Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sbcB and Shewana3_2573 are separated by 212 nucleotides Shewana3_2573 and Shewana3_2574 are separated by 83 nucleotides Shewana3_2574 and Shewana3_2575 overlap by 20 nucleotides Shewana3_2575 and Shewana3_2576 are separated by 73 nucleotides
Shewana3_2572: sbcB - exonuclease I (RefSeq), at 3,076,054 to 3,077,466
sbcB
Shewana3_2573: Shewana3_2573 - Cytidine deaminase (EC 3.5.4.5) (from data) , at 3,077,679 to 3,078,569
_2573
Shewana3_2574: Shewana3_2574 - hypothetical protein (RefSeq), at 3,078,653 to 3,078,868
_2574
Shewana3_2575: Shewana3_2575 - glyoxalase/bleomycin resistance protein/dioxygenase (RefSeq), at 3,078,849 to 3,079,196
_2575
Shewana3_2576: Shewana3_2576 - hypothetical protein (RefSeq), at 3,079,270 to 3,079,974
_2576
Position (kb)
3077
3078
3079 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3076.690 kb on - strand, within sbcB at 3076.762 kb on + strand, within sbcB at 3076.762 kb on + strand, within sbcB at 3077.146 kb on - strand, within sbcB at 3077.701 kb on + strand at 3077.935 kb on + strand, within Shewana3_2573 at 3077.941 kb on + strand, within Shewana3_2573 at 3077.941 kb on + strand, within Shewana3_2573 at 3077.949 kb on - strand, within Shewana3_2573 at 3077.966 kb on + strand, within Shewana3_2573 at 3077.966 kb on + strand, within Shewana3_2573 at 3077.974 kb on - strand, within Shewana3_2573 at 3078.033 kb on + strand, within Shewana3_2573 at 3078.144 kb on - strand, within Shewana3_2573 at 3078.144 kb on - strand, within Shewana3_2573 at 3078.151 kb on + strand, within Shewana3_2573 at 3078.276 kb on + strand, within Shewana3_2573 at 3078.284 kb on - strand, within Shewana3_2573 at 3078.374 kb on + strand, within Shewana3_2573 at 3078.469 kb on + strand, within Shewana3_2573 at 3078.584 kb on + strand at 3078.653 kb on - strand at 3078.851 kb on + strand at 3079.281 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 3,076,690 - sbcB Shewana3_2572 0.45 +0.4 3,076,762 + sbcB Shewana3_2572 0.50 +0.6 3,076,762 + sbcB Shewana3_2572 0.50 -1.8 3,077,146 - sbcB Shewana3_2572 0.77 +2.0 3,077,701 + +1.9 3,077,935 + Shewana3_2573 0.29 -0.4 3,077,941 + Shewana3_2573 0.29 -0.6 3,077,941 + Shewana3_2573 0.29 -0.1 3,077,949 - Shewana3_2573 0.30 +0.3 3,077,966 + Shewana3_2573 0.32 +0.4 3,077,966 + Shewana3_2573 0.32 +2.0 3,077,974 - Shewana3_2573 0.33 -0.6 3,078,033 + Shewana3_2573 0.40 -2.7 3,078,144 - Shewana3_2573 0.52 -0.9 3,078,144 - Shewana3_2573 0.52 -0.3 3,078,151 + Shewana3_2573 0.53 -1.3 3,078,276 + Shewana3_2573 0.67 -0.1 3,078,284 - Shewana3_2573 0.68 -0.2 3,078,374 + Shewana3_2573 0.78 -1.6 3,078,469 + Shewana3_2573 0.89 +1.3 3,078,584 + -0.5 3,078,653 - +0.0 3,078,851 + +1.6 3,079,281 - +0.2
Or see this region's nucleotide sequence