Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1999 and Shewana3_2000 are separated by 83 nucleotides Shewana3_2000 and Shewana3_2001 are separated by 337 nucleotides Shewana3_2001 and Shewana3_2002 are separated by 34 nucleotides
Shewana3_1999: Shewana3_1999 - ATP-dependent DNA helicase RecQ (RefSeq), at 2,382,945 to 2,384,921
_1999
Shewana3_2000: Shewana3_2000 - ecotin (RefSeq), at 2,385,005 to 2,385,556
_2000
Shewana3_2001: Shewana3_2001 - hypothetical protein (RefSeq), at 2,385,894 to 2,386,331
_2001
Shewana3_2002: Shewana3_2002 - peptidyl-dipeptidase A (RefSeq), at 2,386,366 to 2,387,763
_2002
Position (kb)
2385
2386 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2384.705 kb on + strand, within Shewana3_1999 at 2384.705 kb on + strand, within Shewana3_1999 at 2384.705 kb on + strand, within Shewana3_1999 at 2384.713 kb on - strand, within Shewana3_1999 at 2384.862 kb on + strand at 2385.045 kb on + strand at 2385.154 kb on + strand, within Shewana3_2000 at 2385.162 kb on - strand, within Shewana3_2000 at 2385.172 kb on - strand, within Shewana3_2000 at 2385.174 kb on + strand, within Shewana3_2000 at 2385.273 kb on - strand, within Shewana3_2000 at 2385.514 kb on - strand at 2385.583 kb on + strand at 2385.591 kb on - strand at 2385.625 kb on - strand at 2386.019 kb on - strand, within Shewana3_2001 at 2386.136 kb on + strand, within Shewana3_2001 at 2386.145 kb on + strand, within Shewana3_2001 at 2386.238 kb on + strand, within Shewana3_2001 at 2386.384 kb on - strand at 2386.511 kb on - strand, within Shewana3_2002
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 2,384,705 + Shewana3_1999 0.89 -2.1 2,384,705 + Shewana3_1999 0.89 +2.5 2,384,705 + Shewana3_1999 0.89 +4.0 2,384,713 - Shewana3_1999 0.89 +0.7 2,384,862 + +1.0 2,385,045 + +2.5 2,385,154 + Shewana3_2000 0.27 +2.5 2,385,162 - Shewana3_2000 0.28 -1.9 2,385,172 - Shewana3_2000 0.30 -2.4 2,385,174 + Shewana3_2000 0.31 +1.1 2,385,273 - Shewana3_2000 0.49 +0.1 2,385,514 - -2.5 2,385,583 + +0.3 2,385,591 - +0.7 2,385,625 - +2.0 2,386,019 - Shewana3_2001 0.29 +2.0 2,386,136 + Shewana3_2001 0.55 +3.0 2,386,145 + Shewana3_2001 0.57 +2.4 2,386,238 + Shewana3_2001 0.79 +1.0 2,386,384 - +4.0 2,386,511 - Shewana3_2002 0.10 +1.7
Or see this region's nucleotide sequence