Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1235 and Pf1N1B4_1236 overlap by 4 nucleotides Pf1N1B4_1236 and Pf1N1B4_5178 are separated by 154 nucleotides Pf1N1B4_5178 and Pf1N1B4_5179 are separated by 171 nucleotides
Pf1N1B4_1235 - Exodeoxyribonuclease V beta chain (EC 3.1.11.5), at 1,402,162 to 1,405,851
_1235
Pf1N1B4_1236 - Exodeoxyribonuclease V gamma chain (EC 3.1.11.5), at 1,405,848 to 1,409,300
_1236
Pf1N1B4_5178 - 5S RNA, at 1,409,455 to 1,409,574
_5178
Pf1N1B4_5179 - Large Subunit Ribosomal RNA; lsuRNA; LSU rRNA, at 1,409,746 to 1,412,637
_5179
Position (kb)
1405
1406
1407
1408
1409
1410 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1404.998 kb on - strand, within Pf1N1B4_1235 at 1405.377 kb on + strand, within Pf1N1B4_1235 at 1405.629 kb on + strand at 1405.749 kb on - strand at 1405.761 kb on + strand at 1405.761 kb on + strand at 1405.761 kb on + strand at 1406.030 kb on - strand at 1406.030 kb on - strand at 1406.098 kb on - strand at 1406.225 kb on + strand, within Pf1N1B4_1236 at 1406.403 kb on + strand, within Pf1N1B4_1236 at 1406.580 kb on + strand, within Pf1N1B4_1236 at 1406.739 kb on + strand, within Pf1N1B4_1236 at 1406.740 kb on - strand, within Pf1N1B4_1236 at 1406.797 kb on + strand, within Pf1N1B4_1236 at 1406.874 kb on + strand, within Pf1N1B4_1236 at 1406.874 kb on + strand, within Pf1N1B4_1236 at 1406.875 kb on - strand, within Pf1N1B4_1236 at 1406.896 kb on + strand, within Pf1N1B4_1236 at 1406.897 kb on - strand, within Pf1N1B4_1236 at 1407.067 kb on + strand, within Pf1N1B4_1236 at 1407.067 kb on + strand, within Pf1N1B4_1236 at 1407.067 kb on + strand, within Pf1N1B4_1236 at 1407.068 kb on - strand, within Pf1N1B4_1236 at 1407.068 kb on - strand, within Pf1N1B4_1236 at 1407.068 kb on - strand, within Pf1N1B4_1236 at 1407.068 kb on - strand, within Pf1N1B4_1236 at 1407.354 kb on + strand, within Pf1N1B4_1236 at 1407.430 kb on + strand at 1407.463 kb on - strand, within Pf1N1B4_1236 at 1407.546 kb on - strand, within Pf1N1B4_1236 at 1407.799 kb on - strand, within Pf1N1B4_1236 at 1408.006 kb on + strand, within Pf1N1B4_1236 at 1408.006 kb on + strand, within Pf1N1B4_1236 at 1408.006 kb on + strand at 1408.007 kb on - strand, within Pf1N1B4_1236 at 1408.007 kb on - strand, within Pf1N1B4_1236 at 1408.095 kb on + strand at 1408.096 kb on - strand at 1408.294 kb on + strand, within Pf1N1B4_1236 at 1408.299 kb on - strand, within Pf1N1B4_1236 at 1408.299 kb on - strand, within Pf1N1B4_1236 at 1408.299 kb on - strand at 1408.328 kb on + strand, within Pf1N1B4_1236 at 1408.328 kb on + strand, within