Experiment: Phosphomycin 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_2137 and Ac3H11_2138 are separated by 30 nucleotides Ac3H11_2138 and Ac3H11_2139 overlap by 23 nucleotides Ac3H11_2139 and Ac3H11_2140 are separated by 150 nucleotides Ac3H11_2140 and Ac3H11_2141 are separated by 169 nucleotides
Ac3H11_2137 - Translation elongation factor Tu, at 367,505 to 368,995
_2137
Ac3H11_2138 - hypothetical protein, at 369,026 to 369,160
_2138
Ac3H11_2139 - Translation elongation factor G, at 369,138 to 371,240
_2139
Ac3H11_2140 - SSU ribosomal protein S7p (S5e), at 371,391 to 371,864
_2140
Ac3H11_2141 - SSU ribosomal protein S12p (S23e), at 372,034 to 372,411
_2141
Position (kb) 369
370
371
372 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 369.136 kb on - strand, within Ac3H11_2138 at 369.136 kb on - strand, within Ac3H11_2138 at 369.136 kb on - strand, within Ac3H11_2138 at 369.136 kb on - strand, within Ac3H11_2138 at 369.136 kb on - strand, within Ac3H11_2138 at 369.136 kb on - strand, within Ac3H11_2138 at 369.136 kb on - strand, within Ac3H11_2138 at 369.136 kb on - strand, within Ac3H11_2138 at 369.140 kb on - strand at 369.140 kb on - strand at 369.140 kb on - strand at 369.140 kb on - strand at 369.140 kb on - strand at 369.140 kb on - strand at 369.140 kb on - strand at 369.140 kb on - strand at 369.140 kb on - strand at 369.140 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.02 mg/ml remove 369,136 - Ac3H11_2138 0.81 -1.5 369,136 - Ac3H11_2138 0.81 -4.0 369,136 - Ac3H11_2138 0.81 -2.5 369,136 - Ac3H11_2138 0.81 -0.3 369,136 - Ac3H11_2138 0.81 -1.3 369,136 - Ac3H11_2138 0.81 -0.7 369,136 - Ac3H11_2138 0.81 -3.7 369,136 - Ac3H11_2138 0.81 +0.5 369,140 - -0.7 369,140 - -0.6 369,140 - -2.4 369,140 - -0.8 369,140 - -0.2 369,140 - -1.0 369,140 - -0.9 369,140 - -0.2 369,140 - -1.6 369,140 - -1.2
Or see this region's nucleotide sequence