Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2171 and Shewana3_2172 are separated by 65 nucleotides Shewana3_2172 and Shewana3_2173 overlap by 8 nucleotides Shewana3_2173 and Shewana3_2174 are separated by 66 nucleotides Shewana3_2174 and Shewana3_2175 are separated by 113 nucleotides
Shewana3_2171: Shewana3_2171 - electron transport complex, RnfABCDGE type, D subunit (RefSeq), at 2,604,281 to 2,605,330
_2171
Shewana3_2172: Shewana3_2172 - electron transport complex protein RnfG (RefSeq), at 2,605,396 to 2,606,022
_2172
Shewana3_2173: Shewana3_2173 - electron transport complex RsxE subunit (RefSeq), at 2,606,015 to 2,606,713
_2173
Shewana3_2174: Shewana3_2174 - DNA-(apurinic or apyrimidinic site) lyase / endonuclease III (RefSeq), at 2,606,780 to 2,607,415
_2174
Shewana3_2175: Shewana3_2175 - phage integrase family protein (RefSeq), at 2,607,529 to 2,608,851
_2175
Position (kb)
2606
2607 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2606.641 kb on + strand, within Shewana3_2173 at 2606.950 kb on + strand, within Shewana3_2174 at 2606.952 kb on + strand, within Shewana3_2174 at 2607.097 kb on + strand, within Shewana3_2174 at 2607.486 kb on - strand at 2607.621 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 2,606,641 + Shewana3_2173 0.90 +0.9 2,606,950 + Shewana3_2174 0.27 +2.9 2,606,952 + Shewana3_2174 0.27 +2.9 2,607,097 + Shewana3_2174 0.50 -0.3 2,607,486 - +1.6 2,607,621 + +2.3
Or see this region's nucleotide sequence