Pf1N1B4_1236 at 1408.334 kb on - strand, within Pf1N1B4_1236 at 1408.442 kb on - strand, within Pf1N1B4_1236 at 1408.880 kb on + strand, within Pf1N1B4_1236 at 1408.880 kb on + strand, within Pf1N1B4_1236 at 1408.885 kb on - strand, within Pf1N1B4_1236 at 1408.885 kb on - strand, within Pf1N1B4_1236 at 1408.885 kb on - strand, within Pf1N1B4_1236 at 1409.167 kb on - strand at 1409.267 kb on + strand at 1409.267 kb on + strand at 1409.268 kb on - strand at 1409.268 kb on - strand at 1409.300 kb on + strand at 1409.301 kb on - strand at 1409.301 kb on - strand at 1409.301 kb on - strand at 1409.320 kb on + strand at 1409.330 kb on - strand at 1409.342 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 1,404,998 - Pf1N1B4_1235 0.77 -1.8 1,405,377 + Pf1N1B4_1235 0.87 -2.2 1,405,629 + -1.0 1,405,749 - -2.2 1,405,761 + -0.8 1,405,761 + +0.2 1,405,761 + -2.8 1,406,030 - +1.2 1,406,030 - -2.4 1,406,098 - -0.4 1,406,225 + Pf1N1B4_1236 0.11 -0.7 1,406,403 + Pf1N1B4_1236 0.16 -1.2 1,406,580 + Pf1N1B4_1236 0.21 -0.8 1,406,739 + Pf1N1B4_1236 0.26 -2.2 1,406,740 - Pf1N1B4_1236 0.26 -0.3 1,406,797 + Pf1N1B4_1236 0.27 +1.7 1,406,874 + Pf1N1B4_1236 0.30 +0.2 1,406,874 + Pf1N1B4_1236 0.30 -1.4 1,406,875 - Pf1N1B4_1236 0.30 +0.3 1,406,896 + Pf1N1B4_1236 0.30 -1.4 1,406,897 - Pf1N1B4_1236 0.30 -0.8 1,407,067 + Pf1N1B4_1236 0.35 -0.4 1,407,067 + Pf1N1B4_1236 0.35 -0.8 1,407,067 + Pf1N1B4_1236 0.35 -0.8 1,407,068 - Pf1N1B4_1236 0.35 -1.5 1,407,068 - Pf1N1B4_1236 0.35 -1.8 1,407,068 - Pf1N1B4_1236 0.35 -0.8 1,407,068 - Pf1N1B4_1236 0.35 -0.4 1,407,354 + Pf1N1B4_1236 0.44 -1.8 1,407,430 + +1.2 1,407,463 - Pf1N1B4_1236 0.47 +0.2 1,407,546 - Pf1N1B4_1236 0.49 +0.2 1,407,799 - Pf1N1B4_1236 0.57 -0.4 1,408,006 + Pf1N1B4_1236 0.62 -1.8 1,408,006 + Pf1N1B4_1236 0.62 +0.2 1,408,006 + +1.2 1,408,007 - Pf1N1B4_1236 0.63 -0.4 1,408,007 - Pf1N1B4_1236 0.63 +0.2 1,408,095 + -0.8 1,408,096 - -0.8 1,408,294 + Pf1N1B4_1236 0.71 -0.8 1,408,299 - Pf1N1B4_1236 0.71 -0.7 1,408,299 - Pf1N1B4_1236 0.71 -1.4 1,408,299 - -0.8 1,408,328 + Pf1N1B4_1236 0.72 -1.4 1,408,328 + Pf1N1B4_1236 0.72 -1.4 1,408,334 - Pf1N1B4_1236 0.72 -0.7 1,408,442 - Pf1N1B4_1236 0.75 +0.2 1,408,880 + Pf1N1B4_1236 0.88 -1.4 1,408,880 + Pf1N1B4_1236 0.88 -2.2 1,408,885 - Pf1N1B4_1236 0.88 -1.1 1,408,885 - Pf1N1B4_1236 0.88 +0.2 1,408,885 - Pf1N1B4_1236 0.88 -0.3 1,409,167 - -0.4 1,409,267 + +0.2 1,409,267 + +0.4 1,409,268 - +0.9 1,409,268 - -0.8 1,409,300 + +0.2 1,409,301 - +0.3 1,409,301 - +0.7 1,409,301 - +1.2 1,409,320 + -2.4 1,409,330 - +1.0 1,409,342 + -0.3
Or see this region's nucleotide sequence