Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2145 and Shewana3_2146 are separated by 254 nucleotides Shewana3_2146 and Shewana3_2147 are separated by 25 nucleotides Shewana3_2147 and Shewana3_2148 are separated by 70 nucleotides Shewana3_2148 and Shewana3_2149 are separated by 20 nucleotides
Shewana3_2145: Shewana3_2145 - aminotransferase AlaT (RefSeq), at 2,569,796 to 2,571,010
_2145
Shewana3_2146: Shewana3_2146 - hypothetical protein (RefSeq), at 2,571,265 to 2,571,852
_2146
Shewana3_2147: Shewana3_2147 - deoxyguanosinetriphosphate triphosphohydrolase-like protein (RefSeq), at 2,571,878 to 2,573,218
_2147
Shewana3_2148: Shewana3_2148 - keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (RefSeq), at 2,573,289 to 2,573,930
_2148
Shewana3_2149: Shewana3_2149 - phosphogluconate dehydratase (RefSeq), at 2,573,951 to 2,575,777
_2149
Position (kb)
2571
2572
2573
2574 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2570.915 kb on - strand at 2571.042 kb on + strand at 2571.564 kb on + strand, within Shewana3_2146 at 2571.986 kb on + strand at 2572.052 kb on + strand, within Shewana3_2147 at 2572.423 kb on + strand, within Shewana3_2147 at 2572.664 kb on + strand, within Shewana3_2147 at 2572.779 kb on + strand, within Shewana3_2147 at 2572.996 kb on - strand, within Shewana3_2147 at 2573.153 kb on + strand at 2574.181 kb on - strand, within Shewana3_2149
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 2,570,915 - +2.3 2,571,042 + +1.3 2,571,564 + Shewana3_2146 0.51 +1.1 2,571,986 + +0.0 2,572,052 + Shewana3_2147 0.13 +3.9 2,572,423 + Shewana3_2147 0.41 +2.9 2,572,664 + Shewana3_2147 0.59 +1.6 2,572,779 + Shewana3_2147 0.67 +1.1 2,572,996 - Shewana3_2147 0.83 +2.3 2,573,153 + +0.4 2,574,181 - Shewana3_2149 0.13 +2.3
Or see this region's nucleotide sequence