Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sucC and Shewana3_1713 overlap by 1 nucleotides Shewana3_1713 and Shewana3_1714 are separated by 379 nucleotides Shewana3_1714 and Shewana3_1715 are separated by 31 nucleotides
Shewana3_1712: sucC - succinyl-CoA synthetase subunit beta (RefSeq), at 2,016,832 to 2,017,998
sucC
Shewana3_1713: Shewana3_1713 - succinyl-CoA synthetase (ADP-forming) alpha subunit (RefSeq), at 2,017,998 to 2,018,870
_1713
Shewana3_1714: Shewana3_1714 - GreA/GreB family elongation factor (RefSeq), at 2,019,250 to 2,019,657
_1714
Shewana3_1715: Shewana3_1715 - GCN5-related N-acetyltransferase (RefSeq), at 2,019,689 to 2,020,201
_1715
Position (kb)
2017
2018
2019 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2017.453 kb on + strand, within sucC at 2017.864 kb on + strand, within sucC at 2018.052 kb on + strand at 2018.133 kb on + strand, within Shewana3_1713 at 2018.154 kb on + strand, within Shewana3_1713 at 2018.964 kb on + strand at 2018.972 kb on - strand at 2019.001 kb on + strand at 2019.070 kb on + strand at 2019.077 kb on + strand at 2019.078 kb on - strand at 2019.080 kb on - strand at 2019.151 kb on - strand at 2019.199 kb on - strand at 2019.204 kb on - strand at 2019.259 kb on - strand at 2019.496 kb on + strand, within Shewana3_1714 at 2019.523 kb on + strand, within Shewana3_1714 at 2019.665 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 2,017,453 + sucC Shewana3_1712 0.53 +1.3 2,017,864 + sucC Shewana3_1712 0.88 +2.6 2,018,052 + +3.1 2,018,133 + Shewana3_1713 0.15 +0.9 2,018,154 + Shewana3_1713 0.18 +3.9 2,018,964 + +2.8 2,018,972 - -1.2 2,019,001 + +1.1 2,019,070 + +0.8 2,019,077 + +2.5 2,019,078 - +1.5 2,019,080 - +0.5 2,019,151 - +3.1 2,019,199 - +3.1 2,019,204 - +3.1 2,019,259 - -0.4 2,019,496 + Shewana3_1714 0.60 +0.1 2,019,523 + Shewana3_1714 0.67 -0.8 2,019,665 + -0.0
Or see this region's nucleotide sequence