425,726 - Pf1N1B4_1247 0.49 +0.2 1,425,896 - +0.9 1,426,129 - Pf1N1B4_1248 0.29 -0.8 1,426,378 + +1.8 1,426,378 + Pf1N1B4_1248 0.65 -0.5 1,426,379 - Pf1N1B4_1248 0.65 -0.3 1,426,379 - Pf1N1B4_1248 0.65 -0.8 1,426,379 - Pf1N1B4_1248 0.65 +2.5 1,426,379 - Pf1N1B4_1248 0.65 -0.3
Or see this region's nucleotide sequence