Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2347 and Shewana3_2348 are separated by 44 nucleotides Shewana3_2348 and Shewana3_2349 overlap by 8 nucleotides Shewana3_2349 and Shewana3_2350 are separated by 18 nucleotides Shewana3_2350 and Shewana3_2351 are separated by 60 nucleotides
Shewana3_2347: Shewana3_2347 - RND family efflux transporter MFP subunit (RefSeq), at 2,825,448 to 2,826,572
_2347
Shewana3_2348: Shewana3_2348 - cytochrome c biogenesis protein, transmembrane region (RefSeq), at 2,826,617 to 2,827,315
_2348
Shewana3_2349: Shewana3_2349 - hypothetical protein (RefSeq), at 2,827,308 to 2,827,841
_2349
Shewana3_2350: Shewana3_2350 - redox-active disulfide protein 2 (RefSeq), at 2,827,860 to 2,828,102
_2350
Shewana3_2351: Shewana3_2351 - ArsR family transcriptional regulator (RefSeq), at 2,828,163 to 2,828,471
_2351
Position (kb)
2827
2828 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2826.317 kb on + strand, within Shewana3_2347 at 2826.594 kb on - strand at 2826.930 kb on - strand, within Shewana3_2348 at 2827.176 kb on - strand, within Shewana3_2348 at 2827.277 kb on + strand at 2827.430 kb on + strand, within Shewana3_2349 at 2827.458 kb on + strand, within Shewana3_2349 at 2827.500 kb on + strand, within Shewana3_2349 at 2827.656 kb on - strand, within Shewana3_2349 at 2827.782 kb on + strand, within Shewana3_2349 at 2827.782 kb on + strand, within Shewana3_2349 at 2827.782 kb on + strand, within Shewana3_2349 at 2827.806 kb on + strand at 2828.079 kb on - strand at 2828.314 kb on + strand, within Shewana3_2351 at 2828.432 kb on - strand, within Shewana3_2351 at 2828.507 kb on - strand at 2828.516 kb on + strand at 2828.631 kb on + strand at 2828.631 kb on + strand at 2828.639 kb on - strand at 2828.653 kb on + strand at 2828.661 kb on - strand at 2828.661 kb on - strand at 2828.698 kb on - strand at 2828.812 kb on + strand at 2828.822 kb on + strand at 2828.841 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 2,826,317 + Shewana3_2347 0.77 +4.0 2,826,594 - +0.3 2,826,930 - Shewana3_2348 0.45 +1.7 2,827,176 - Shewana3_2348 0.80 +0.2 2,827,277 + -1.6 2,827,430 + Shewana3_2349 0.23 +2.4 2,827,458 + Shewana3_2349 0.28 +2.4 2,827,500 + Shewana3_2349 0.36 +2.4 2,827,656 - Shewana3_2349 0.65 +1.2 2,827,782 + Shewana3_2349 0.89 +4.0 2,827,782 + Shewana3_2349 0.89 +3.0 2,827,782 + Shewana3_2349 0.89 -0.3 2,827,806 + +0.5 2,828,079 - -1.1 2,828,314 + Shewana3_2351 0.49 +0.2 2,828,432 - Shewana3_2351 0.87 +1.4 2,828,507 - +0.4 2,828,516 + +2.0 2,828,631 + +2.4 2,828,631 + +4.0 2,828,639 - +2.0 2,828,653 + +1.4 2,828,661 - -0.0 2,828,661 - +3.0 2,828,698 - +1.0 2,828,812 + +2.4 2,828,822 + -0.1 2,828,841 - +3.0
Or see this region's nucleotide sequence