Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2160 and Shewana3_2161 are separated by 188 nucleotides Shewana3_2161 and Shewana3_2162 are separated by 249 nucleotides Shewana3_2162 and Shewana3_R0092 are separated by 125 nucleotides Shewana3_R0092 and Shewana3_R0093 are separated by 39 nucleotides
Shewana3_2160: Shewana3_2160 - diguanylate cyclase/phosphodiesterase with PAS/PAC and GAF sensor(s) (RefSeq), at 2,587,987 to 2,590,170
_2160
Shewana3_2161: Shewana3_2161 - hypothetical protein (RefSeq), at 2,590,359 to 2,590,646
_2161
Shewana3_2162: Shewana3_2162 - hypothetical protein (RefSeq), at 2,590,896 to 2,591,384
_2162
Shewana3_R0092: Shewana3_R0092 - tRNA-Tyr (RefSeq), at 2,591,510 to 2,591,594
_R0092
Shewana3_R0093: Shewana3_R0093 - tRNA-Tyr (RefSeq), at 2,591,634 to 2,591,718
_R0093
Position (kb)
2590
2591 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2589.374 kb on - strand, within Shewana3_2160 at 2589.419 kb on + strand, within Shewana3_2160 at 2589.612 kb on - strand, within Shewana3_2160 at 2589.841 kb on - strand at 2589.868 kb on - strand, within Shewana3_2160 at 2589.946 kb on - strand, within Shewana3_2160 at 2590.174 kb on + strand at 2590.350 kb on + strand at 2590.358 kb on - strand at 2590.515 kb on - strand, within Shewana3_2161 at 2590.675 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 2,589,374 - Shewana3_2160 0.64 +1.6 2,589,419 + Shewana3_2160 0.66 +3.9 2,589,612 - Shewana3_2160 0.74 -0.6 2,589,841 - +3.9 2,589,868 - Shewana3_2160 0.86 +3.9 2,589,946 - Shewana3_2160 0.90 +2.3 2,590,174 + +2.9 2,590,350 + -0.5 2,590,358 - -0.4 2,590,515 - Shewana3_2161 0.54 -0.8 2,590,675 - +2.9
Or see this region's nucleotide sequence