Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2380 and Shewana3_2381 are separated by 24 nucleotides Shewana3_2381 and minC are separated by 123 nucleotides minC and Shewana3_2383 are separated by 27 nucleotides Shewana3_2383 and minE are separated by 2 nucleotides
Shewana3_2380: Shewana3_2380 - putative transglycosylase (RefSeq), at 2,860,378 to 2,861,379
_2380
Shewana3_2381: Shewana3_2381 - hypothetical protein (RefSeq), at 2,861,404 to 2,861,682
_2381
Shewana3_2382: minC - septum formation inhibitor (RefSeq), at 2,861,806 to 2,862,471
minC
Shewana3_2383: Shewana3_2383 - septum site-determining protein MinD (RefSeq), at 2,862,499 to 2,863,308
_2383
Shewana3_2384: minE - cell division topological specificity factor MinE (RefSeq), at 2,863,311 to 2,863,568
minE
Position (kb)
2861
2862
2863 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2860.930 kb on + strand, within Shewana3_2380 at 2860.968 kb on + strand at 2860.976 kb on - strand, within Shewana3_2380 at 2860.976 kb on - strand, within Shewana3_2380 at 2860.976 kb on - strand, within Shewana3_2380 at 2860.976 kb on - strand, within Shewana3_2380 at 2860.976 kb on - strand, within Shewana3_2380 at 2860.988 kb on - strand, within Shewana3_2380 at 2861.250 kb on - strand, within Shewana3_2380 at 2861.267 kb on + strand, within Shewana3_2380 at 2861.275 kb on - strand, within Shewana3_2380 at 2861.506 kb on - strand, within Shewana3_2381 at 2861.642 kb on + strand, within Shewana3_2381 at 2861.731 kb on + strand at 2861.739 kb on - strand at 2861.808 kb on + strand at 2861.847 kb on - strand at 2862.088 kb on - strand, within minC at 2862.088 kb on - strand, within minC at 2862.399 kb on + strand, within minC at 2862.490 kb on + strand at 2862.525 kb on + strand at 2862.926 kb on + strand, within Shewana3_2383 at 2863.079 kb on + strand, within Shewana3_2383
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 2,860,930 + Shewana3_2380 0.55 +2.0 2,860,968 + +2.4 2,860,976 - Shewana3_2380 0.60 +1.0 2,860,976 - Shewana3_2380 0.60 -0.4 2,860,976 - Shewana3_2380 0.60 -0.5 2,860,976 - Shewana3_2380 0.60 -0.0 2,860,976 - Shewana3_2380 0.60 +1.1 2,860,988 - Shewana3_2380 0.61 -2.4 2,861,250 - Shewana3_2380 0.87 +0.4 2,861,267 + Shewana3_2380 0.89 +2.0 2,861,275 - Shewana3_2380 0.90 +0.1 2,861,506 - Shewana3_2381 0.37 -0.3 2,861,642 + Shewana3_2381 0.85 +3.0 2,861,731 + -1.5 2,861,739 - +2.0 2,861,808 + +1.7 2,861,847 - +2.0 2,862,088 - minC Shewana3_2382 0.42 +2.0 2,862,088 - minC Shewana3_2382 0.42 +4.0 2,862,399 + minC Shewana3_2382 0.89 +1.3 2,862,490 + -1.8 2,862,525 + +0.5 2,862,926 + Shewana3_2383 0.53 +0.2 2,863,079 + Shewana3_2383 0.72 +0.4
Or see this region's nucleotide sequence