Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2509 and Shewana3_2510 are separated by 296 nucleotides Shewana3_2510 and Shewana3_2511 are separated by 69 nucleotides Shewana3_2511 and Shewana3_2512 are separated by 324 nucleotides
Shewana3_2509: Shewana3_2509 - LuxR family transcriptional regulator (RefSeq), at 2,999,802 to 3,000,437
_2509
Shewana3_2510: Shewana3_2510 - cytochrome B561 (RefSeq), at 3,000,734 to 3,001,480
_2510
Shewana3_2511: Shewana3_2511 - cytochrome c3 (RefSeq), at 3,001,550 to 3,001,900
_2511
Shewana3_2512: Shewana3_2512 - heat shock protein HtpX (RefSeq), at 3,002,225 to 3,003,088
_2512
Position (kb)
3000
3001
3002 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3000.056 kb on + strand, within Shewana3_2509 at 3000.079 kb on + strand, within Shewana3_2509 at 3000.097 kb on - strand, within Shewana3_2509 at 3000.454 kb on - strand at 3000.532 kb on + strand at 3000.550 kb on - strand at 3000.557 kb on + strand at 3000.744 kb on - strand at 3000.815 kb on + strand, within Shewana3_2510 at 3000.907 kb on + strand, within Shewana3_2510 at 3001.013 kb on - strand, within Shewana3_2510 at 3001.058 kb on + strand, within Shewana3_2510 at 3001.168 kb on - strand, within Shewana3_2510 at 3001.251 kb on + strand, within Shewana3_2510 at 3001.259 kb on - strand, within Shewana3_2510 at 3001.284 kb on - strand, within Shewana3_2510 at 3001.329 kb on + strand, within Shewana3_2510 at 3001.465 kb on + strand at 3001.648 kb on - strand, within Shewana3_2511 at 3001.833 kb on - strand, within Shewana3_2511 at 3001.855 kb on + strand, within Shewana3_2511 at 3001.910 kb on + strand at 3001.952 kb on - strand at 3002.156 kb on - strand at 3002.190 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 3,000,056 + Shewana3_2509 0.40 +2.7 3,000,079 + Shewana3_2509 0.44 +2.7 3,000,097 - Shewana3_2509 0.46 -1.7 3,000,454 - +0.9 3,000,532 + +0.5 3,000,550 - +3.7 3,000,557 + +3.7 3,000,744 - +0.7 3,000,815 + Shewana3_2510 0.11 +0.4 3,000,907 + Shewana3_2510 0.23 +0.4 3,001,013 - Shewana3_2510 0.37 +2.7 3,001,058 + Shewana3_2510 0.43 +0.4 3,001,168 - Shewana3_2510 0.58 -2.2 3,001,251 + Shewana3_2510 0.69 -0.9 3,001,259 - Shewana3_2510 0.70 -0.2 3,001,284 - Shewana3_2510 0.74 -0.0 3,001,329 + Shewana3_2510 0.80 +0.4 3,001,465 + +0.9 3,001,648 - Shewana3_2511 0.28 +0.8 3,001,833 - Shewana3_2511 0.81 +0.3 3,001,855 + Shewana3_2511 0.87 +0.1 3,001,910 + -0.5 3,001,952 - +0.2 3,002,156 - +0.8 3,002,190 + +2.7
Or see this region's nucleotide sequence