Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2443 and recR are separated by 317 nucleotides recR and Shewana3_2445 are separated by 12 nucleotides Shewana3_2445 and Shewana3_2446 are separated by 163 nucleotides
Shewana3_2443: Shewana3_2443 - heat shock protein 90 (RefSeq), at 2,929,409 to 2,931,322
_2443
Shewana3_2444: recR - recombination protein RecR (RefSeq), at 2,931,640 to 2,932,239
recR
Shewana3_2445: Shewana3_2445 - hypothetical protein (RefSeq), at 2,932,252 to 2,932,587
_2445
Shewana3_2446: Shewana3_2446 - DNA polymerase III, subunits gamma and tau (RefSeq), at 2,932,751 to 2,935,873
_2446
Position (kb)
2931
2932
2933 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2930.665 kb on - strand, within Shewana3_2443 at 2930.719 kb on - strand, within Shewana3_2443 at 2930.785 kb on + strand, within Shewana3_2443 at 2930.875 kb on + strand, within Shewana3_2443 at 2930.901 kb on - strand, within Shewana3_2443 at 2930.999 kb on - strand, within Shewana3_2443 at 2931.073 kb on - strand, within Shewana3_2443 at 2931.073 kb on - strand, within Shewana3_2443 at 2931.109 kb on - strand, within Shewana3_2443 at 2931.185 kb on - strand at 2931.491 kb on + strand at 2931.534 kb on + strand at 2931.548 kb on + strand at 2931.548 kb on + strand at 2931.556 kb on - strand at 2931.667 kb on - strand at 2931.669 kb on + strand at 2931.669 kb on + strand at 2931.672 kb on - strand at 2931.677 kb on - strand at 2931.784 kb on + strand, within recR at 2931.876 kb on - strand, within recR at 2931.884 kb on - strand, within recR at 2931.884 kb on - strand, within recR at 2931.884 kb on - strand, within recR at 2932.062 kb on - strand, within recR at 2932.099 kb on - strand, within recR at 2932.099 kb on - strand, within recR at 2932.206 kb on + strand at 2932.356 kb on - strand, within Shewana3_2445 at 2932.623 kb on - strand at 2932.623 kb on - strand at 2932.731 kb on + strand at 2932.758 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 2,930,665 - Shewana3_2443 0.66 +2.0 2,930,719 - Shewana3_2443 0.68 +3.7 2,930,785 + Shewana3_2443 0.72 +1.5 2,930,875 + Shewana3_2443 0.77 +3.0 2,930,901 - Shewana3_2443 0.78 +2.0 2,930,999 - Shewana3_2443 0.83 +1.3 2,931,073 - Shewana3_2443 0.87 +1.7 2,931,073 - Shewana3_2443 0.87 +2.8 2,931,109 - Shewana3_2443 0.89 +2.1 2,931,185 - +1.0 2,931,491 + -0.8 2,931,534 + -0.7 2,931,548 + -0.8 2,931,548 + +0.1 2,931,556 - +2.0 2,931,667 - -0.7 2,931,669 + +3.0 2,931,669 + +1.4 2,931,672 - -2.5 2,931,677 - -0.1 2,931,784 + recR Shewana3_2444 0.24 +2.4 2,931,876 - recR Shewana3_2444 0.39 -1.4 2,931,884 - recR Shewana3_2444 0.41 -0.0 2,931,884 - recR Shewana3_2444 0.41 -3.1 2,931,884 - recR Shewana3_2444 0.41 -0.3 2,932,062 - recR Shewana3_2444 0.70 -0.5 2,932,099 - recR Shewana3_2444 0.77 -2.9 2,932,099 - recR Shewana3_2444 0.77 -1.4 2,932,206 + +4.0 2,932,356 - Shewana3_2445 0.31 -1.7 2,932,623 - +0.6 2,932,623 - +3.0 2,932,731 + -0.6 2,932,758 + +0.8
Or see this region's nucleotide sequence