Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2578 and Shewana3_2579 are separated by 233 nucleotides Shewana3_2579 and Shewana3_2580 are separated by 58 nucleotides Shewana3_2580 and Shewana3_2581 are separated by 165 nucleotides Shewana3_2581 and Shewana3_2582 are separated by 130 nucleotides Shewana3_2582 and lpxK overlap by 11 nucleotides
Shewana3_2578: Shewana3_2578 - hypothetical protein (RefSeq), at 3,080,936 to 3,084,166
_2578
Shewana3_2579: Shewana3_2579 - glutaredoxin (RefSeq), at 3,084,400 to 3,084,756
_2579
Shewana3_2580: Shewana3_2580 - hypothetical protein (RefSeq), at 3,084,815 to 3,085,504
_2580
Shewana3_2581: Shewana3_2581 - putative lipoprotein (RefSeq), at 3,085,670 to 3,086,134
_2581
Shewana3_2582: Shewana3_2582 - hypothetical protein (RefSeq), at 3,086,265 to 3,086,444
_2582
Shewana3_2583: lpxK - tetraacyldisaccharide 4'-kinase (RefSeq), at 3,086,434 to 3,087,441
lpxK
Position (kb)
3084
3085
3086 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3083.848 kb on + strand at 3083.937 kb on + strand at 3083.979 kb on - strand at 3084.039 kb on - strand at 3084.274 kb on + strand at 3084.282 kb on + strand at 3084.288 kb on - strand at 3084.288 kb on - strand at 3084.458 kb on + strand, within Shewana3_2579 at 3084.466 kb on - strand, within Shewana3_2579 at 3084.554 kb on + strand, within Shewana3_2579 at 3084.562 kb on - strand, within Shewana3_2579 at 3084.643 kb on + strand, within Shewana3_2579 at 3085.116 kb on + strand, within Shewana3_2580 at 3085.244 kb on + strand at 3085.267 kb on - strand, within Shewana3_2580 at 3085.363 kb on - strand, within Shewana3_2580 at 3085.574 kb on - strand at 3085.664 kb on - strand at 3085.707 kb on + strand at 3085.803 kb on + strand, within Shewana3_2581 at 3085.940 kb on - strand, within Shewana3_2581 at 3086.128 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 3,083,848 + -1.4 3,083,937 + -0.8 3,083,979 - +1.3 3,084,039 - +1.6 3,084,274 + +1.3 3,084,282 + -1.9 3,084,288 - +1.0 3,084,288 - +1.5 3,084,458 + Shewana3_2579 0.16 -0.3 3,084,466 - Shewana3_2579 0.18 +0.6 3,084,554 + Shewana3_2579 0.43 -1.1 3,084,562 - Shewana3_2579 0.45 +0.0 3,084,643 + Shewana3_2579 0.68 -1.0 3,085,116 + Shewana3_2580 0.44 +0.1 3,085,244 + +3.6 3,085,267 - Shewana3_2580 0.66 +0.1 3,085,363 - Shewana3_2580 0.79 +1.6 3,085,574 - +0.6 3,085,664 - -0.6 3,085,707 + +0.2 3,085,803 + Shewana3_2581 0.29 +1.0 3,085,940 - Shewana3_2581 0.58 -0.8 3,086,128 + +1.0
Or see this region's nucleotide sequence