Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2497 and Shewana3_2498 are separated by 90 nucleotides Shewana3_2498 and Shewana3_2499 are separated by 68 nucleotides Shewana3_2499 and Shewana3_2500 are separated by 95 nucleotides
Shewana3_2497: Shewana3_2497 - aminoglycoside phosphotransferase (RefSeq), at 2,987,714 to 2,989,018
_2497
Shewana3_2498: Shewana3_2498 - nicotinamide mononucleotide transporter PnuC (RefSeq), at 2,989,109 to 2,989,774
_2498
Shewana3_2499: Shewana3_2499 - hypothetical protein (RefSeq), at 2,989,843 to 2,990,103
_2499
Shewana3_2500: Shewana3_2500 - TonB-dependent receptor (RefSeq), at 2,990,199 to 2,992,472
_2500
Position (kb)
2989
2990
2991 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2989.393 kb on - strand, within Shewana3_2498 at 2989.624 kb on - strand, within Shewana3_2498 at 2989.676 kb on + strand, within Shewana3_2498 at 2989.751 kb on - strand at 2989.776 kb on + strand at 2989.854 kb on + strand at 2989.930 kb on - strand, within Shewana3_2499 at 2989.942 kb on - strand, within Shewana3_2499 at 2990.600 kb on - strand, within Shewana3_2500 at 2990.600 kb on - strand, within Shewana3_2500 at 2990.600 kb on - strand, within Shewana3_2500 at 2990.610 kb on - strand, within Shewana3_2500 at 2990.759 kb on - strand, within Shewana3_2500 at 2990.792 kb on + strand, within Shewana3_2500 at 2990.800 kb on - strand, within Shewana3_2500 at 2990.805 kb on - strand, within Shewana3_2500 at 2990.817 kb on - strand, within Shewana3_2500 at 2990.817 kb on - strand, within Shewana3_2500 at 2990.911 kb on - strand, within Shewana3_2500 at 2990.947 kb on + strand, within Shewana3_2500 at 2990.948 kb on - strand, within Shewana3_2500 at 2990.955 kb on - strand, within Shewana3_2500 at 2990.955 kb on - strand, within Shewana3_2500 at 2991.064 kb on - strand, within Shewana3_2500
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 2,989,393 - Shewana3_2498 0.43 +1.1 2,989,624 - Shewana3_2498 0.77 +0.4 2,989,676 + Shewana3_2498 0.85 +3.7 2,989,751 - -1.5 2,989,776 + +1.1 2,989,854 + +0.9 2,989,930 - Shewana3_2499 0.33 +1.1 2,989,942 - Shewana3_2499 0.38 +1.1 2,990,600 - Shewana3_2500 0.18 -0.4 2,990,600 - Shewana3_2500 0.18 -2.4 2,990,600 - Shewana3_2500 0.18 -1.9 2,990,610 - Shewana3_2500 0.18 -0.7 2,990,759 - Shewana3_2500 0.25 +0.1 2,990,792 + Shewana3_2500 0.26 +2.7 2,990,800 - Shewana3_2500 0.26 +2.3 2,990,805 - Shewana3_2500 0.27 -1.6 2,990,817 - Shewana3_2500 0.27 -0.9 2,990,817 - Shewana3_2500 0.27 +0.2 2,990,911 - Shewana3_2500 0.31 -0.4 2,990,947 + Shewana3_2500 0.33 +2.4 2,990,948 - Shewana3_2500 0.33 -0.1 2,990,955 - Shewana3_2500 0.33 -0.9 2,990,955 - Shewana3_2500 0.33 +0.1 2,991,064 - Shewana3_2500 0.38 +2.1
Or see this region's nucleotide sequence