Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1767 and Shewana3_1768 are separated by 6 nucleotides Shewana3_1768 and Shewana3_1769 are separated by 352 nucleotides Shewana3_1769 and yebU are separated by 297 nucleotides
Shewana3_1767: Shewana3_1767 - DNA polymerase III, delta prime subunit (RefSeq), at 2,080,896 to 2,081,852
_1767
Shewana3_1768: Shewana3_1768 - type IV pilus biogenesis protein pilZ (RefSeq), at 2,081,859 to 2,082,185
_1768
Shewana3_1769: Shewana3_1769 - TatD family hydrolase (RefSeq), at 2,082,538 to 2,083,338
_1769
Shewana3_1770: yebU - rRNA (cytosine-C(5)-)-methyltransferase RsmF (RefSeq), at 2,083,636 to 2,085,168
yebU
Position (kb)
2082
2083
2084 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2081.887 kb on + strand at 2082.021 kb on - strand, within Shewana3_1768 at 2082.056 kb on + strand, within Shewana3_1768 at 2082.165 kb on + strand at 2082.180 kb on + strand at 2082.180 kb on + strand at 2082.188 kb on - strand at 2082.382 kb on + strand at 2082.464 kb on - strand at 2082.608 kb on + strand at 2082.608 kb on + strand at 2082.767 kb on + strand, within Shewana3_1769 at 2083.180 kb on + strand, within Shewana3_1769 at 2083.276 kb on + strand at 2083.926 kb on + strand, within yebU
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 2,081,887 + +0.3 2,082,021 - Shewana3_1768 0.50 +3.1 2,082,056 + Shewana3_1768 0.60 +3.1 2,082,165 + +2.1 2,082,180 + -0.2 2,082,180 + +0.3 2,082,188 - +0.1 2,082,382 + +0.8 2,082,464 - +0.4 2,082,608 + +2.6 2,082,608 + +1.4 2,082,767 + Shewana3_1769 0.29 +0.2 2,083,180 + Shewana3_1769 0.80 -1.0 2,083,276 + -0.5 2,083,926 + yebU Shewana3_1770 0.19 +3.1
Or see this region's nucleotide sequence