Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gmk and rpoZ are separated by 58 nucleotides rpoZ and Shewana3_3812 are separated by 43 nucleotides Shewana3_3812 and Shewana3_3813 are separated by 22 nucleotides Shewana3_3813 and Shewana3_3814 are separated by 232 nucleotides
Shewana3_3810: gmk - guanylate kinase (RefSeq), at 4,576,589 to 4,577,212
gmk
Shewana3_3811: rpoZ - DNA-directed RNA polymerase subunit omega (RefSeq), at 4,577,271 to 4,577,549
rpoZ
Shewana3_3812: Shewana3_3812 - bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (RefSeq), at 4,577,593 to 4,579,695
_3812
Shewana3_3813: Shewana3_3813 - putative endoribonuclease L-PSP (RefSeq), at 4,579,718 to 4,580,101
_3813
Shewana3_3814: Shewana3_3814 - AMP-dependent synthetase and ligase (RefSeq), at 4,580,334 to 4,581,998
_3814
Position (kb)
4577
4578
4579
4580 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 4577.162 kb on + strand at 4577.286 kb on + strand at 4577.369 kb on + strand, within rpoZ at 4577.474 kb on + strand, within rpoZ at 4577.558 kb on - strand at 4577.564 kb on + strand at 4579.265 kb on - strand, within Shewana3_3812 at 4579.437 kb on + strand, within Shewana3_3812 at 4579.502 kb on - strand at 4579.592 kb on + strand at 4579.836 kb on + strand, within Shewana3_3813 at 4579.836 kb on + strand, within Shewana3_3813 at 4580.001 kb on + strand, within Shewana3_3813 at 4580.017 kb on + strand, within Shewana3_3813 at 4580.017 kb on + strand, within Shewana3_3813 at 4580.025 kb on - strand, within Shewana3_3813 at 4580.025 kb on - strand, within Shewana3_3813 at 4580.025 kb on - strand, within Shewana3_3813 at 4580.056 kb on + strand, within Shewana3_3813 at 4580.064 kb on - strand at 4580.166 kb on + strand at 4580.166 kb on + strand at 4580.174 kb on - strand at 4580.184 kb on + strand at 4580.402 kb on - strand at 4580.500 kb on + strand at 4580.508 kb on - strand, within Shewana3_3814 at 4580.508 kb on - strand, within Shewana3_3814 at 4580.552 kb on + strand, within Shewana3_3814 at 4580.573 kb on + strand, within Shewana3_3814
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 4,577,162 + -0.2 4,577,286 + +0.9 4,577,369 + rpoZ Shewana3_3811 0.35 +4.9 4,577,474 + rpoZ Shewana3_3811 0.73 +0.8 4,577,558 - -0.9 4,577,564 + -0.0 4,579,265 - Shewana3_3812 0.80 +3.6 4,579,437 + Shewana3_3812 0.88 +1.5 4,579,502 - +4.1 4,579,592 + +1.6 4,579,836 + Shewana3_3813 0.31 +0.5 4,579,836 + Shewana3_3813 0.31 +0.2 4,580,001 + Shewana3_3813 0.74 -0.1 4,580,017 + Shewana3_3813 0.78 -0.5 4,580,017 + Shewana3_3813 0.78 +1.5 4,580,025 - Shewana3_3813 0.80 -1.5 4,580,025 - Shewana3_3813 0.80 -0.3 4,580,025 - Shewana3_3813 0.80 -0.3 4,580,056 + Shewana3_3813 0.88 +0.3 4,580,064 - +0.1 4,580,166 + -0.4 4,580,166 + -0.5 4,580,174 - -1.0 4,580,184 + -0.9 4,580,402 - +1.0 4,580,500 + +0.7 4,580,508 - Shewana3_3814 0.10 +0.8 4,580,508 - Shewana3_3814 0.10 -2.0 4,580,552 + Shewana3_3814 0.13 -0.4 4,580,573 + Shewana3_3814 0.14 -1.1
Or see this region's nucleotide sequence